| Project/Area Number |
16591820
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Chiba University |
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Principal Investigator |
OGAWARA Katsunori CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, INSTRUCTOR, 大学院・医学研究院, 助手 (20372360)
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| Co-Investigator(Kenkyū-buntansha) |
ONO Kanae CHIBA UNIVERSITY, HOSPITAL, INSTRUCTOR, 医学部附属病院, 助手 (60344983)
BUKAWA Hiroki CHIBA UNIVERSITY, HOSPITAL, ASSISTANT PROFESSOR, 医学部附属病院, 講師 (80173558)
UZAWA Katsuhiro CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 大学院・医学研究院, 助教授 (30302558)
TANZAWA Hideki CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, PROFESSOR, 大学院・医学研究院, 教授 (50236775)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | oral carcinoma / Ca^<2+> binding protein / microarray / gene expression profile / predictive carcinogenesis / Ca結合タンパク / マイクロアレイ解析 / ATP2A遺伝子群 / ATP2B遺伝子群 / S100遺伝子群 |
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| Research Abstract |
We examined many oral carcinoma, precancerous lesions, and normal tissues for the expression status of Ca^<2+> binding protein genes using our in-house cDNA microarray consisting 2201 cDNA clones Affymetrix GeneChip^<TM> consisting 48000 probes. The data of microarray analyses were confirmed by quantitative real time PCR.
Twenty seven genes showed differential over-expression and 83 genes showed differential down expression in oral carcinoma, compared with those in normal tissue. Out of Ca^<2+> pump genes of endoplasmic reticulum ATP2a family genes were detected their suppressed expression. Expression of ATP2b family genes, which work as Ca^<2+> pump on the cell membrane, and calsequestrin-1 gene, which maintains the concentration of Ca^<2+> in the endplasmic reticulum, were also detected to be suppressed. The suppression of gene expression of S100 family genes, which play many roles, were also revealed.
These results suggested that the carcinogenesis may resulting in slow switching of many systems in the carcinoma cells.
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