| Project/Area Number |
16591804
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
IWAKIRI Itaru Nippon Medical School, Faculty of Medicine, Research Associate, 医学部, 助手 (50307899)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUNO Hiroshi Nippon Medical School, Faculty of Medicine, Associate Professor, 医学部, 助教授 (80343606)
AKAISHI Satoshi Nippon Medical School, Faculty of Medicine, Research Associate, 医学部, 助手 (10373010)
MURAKAMI Masahiro Nippon Medical School, Faculty of Medicine, Associate Professor, 医学部, 助教授 (00239500)
HYAKUSOKU Hiko Nippon Medical School, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (00165135)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | mesenchymal stem cells / adipose tissue / skin / regenerative medicine / tissue engineering |
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| Research Abstract |
Adipose derived stem cells (ASCs) are known to be multi-potent in that they differentiate into diverse cell types including fibroblasts, osteoblasts, chondrocytes, and adipocytes. The aim of this study was to evaluate potential accelerating effects of ASCs with collagen matrix sponge for repairing chronic skin ulcer.
Before in vivo experiment, proliferation potential of ASCs in collagen sponge was tested in vitro. The results showed that ASCs maintain the proliferation potential in collagen sponge until 7 days after seeding.
Next, full-thickness skin defects were made on the back of F344 rats and mitomycin C solution (1 mg/ml in saline) was applied onto wounds for 10 min to create intractable ulcers. After thoroughly washing out the mitomycin C, ASCs with collagen sponge were applied. Specimens were harvested after 7 and 14 days of implantation for histological analysis. The thickness of the granulation tissue and the density of capillary were both statistically higher in experiment group than in the control group at each time point.
Finally, similar experiment was carried out using db/db mice to test if the similar phenomenon would happen in diabetic model.
The results showed that the healing process was also better in the experiment group.
These results indicate that transplantation of ASCs with collagen sponge is effective for repairing intractable skin uclers.
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