| Project/Area Number |
16591892
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Pathobiological dentistry/Dental radiology
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
TANUMA Jun-ichi Kagoshima University, Graduate School of Medical and Dental Sciences, Associate professor, 大学院・医歯学総合研究科, 助教授 (20305139)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIRAYAMA Yoshikazu Kagoshima University, Graduate School of Medical and Dental Sciences, Research Associate, 大学院・医歯学総合研究科, 助手 (50343364)
SEMBA Ichiro Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (60145505)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
|---|
| Keywords | Tongue carcinogenesis / QTL analysis / Candidate genes / cDNA Microarray / Protein array |
|---|
| Research Abstract |
Our group has previously used cDNA microarrays to characterize global transcriptional changes. These genes can be broadly categorized based upon their functions in metabolism, angiogenesis, invasion/tissue remodeling, apoptosis, and proliferation/differentiation. Many of these genes contribute to tumor progression and increased malignancy. However, transcriptional changes alone are not sufficient to characterize the complexity of the tumor cell response to hypoxia. Other investigators have reported a poor correlation between mRNA and protein abundance. Furthermore, a single gene can encode for more than one mRNA species through differential splicing, and proteins can undergo as many as 200 posttranslational modifications. These processes all contribute to a large number of different proteins that can be produced from a single gene. Interpretation of genomic and proteomic data are additionally complicated by the fact that we are only able to obtain a static picture of a highly complex, interrelated, and dynamic process.
Currently, there are many competing technologies and approaches to identify carcinogenesis markers reliably for prognostic and therapeutic purposes. The aim of this study was to characterize global changes in the proteome. Using this approach, we have identified a group of hypoxia-regulated proteins that are induced by posttranscriptional mechanisms. These hypoxia-inducible proteins represent novel diagnostic/therapeutic targets. We also investigated the significance of one of these proteins, NQO1 and Pthrp, by correlating tumor carcinogenesis and metastatis with expression of this protein in squamous cell carcinomas of the tongue.
|
