Establishment and characterization of immortalized human ameloblastoma cell lines, HAM1 and 2
| Project/Area Number |
16591850
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tokyo Medical and Dental University (2005)
Osaka Dental University (2004) |
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Principal Investigator |
KOZAKI Ken-ichi Tokyo Medical and Dental University., Medical Research Institute, Graduate School, Assistant Professor, 難治疾患研究所, 特任助教授 (50270715)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | ameloblastoma / odontogenic tumor / postoperative recurrence / model system / immortalization |
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| Research Abstract |
Ameloblastoma is a benign and the most common odontogenic neoplasm, whereas high recurrence rates and possible malignant development have been reported. In the present study, we established two immortalized cell lines, termed HAM1 and 2, by transfecting of human telomerase reverse transcriptase (hTERT) gene and human papillomavirus-16 (HPV16) E6/E7 genes into primary in vitro outgrowths from follicular and desmoplastic ameloblastoma specimens. Both lines could growth in vivo not only as a result of orthotopic (HAM1, 100% ; HAM2, 100%) but also of ectopic (HAM1, 33% ; HAM2, 100%) implantation. The histological findings of these inoculated tissues showed that these cell lines retained the clinical manifestations of ameloblastoma well. These cell lines are, to the best of our knowledge, the first in vitro / in vivo model system that can form recognizable tissue structures as human ameloblastoma in vivo. Then, we analyzed expression of some cancer-related molecules in these cell lines, and selected a candidate gene showed the highest expression frequency (100%) in RT-PCR analysis of eight primary ameloblastoma cases. An immunohistochemical study also demonstrated that the production of the candidate gene was considerably increased in 34 of 65 primary ameloblastoma specimens (52.3%). Moreover, a selective inhibitor for this candidate gene showed significant inhibitory effects on these cell lines in vitro and in vivo. Our study clearly supports that products of this candidate gene can make one of the molecular targets in treatment of ameloblastoma.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Identification of specific autoantigens in Sjogren's syndrome by SEREX.2005
Author(s)
Uchida, K., Akita, Y., Matsuo, K., Fujiwara, S., Nakagawa, A., Kazaoka, Y., Hachiya, H., Naganawa, Y., Oh-iwa, I., Ohura, K., Saga, S., Kawai, T., Matsumoto, Y., Shimozato, K., Kozaki, K.
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Journal Title
Immunology(Corresponding author) 116
Pages: 53-63
Description
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[Journal Article] A role of activated Sonic hedgehog signaling for the cellular proliferation of oral squamous cell carcinoma cell line.2004
Author(s)
Nishimaki, H., Kasai, K., Kozaki, K., Takeo, T., Ikeda, H., Saga, S., Nitta, M., Itoh, G.
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Journal Title
Biochem.Biophys.Res.Commun. 314
Pages: 313-320
Description
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[Journal Article] Cyclooxygenase-2 is a possible target of treatment approach in conjunction with photodynamic therapy for various disorders in skin and oral cavity.2004
Author(s)
Akita, Y., Kozaki, K., Nakagawa, A., Saito, T., Ito, S., Tamada, Y., Fujiwara, S., Nishikawa, N., Uchida, K., Yoshikawa, K., Noguchi, T., Miyaishi, O., Shimozato, K., Saga, S., Matsumoto, Y.
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Journal Title
Br.J.Dermatol.(Corresponding author) 151
Pages: 472-480
Description
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