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Research for wound healing using an artificial skin model composed with cultured human skin cells.

Research Project

Project/Area Number 16591799
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionSHOWA UNIVERSITY

Principal Investigator

KAO Bunsho  SHOWA UNIV., PLASTIC SUGRGERY, LECTURER, 医学部, 講師 (10327893)

Co-Investigator(Kenkyū-buntansha) HOSAKA Yoshiaki  SHOWA UNIV., PLASTIC SUGRGERY, PROFESSOR, 医学部, 教授 (40156998)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsARTIFICIAL SKIN / WOUND HEALING / HUMAN SKIN MODEL / PLACENTAL EXTRACT
Research Abstract

Artificial skin cultures made using cultured human skin cells have attracted attention as experimental models, as well as for clinical applications. However artificial skin has few chemical articles that undergo damage and observed progress. We created a number of artificial skin models. These artificial models were irradiated with a CO2 laser at various strengths. We evaluated the degree of damage and the time course of tissue regeneration in these models using pathological and immunohistochemical techniques.
With mild irradiation conditions, epidermal keratinocytes in the deep layer survived. The damaged surface was then repaired by the growth of undamaged epidermal keratinocytes in a short time. In contrast, when the dermal layer was damaged, a longer time period was required for keratinocyte regeneration. From these results, we conclude that surviving epidermal keratinocytes in irradiated skin sites are important for skin tissue regeneration. When laser irradiation is mild and keratinocytes remain at the irradiated area, epidermal keratinocytes develop many layers and the repaired epidermis is thick and firm. In contrast, when the dermal cells are damaged, the repaired epidermis is weaker with fewer layers of epidermal keratinocytes.
We also studied the effects of human placental extract by using an artificial skin model with laser irradiation. The samples were irradiated with a CO2 laser and cultured in a medium with 1% HPE, 0.1% HPE, 0% HPE (control). The conclusion was that HPE produced a specific density affect as an accelerator of wound healing, but successive amounts of HPE produced a specific density affect as an inhibitor.
Our observations resemble physiological wound healing processes within the living body. We believe that this artificial skin model is unique and useful for experimental studies of skin regeneration after irradiation with various lasers.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (3 results)

All 2005 2004

All Journal Article (3 results)

  • [Journal Article] 組織工学を応用した皮膚代替物2005

    • Author(s)
      加王 文祥
    • Journal Title

      昭和医学会雑誌 65巻第4号

      Pages: 299-301

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Skin Iquivalent with Tissue Engineering Technic2005

    • Author(s)
      BUNSHO KAO
    • Journal Title

      J. of the showa medical association 65(4)

      Pages: 299-299

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Imaging wound healing using optical coherence tomography and multi photon microscopy in an in vitro skin-equivalent tissue model2004

    • Author(s)
      Alvin T.Yeh, Bunsho Kao, Woong Gvn lung et al.
    • Journal Title

      Journal of Biomedical Optics 9(2)

      Pages: 248-253

    • Related Report
      2004 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

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