| Project/Area Number |
16591790
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
KITAGAWA Hiroaki St.Marianna University School of Medicine, Pediatric Surgery, Associate Prof., 医学部, 助教授 (80153097)
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| Co-Investigator(Kenkyū-buntansha) |
KOIKE Junki St.Marianna Univ., Pathology, Assistant Prof., 医学部, 講師 (40308440)
WAKISAKA Munechika St.Marianna Univ., Pediatric Surg., Lecturer, 医学部, 講師 (30267596)
SATO Hideaki St.Marianna Univ., Pediatric Surg., Lecturer, 医学部, 助手 (70339852)
NAKADA Koonosuke St.Marianna Univ., Pediatric Surgery, Professor, 医学部, 教授 (70081734)
黒田 志穂 聖マリアンナ医科大学, 医学部, 助手 (30386966)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Fetal Surgery / Multicystic Dysplastic Kidney (MCDK) / Fetal Hydronephrosis / Obstructive Uropathy / 多襄胞性異型性腎(MCDK) |
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| Research Abstract |
Purpose : The long-term outcome for children after antenatal intervention for obstructive uropathy is disappointing. We reported that renal dysplastic changes are well-established 3 weeks after obstruction in a fetal lamb model. We used this model to explore renal development and bladder function after fetal intervention.
Methods : We created an obstructive uropathy in fetal lambs at 60 days gestation by legating the urethra and urachus. A vesicostomy (female) or urethrostomy (male) were performed 21 days later. The fetuses were sacrificed at term (145 days) and bladder volume and were measured. The urinary tract was processed for histological examination.
Results : Forty fetuses created an obstructive uropathy. Eleven miscarried or were stillborn. Twenty-nine survived and twenty-four had a successful shunt with a small bladder (8±5ml) compared to controls (71±19ml), p<0.05. Shunted bladders had poor compliance. Histologically, they had thickened sub mucosal connective tissue with hypertrophied muscle. Histology of the renal tissue demonstrated relatively well-preserved renal architecture with reduced nephrons mass (oligonephronia) in nine lambs and multicystic dysplastic change (MCDK) in three. Thirteen (54%) had normal nephrogenesis or slightly dilated pelvis as a Hydronephrosis.
Conclusions : In our model, shunt operations after obstructive uropathy fail to preserve bladder function. Shunting ameliorated the development of cystic dysphasia but half of the lambs had oligonephronia or MCDK. These might develop renal failure later in life but 54% had normal nephrogenesis after shunting.
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