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Three-dimensional structural studies of bone tissues by ion-etching-scanning electron microscopy.

Research Project

Project/Area Number 16591852
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionFukuoka Dental College

Principal Investigator

NAGATO Toshikazu  Fukuoka Dental College, Department of Morphological Biology, Professor, 歯学部, 教授 (80084284)

Co-Investigator(Kenkyū-buntansha) SATO Atsuko  Fukuoka Dental College, Department of Morphological Biology, Professor, 歯学部, 教授 (20099047)
SUGIMOTO Yukitaka  Fukuoka Dental College, Department of Morphological Biology, Assistant Professor, 歯学部, 講師 (40162896)
YAHIRO Junko  Fukuoka Dental College, Department of Morphological Biology, Assistant, 歯学部, 助手 (60105682)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsresin secton / ion-etching / scanning electron microscope / three-dimensional structure / blood vessel / bone tissue / periodontium / immuno-SEM / エポキシ樹脂 / 微小血管網
Research Abstract

Three studies were performed in this research project; (1) Three-dimensional structures of the bone tissues of fumerus and alveolar bone and periodontium of mandible of rats were studied with the resin section-ion etching (IE)-scanning electron microscopy (SEM) and energy filter (EF) transmission electron microscope (TEM)-TEM tomography. (2) Immuno histo and cyto-chemistry of rat salivary glands including parotid and von Ebner's gland was performed by using IE-SEM method. (3) Stem cell factors on cultured bone marrow cells which were harvested from mouse tibiae were also examined.
In IE-SEM studies, it was suggested that the structure of inner circumferential lamellae was clear, bat arrangement of some collagen fibrils were parallel, some oblique or some crossing as well as osteocytes. On the other hand, in alveolar bone, lamellar structure was absent, and osteocyte arrangement were irregular. In FETEM-TEM tomography study, lamellar structure were clearly observed in the semi-thin secti … More ons of the inner circumferential lamellae in fumerus, but not observed in the alveolar bone. Complicated arrangement of collagen fibrils were alse observed in the semi-thin sections of the periodontium.
The ability to apply both light and scanning electron microscopy to immunostained specimens was examined with rat salivary gland. Immunoscanning electron micrographs of the sections which were immunohistochemically stained with anti-amylase and immunogold in association with silver enhancement techniques showed that secretory granules in the secretory terminal portion and intercalated duct cell of the rat parotid gland were immunoreaction positive.
In bone marrow microenvironment, various cells are expected to interact each other revulating their functions through mediators and cell-contact Then, the effect of TGF-β1, bFGF, TNF-α, IL-4, and LIF on the stem cell factor (SCF) splice variants expression of bone marrow cells were studied by using flow cytometry and quantitative RT-PCR. Flow cytometry analysis demonstrated that the number of cells expressing membrane-bound SCF increased when the cells were treated with TGF-β1 or bFGF. Quantitative RT-PCR analysis demonstrated that TGF-β 1 recuced both SCF-1 and SCF-2 mRNAs synthesis in a dose dependent manner These results suggest that expressions of stem cell factor splice variants in mouse bone marrow cells were regulated individually under co-stimulation of TGF-β1, bFGF, TNF-α, IL-4, and LIF. Less

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report

Research Products

(9 results)

All 2005 Other

All Journal Article (9 results)

  • [Journal Article] エポン切片-イオンエッチング-SEM法の骨組織三次元構造解析への応用。2005

    • Author(s)
      井上庸子
    • Journal Title

      福岡歯大学会雑誌 31・4

      Pages: 185-203

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] エポン切片-イオンエッチング-走査電顕法によるラット舌背後方部糸状乳頭毛細血管ループの三次元微細構造解析。2005

    • Author(s)
      水町栄美理, 長門俊一
    • Journal Title

      解剖誌 80・補遺2

      Pages: 77-78

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Morphological analysis of the three-dimensional structure of bone tissues by the epoxy resin section-ion etching-SEM method.2005

    • Author(s)
      Yoko INOUE
    • Journal Title

      The journal of Fukuoka Dental College. Vol.31, No.4

      Pages: 185-203

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Morphological analysis of the three-dimensional structure of capillary Netwook of the posterior filiform papillae of rat tongue by the epoxy resin Section-ion etching-SEM method.2005

    • Author(s)
      Emiri MIZUMACHI, Toshikazu NAGATO
    • Journal Title

      Acta Anatmica Nipponica Vol.80 Suppl.2

      Pages: 77-78

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Application of ion etching to immunoscanning electron microscopy.2005

    • Author(s)
      Junko YAHIRO, Toshikazu NAGATO
    • Journal Title

      Microscopy Research and technique Vol.67

      Pages: 240-247

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] エポン切片-イオンエッチング-SEM法の骨組織三次元構造解析への応用2005

    • Author(s)
      井上庸子
    • Journal Title

      福岡歯科大学学会雑誌 31・4

      Pages: 185-203

    • Related Report
      2005 Annual Research Report
  • [Journal Article] エポン切片-イオンエッチング-走査電顕法によるラット舌背後方部糸状乳頭毛細血管ループの三次元微細構造解析2005

    • Author(s)
      水町栄美理, 長門俊一
    • Journal Title

      解剖誌 80・補遺2

      Pages: 77-78

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Application of ion etching to immunoscanning electron microscopy.2005

    • Author(s)
      Junko Yahiro, Toshikazu Nagato
    • Journal Title

      Microscopy Research and Technique 67

      Pages: 240-247

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Application of ion etching to immunoscanning electron microscopy.

    • Author(s)
      J.Yahiro, T.Nagato
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-03-31   Modified: 2016-04-21  

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