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Many-faceted Studies on Roles of Extracellular Matrix in Development of Glaucoma

Research Project

Project/Area Number 16591777
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

TAWARA Akihiko  University of Occupational and Environmental Health, Japan, School of Medicine, Professor (90117169)

Co-Investigator(Kenkyū-buntansha) KUBOTA Toshiaki  University of Occupational and Environmental Health, Japan, School of Medicine, Professor (30205140)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsglaucoma / trabecular meshwork / congenital microcoria / extracellular matrix / Bevacizumab / TGF-β / angiopoietin-II / NMDA receprer / 免疫組織化学 / プロテオグリカン / matrix metalloproteinase / 会報隅角緑内障 / トリアムシノロンアセトニド / アンギオポイエチン-2 / 発達緑内障 / ステロイド緑内障 / 血管新生緑内障 / Axenfeld-Rieger症候群 / FOXC1遺伝子
Research Abstract

We immunohistochemically stained the extracellular matrixes in the trabecular meshwork obtained by trabeculectomy from 3 eyes of2 patients with steroid-induced glaucoma. The results indicated that there were increased accumulations of extracellular matrix including basement membrane-like materials in the outer part of the trabecular meshwork. The results also showed that type IV collagen, heparan sulphate-type proteoglycan and fibronectin increase in the trabecular meshwork. From this study, it was suggested that increase of the extracellular matrix in the trabecular meshwork may one of the causes of intraocular elevation in steroid-induced glaucoma.
1. We examined immunohistochemically expression of TGF-βin the trabecular meshwork from eyes with primary open angle glaucoma ( POAG) and exfoliation glaucoma ( PEX) as well as the concentration of the cytokine in the aqueous humor by the ELISA methods. There were heavy accumulations of TGF-β in the trabecular meshwork from both POAG and PE … More X compared with from normal control. The concentration of the cytokine in the aqueous humor from POAG was statistically higher than that from cataract patients.
2. We examined morphological changes in the trabecular meshwork from neovascular glaucoma with intravitreous injection of Bevacizumab, which is an anti-VEGF antibody. The results indicated that Bevacizumab could reduce the number of fenestration in the newly formed vessels of neovascular glaucoma.
3. We examined immunohistochemically expression of neuropilin-1 and angiopoietin-II in the trabecular meshwork from eyes with neovascular glaucoma. The results indicated that there were depositions of angiopoietin-II in the trabecular meshwork from eyes with neovascular glaucoma
4. We studied the effect of anti-glaucoma drug of nipradilol, both α- and β-blocker, on the production of MMPs and TIMPs in cultured human trabecular cells by using the Western blot technique. From this study, it was suggested that nipradilol has the cultured human trabecular meshwork cells increase the production of TIMP-2.
5. We examined whether central corneal thickness ( CCT) and corneal curvature radius( CCR) have influences on the concordance of intraocular pressure ( IOP) readings taken with non-contact tonometer ( NCT) and Goldmann applanation tonometer ( GAT) by measuring CCR, CCT, and IOP using NCT and GAT. The results indicated that CCT and CCR could influence the discordance of IOP readings taken with NCT and GAT.
6. We examined the direct effects of anti-glaucoma drugs on NMDA receptor function using a Xenopus oocytes system and electrophysiological techniques. The results suggested that timolol and betaxolol may have an additional role that they directly inhibit NMDA receptor function possibly via N616 of the NR1a subunit.
7. We followed up three subjects descended from a family with autosomal dominant congenital microcoria and goniodysgenesis for more than 25 years. Two of these subjects developed late onset developmental glaucoma in both eyes during the 25-years follow-up period. It is, therefore, suggested that congenital microcoria are frequently associated with the incidence of late onset developmental glaucoma. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (42 results)

All 2007 2006 2005 2004 Other

All Journal Article (30 results) Book (12 results)

  • [Journal Article] 冷凍保存羊膜移植を併用した線維柱帯切除術2007

    • Author(s)
      新田 憲和, 田原 昭彦
    • Journal Title

      臨床眼科 61・3

      Pages: 451-455

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 急性原発閉塞隅角症の眼圧コントロールに対する超音波水晶体乳化吸引, 眼内レンズ挿入術の成績2007

    • Author(s)
      山下 美恵, 田原 昭彦
    • Journal Title

      あたらしい眼科 24・5

      Pages: 673-676

    • NAID

      10022614844

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 産業医科大学における血管新生緑内障の治療成績2007

    • Author(s)
      森田 啓文, 田原 昭彦
    • Journal Title

      あたらしい眼科 24・7

      Pages: 947-951

    • NAID

      10022615350

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effectiveness of Phacoemulsification with Intraocular Lens Implantaion in Controling Intraocular Pressure in Acute Primary Angle-Closure2007

    • Author(s)
      Nitta N., Hirose N., Tawara A., Kubota T.
    • Journal Title

      Japanese journal of clinical ophthalmology 61・3

      Pages: 451-455

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effectiveness of Phacoemulsification with Intraocular lens Implantaion in Controling Intraocular Pressure in Acute Primary Angle-Closure2007

    • Author(s)
      Yamashita M., Kubota T., Morita H., Harada Y., Tawara A., Toguri I., Miyamura N.
    • Journal Title

      Journal of the eye 24・5

      Pages: 673-676

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Therapeutic results in neovascular glaucoma2007

    • Author(s)
      Morita H., Hirose N., Nasu T., Kubota T., Tawara A.
    • Journal Title

      Journal of the eye 24・7

      Pages: 947-951

    • NAID

      10022615350

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 白内障, 緑内障および網膜硝子体手術に対するクリニカルパス導入の効果2006

    • Author(s)
      久保田 敏昭, 田原 昭彦
    • Journal Title

      日本眼科学会雑誌 110・1

      Pages: 25-30

    • NAID

      10017326819

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 眼痛を伴う水庖性角膜症に対し羊膜移植術が有効であった2例2006

    • Author(s)
      小池 直栄, 田原 昭彦
    • Journal Title

      日本眼科紀要 57・3

      Pages: 209-212

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ultrastructure of the trabecular meshwork in secondary glaucomaeyes after intravitreal triamcinolone acetonide2006

    • Author(s)
      Kubota, T., Tawara, A.
    • Journal Title

      Journal of Glaucoma 15・2

      Pages: 117-119

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of Latanoprost on the diurnal variations in the intraocular and ocular perfusion pressure in normal tension glaucoma2006

    • Author(s)
      Ishibashi, S., Tawara, A.
    • Journal Title

      Journal of Glaucoma 15・5

      Pages: 354-357

    • NAID

      10014261944

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 緑内障眼における視神経乳頭形態の長期経時的変化2006

    • Author(s)
      廣石 悟朗, 久保田 敏昭
    • Journal Title

      臨床眼科 60・3

      Pages: 329-333

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 数年間著明な眼圧季節変動を示した太田母斑に併発した発達緑内障の1例2006

    • Author(s)
      佐々木 徹, 久保田 敏昭
    • Journal Title

      あたらしい眼科 23・6

      Pages: 817-820

    • NAID

      10018835812

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of implementing clinical Pathways for the Care of Patients Undergoing Ophthalmic Surgery for Cataract, and Vitreoretinal Disorder2006

    • Author(s)
      Kubota T., Nakamura A., Matsuo J., Mochizuki Y., Onizuka N., Suyama Y., Miyazaki M., Tawara A.
    • Journal Title

      Acta Societatis Ophthalmologicae Japonicae 110・1

      Pages: 25-30

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effectiveness of Amniotic Membrane Transplantation in Two Cases of Bullous Keratopathy Eye Pain2006

    • Author(s)
      Koike N., Hirose N., Koike I., Kubota T., Tawara A.
    • Journal Title

      Folia ophthalmologica Japonica 57・3

      Pages: 209-212

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ultrastructure of the trabecular meshwork in secondary glaucoma eyes after intravitreal triamcinolone acetonide.2006

    • Author(s)
      Kubota T., Okabe H., Hisatomi T., Yamakiri K., Sakamoto T., Tawara A.
    • Journal Title

      Journal of Glaucoma 15・2

      Pages: 117-119

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of Latanoprost on the Diurnal Variations in the Intraocular and Ocular Perfusion Pressure in Normal Tension Glaucoma.2006

    • Author(s)
      Ishibashi S., Hirose N., Tawara A., Kubota T.
    • Journal Title

      Journal of Glaucoma 15・5

      Pages: 354-357

    • NAID

      10014261944

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of Latanoprost on the diurnal variations in the intraocular and ocular perfusion pressure in normal tension glaucoma2006

    • Author(s)
      Ishibashi, S., Tawara, A.
    • Journal Title

      J Glaucoma 15・5

      Pages: 354-357

    • NAID

      10014261944

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Ultrastructure of the trabecular meshwork in secondary glaucomaeves after intravitreal triamcinolone acetonide2006

    • Author(s)
      Kubota, T., Tawara, A.
    • Journal Title

      J Glaucoma 15・2

      Pages: 117-119

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Congenital microcoria associated with late-onset developmentalglaucoma2005

    • Author(s)
      Tawara, A.
    • Journal Title

      Journal of Glaucoma 14・5

      Pages: 409-413

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The prevalence of pseudoexfoliation syndrome in a Japanese population : the Hisayama Study2005

    • Author(s)
      Miyazaki, M., Kubota, T.
    • Journal Title

      Journal of Glaucoma 14・6

      Pages: 482-484

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Congenital microcoria associated with late-onset developmental glaucoma.2005

    • Author(s)
      Tawara A., Itou K., KubotaT., Harada Y., Tou N., Hirose N.
    • Journal Title

      Journal of Glaucoma 14・5

      Pages: 409-413

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The Prevalence of Pseudoexfoliation Syndrome in a Japanese Population The Hisayama Study.2005

    • Author(s)
      Miyazaki M., Kubota T., Kubo M., Kiyohara Y., Iida M., Nose Y., Ishibashi T.
    • Journal Title

      Journal of Glaucoma 14・6

      Pages: 482-482

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Congenital microcoria associated with late-onset developmental glaucoma2005

    • Author(s)
      Tawara, A.
    • Journal Title

      Journal of Glaucoma 14・5

      Pages: 409-413

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 房水の産生と流出2005

    • Author(s)
      田原 昭彦
    • Journal Title

      あたらしい眼科 22・別巻

      Pages: 23-26

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 特集【加齢変化による眼病態】3.毛様態・隅角の変化2005

    • Author(s)
      田原 昭彦
    • Journal Title

      眼科 47・8

      Pages: 1055-1062

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 眼科臨床に必要な解剖生理 IV.虹彩・房水 4.隅角の解剖2005

    • Author(s)
      田原 昭彦
    • Journal Title

      眼科プラクティス 6

      Pages: 138-143

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The prevalence of pseudoexfoliation syndrome in a Japanese population : the Hisayama Study2005

    • Author(s)
      Miyazaki, M.
    • Journal Title

      Journal of Glaucoma 14・6

      Pages: 482-484

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 正常眼圧緑内障患者の眼圧日内変動に対するラタノプラストの効果2004

    • Author(s)
      石橋 真吾, 田原 昭彦
    • Journal Title

      あたらしい眼科 21・12

      Pages: 1693-1696

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of Latanoprost on Diurnal Variations in Intraocaular and Ocular Perfusion Pressure in Normal-Tension Glaucoma2004

    • Author(s)
      Ishibashi S., Hirose N., Tawara, A
    • Journal Title

      Journal of the Eye 21・12

      Pages: 1693-1696

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Ultrastructure of the trabecular meshwork in secondary glaucoma eyes after intravitreal triamcinolone acetonide

    • Author(s)
      Kubota, T.
    • Journal Title

      Journal of Glaucoma (in press)

    • Related Report
      2005 Annual Research Report
  • [Book] 眼科プラクティス 第8巻 【いますぐ役立つ眼病理】2006

    • Author(s)
      田原 昭彦
    • Total Pages
      1
    • Publisher
      文光堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 眼科プラクティス 第8巻 【いますぐ役立つ眼病理】2006

    • Author(s)
      久保田 敏昭
    • Total Pages
      4
    • Publisher
      文光堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 眼科インストラクションコース7 緑内障 細隙灯顕微鏡診断完全マスター2006

    • Author(s)
      久保田 敏昭
    • Total Pages
      4
    • Publisher
      メディカルビュー
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 眼科インストラクションコース7 緑内障 細隙灯顕微鏡診断完全マスター2006

    • Author(s)
      田原 昭彦
    • Total Pages
      4
    • Publisher
      メディカルビュー
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 眼科プラクティス 第11巻 【緑内障診療の進めかた】2006

    • Author(s)
      田原 昭彦
    • Total Pages
      4
    • Publisher
      文光堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 眼科プラクティス11緑内障診療の進めかた2006

    • Author(s)
      田原 昭彦
    • Publisher
      I病型別診断と治療 17.早発型発達緑内障。1)隅角の発生と病理
    • Related Report
      2006 Annual Research Report
  • [Book] 眼科プラクティス 8 いますぐ役立つ眼病理2006

    • Author(s)
      田原 昭彦、久保田 敏昭
    • Publisher
      V隅角・線維柱帯と水晶体 組織を見る際の線維柱帯切除術の要点、検体の固定と切り出し
    • Related Report
      2006 Annual Research Report
  • [Book] あたらしい眼科2005

    • Author(s)
      田原 昭彦
    • Total Pages
      4
    • Publisher
      メディカル葵出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 眼科2005

    • Author(s)
      田原 昭彦
    • Total Pages
      8
    • Publisher
      金原出版株式会社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 眼科プラクティス 第6巻 眼科臨床に必要な解剖生理2005

    • Author(s)
      田原 昭彦
    • Total Pages
      6
    • Publisher
      文光堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] Ophthalmic Foresight, 92004

    • Author(s)
      田原 昭彦
    • Total Pages
      2
    • Publisher
      株式会社 フアーマインターナショナル
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 眼科インストラクションコース 7 緑内障 細隙灯顕微鏡診断完全マスター

    • Author(s)
      田原 昭彦
    • Publisher
      角結膜の診かた 発達緑内障に関連する角膜の異常所見
    • Related Report
      2006 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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