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Study of the life span and cell death of osteoclasts with cell fusion of green mouse

Research Project

Project/Area Number 16591819
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionHokkaido University

Principal Investigator

DOMON Takanori  Hokkaido University, Graduate School of Dental Medicine, associate professor, 大学院・歯学研究科, 助教授 (50217618)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Tsuneyuki  Hokkaido University, Gradate School of Dental Medicine, associate professor, 大学院・歯学研究科, 助教授 (80200822)
TAKAHASHI Shigeru  Hokkaido University, Graduate School of Dental Medicine, instructor, 大学院・歯学研究科, 助手 (70241338)
MITOME Masato  Hokkaido University, Hokkaido University Hospital, lecturer, 講師 (50261318)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsosteoclasts / odontoclasts / multinucleation / cell fusion / cell death / apoptosis / necrosis / 延命 / 細胞寿命
Research Abstract

Life span and cell death of multinucleated osteoclasts and odontoclasts remain unclear. The present study examines survival of osteoclasts after cell fusion and aims to clarify the cell death of these multinucleated cells.
According to the schedule of the research on 2004, the following results are shown : in culture, osteoclasts are formed from mouse bone marrow cells in the presence of M-CSF and RANKL. When stopping the addition of M-CSF and RANKL to cell culture, there are few cell fusion between osteoclasts and cells gradually degenerate. When adding bone marrow cells of green mouse, M-CSF, and RANKL to culture dish, cell fusion between osteoclasts and mononuclear cells with green nuclei occurs. After fixation, a mix of normal nuclei and green nuclei in an osteoclast was observed by fluorescent microscopy, and most cell without green nuclei were dying.
According to the schedule of the research on 2005, the following results are shown : to know cell death of odontoclasts in normal condition, human deciduous teeth were used in this study. For immunohistochemistry (IHC), TUNEL and TRAP activities were detected on paraffin section. For transmission electron microscope (TEM), 0.5μm-thick serial semithin sections were made, and ultrastructures of apoptotic odontoclasts were examined. By IHC, nuclei of TRAP-positive odontoclasts did not show TUNEL reaction, however there were TRAP-positive structures with TUNEL-positive nuclei. By TEM, characteristic nuclear fragments were seen in odontoclasts, and there were typically electron dense condensed mitochondria in the cytoplasm. There were odontoclasts with swelling of cytoplasm and cytoplasmic compartments ; mitochondria and rough-surfaced endoplasmic reticulum. These IHC and TEM results show that cell death of odontoclasts under physiological root resorption of human deciduous teeth reveals two patterns of apoptosis and necrosis.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (6 results)

All 2005 2004

All Journal Article (6 results)

  • [Journal Article] Features of the clear zone of odontoclasts in the Chinook Salmon (Oncorhynchus tshawytscha)2005

    • Author(s)
      Domon T
    • Journal Title

      Anatomy and Embryology 210(In press)

    • NAID

      120002451631

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Features of the clear zone of odontoclasts in the Chinook salmon (Oncorhynchus tshawytscha)2005

    • Author(s)
      Domon T
    • Journal Title

      Anatomy and Embryology 210 (in press)

    • NAID

      120002451631

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Features of the clear zone of odontoclasts in the Chinook salomon2005

    • Author(s)
      Domon T
    • Journal Title

      Anatomy and Embryology 210(In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Odontoclasts in the Chinook salmon differ from mammalian odontoclasts by exhibiting a great proportion of cells with high nuclei number2004

    • Author(s)
      Domon T
    • Journal Title

      Anatomy and Embryology 209

      Pages: 119-128

    • NAID

      120002451630

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] odontoclasts in the Chinook salomon differ from mammalian odontoclasts by exhibiting a great proportion of cells with high nuclei number2004

    • Author(s)
      Domon T
    • Journal Title

      Anatomy and Embryology 209

      Pages: 119-128

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Odontoclasts in the Chinook salmon differ from mammalian odontoclasts by exhibiting a great proportion of cells with high nuclei number2004

    • Author(s)
      Domon T, et al.
    • Journal Title

      Anatomy and Embryology 209

      Pages: 119-128

    • NAID

      120002451630

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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