| Project/Area Number |
16591862
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
INENAGA Kiyotoshi Kyushu Dental College, School of Dentistry, Professor, 歯学部, 教授 (90131903)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Eiko Kyushu Dental College, School of Dentistry, Lecturer, 歯学部, 講師 (00047812)
ONO Kentaro Kyushu Dental College, School of Dentistry, Assistant Professor, 歯学部, 助手 (40316154)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | Dry mouth / Osmotic perception / Sodium sensing / Nax receptor / Drinking / Slice patch / Mouse |
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| Research Abstract |
Thirst sensation is induced by stimulating the thirst center, including the circumventricular organs. The electrical activities were recorded from mouse slice preparation, by using patch clam recordings. SFO neurons were depolarized by hypertonic stimulation, under current clamp mode. The depolarization amplitude in the 30 mM NaC1 stimulation was large than that in the 60 mM mannitol stimulation. The same stimuli induced inward currents under voltage clamp mode. The inward currents were also larger in the NaC1 than mannitol. As for postsynaptic currents, the frequencies of EPSCs were increased and those of IPSCs were decreased by the same hypertonic stimuli. The increment and decrement in synaptic currents were also larger in the NaC1 than mannitol. The amplitude of their synaptic currents was unaffected. There suggest that SFO neurons sense to both hypertonicity and sodium ions and the sodium sensing is superior to the osmotic perception.
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