| Project/Area Number |
16591778
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | National Tokyo Medical Center (National Institute of Sensory Organs) |
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Principal Investigator |
YAMADA Masakazu National Tokyo Medical Center (National Institute of Sensory Organs), Director, 視覚研究部, 部長 (50210480)
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| Co-Investigator(Kenkyū-buntansha) |
MOCHIZUKI Hiroshi National Tokyo Medical Center (National Institute of Sensory Organs), Research Resident, 視覚研究部・研究員 (60286565)
ARITA Reiko National Tokyo Medical Center (National Institute of Sensory Organs), Research Resident, 視覚研究部・研究員 (00338120)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | corneal endothelium / bullous keratopathy / pharmacological treatment / steroids / insulin / cornea / endothelium / Na-K ATPase / protein kinase C / steroid / substance P / IGF-1 |
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| Research Abstract |
Recent studies have suggested that corticosteroids and insulin affect the pump function of corneal endothelium. Pump function of corneal endothelial cells is mainly dependent on their Na, K-ATPase activity. This study was undertaken to test whether dexamethasone and insulin influence the pump function and Na, K-ATPase activity of mouse corneal endothelial cells.
C3H cell line derived from mouse corneal endothelial cells was used in the study. Various concentrations of dexamethasone and insulin were added to confluent C3H cells for 48 hours. The Na, K-ATPase activity was estimated by phosphorus determination with ammonium molybdate, which was resulting from ATP hydrolysis. Dexamethasone caused a dose-dependent activation of Na, K-ATPase at concentration from 10^<-8>M to 10^<-5>M. The Na, K-ATPase activity reached 368% of control when 10^<-5>M of dexamethasone was added. Insulin also caused an activation of Na, K-ATPase at concentration of 10^<-7>M. Our results indicated that dexamethasone and insulin increased the pump function of cultured corneal endothelial cells by activating Na, K-ATPase. Corticosteroids and insulin may be effective in treating corneal edema by activating the function of corneal endothelium.
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