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Antitumor effect of gene transfection and DNA vacccine of beta-defensins

Research Project

Project/Area Number 16591894
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

ABIKO Yoshihiro  Health Sciences University of Hokkaido, Institute of Personalized Medical Science, Professor (90260819)

Co-Investigator(Kenkyū-buntansha) KAKU Tohru  Health Sciences University of Hokkaido, School of Dentistry, Professor (60133253)
ARAKAWA Toshiya  Health Sciences University of Hokkaido, School of Dentistry, Assistant Professor (40306254)
NAGAYASU Hiroki  Health Sciences University of Hokkaido, Institute of Personalized Medical Science, Associate Professor (90265075)
OKUMURA Kazuhiko  Health Sciences University of Hokkaido, School of Dentistry, Assistant Professor (60194510)
Project Period (FY) 2004 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsBeta-defensin / Antitumor / Gene therapy / DNA vaccine / Squamous cell carcinoma / Lymphoma / 樹状細胞 / 遺伝子導入 / CCR6
Research Abstract

The present study examined whether increased expression of beta-defensins by the gene transfection affected antitumor of squamous cell carcinoma (SCC), and whether DNA vaccine with beta-defensins affected antitumor of lymphoma. In order to observe the antitumor effect of increased expression of beta-defensins on SCC, adenovirus vectors with beta-defensin were transfected into SCC tissues in nude mice. The diameter of the tumor mass into which the gene was trandected was significantly smaller than that of the controls. The results indicate that beta-defensins themselves may posses a direct antitumor effect against SCC. In order to observe the antitumor effect of DNA vaccine with beta-defensins on lymphoma, the vaccine was injected into A20 lymphoma inBALB/c mice. The survival rate of the vaccination group was significantly longer than that of the control group. The results indicate that DNA vaccine with beta-defensins may be useful in the treatment of lymphoma.

Report

(5 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (36 results)

All 2007 2006 2005 2004

All Journal Article (36 results) (of which Peer Reviewed: 13 results)

  • [Journal Article] Increased expression of beta-defensin-2 and -3 during the development of autoimmune sialoadenitis in MRL/lpr mice2007

    • Author(s)
      Saitoh M, Kurashige Y, Yamaza ki M, Nishimura M, Nakamura S, Noro D, TakeshimaM, Araka wa T, Takuma T, Igarashi S, Ka ku T, Inoue T, Abiko Y
    • Journal Title

      Med Mol Morph 40

      Pages: 157-162

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Salivary defensins and their importance in oral health and disease(Review)2007

    • Author(s)
      Abiko Y, Saitoh M
    • Journal Title

      Curr Pharm Design 13

      Pages: 3065-3072

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Role of beta-defensin in oral epithelial health and disease(Award review)2007

    • Author(s)
      Abiko Y, Satoh M, Nishimura M, Yamazaki M, Sawamura D, Kaku T
    • Journal Title

      Med Mol Morph 40

      Pages: 179-184

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Increased expression of beta-defensin-2 and -3 during the development of autoimmune sialoadenitis in MRL/lpr mice. Med Mol Morph2007

    • Author(s)
      Saitoh, M., Kurashige, Y., Yamazaki, M., Nishimura, M., Nakamura, S., Noro, D., Takeshima, M., Arakawa, T., Takuma, T., Igarashi, S., Kaku, T., Inoue, T., Abiko, Y
    • Journal Title

      Med Mol Morph 40

      Pages: 157-162

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Salivary defensins and their importance in oral health and disease(Review)2007

    • Author(s)
      Abiko, Y., Saitoh, M
    • Journal Title

      Curr Pharm Design 13

      Pages: 3065-3072

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Role of beta-defensin in oral epithelial health and disease(Award review)2007

    • Author(s)
      Abiko, Y., Satoh, M., Nishimura, M., Yamazaki, M., Sawamura, D., Kaku, T
    • Journal Title

      Med Mol Morph 40

      Pages: 179-184

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Role of beta-defensin in oral epithelial health and disease2007

    • Author(s)
      Abiko Y., et. al.
    • Journal Title

      Med.Mol.Morphol. 40

      Pages: 179-184

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Salivary defensins and their importance in oral health and disease2007

    • Author(s)
      Abiko Y.and Saitoh M.
    • Journal Title

      Curr.Pharm.Des. 18

      Pages: 3065-3072

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased expression of beta-defensin-2 and -3 during the development of autoimmune sialoadenitis in MRL/lpr mice2007

    • Author(s)
      Saitoh M., et. al.
    • Journal Title

      Med.Mo1.Morphol. 40

      Pages: 157-162

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ameloblastic carcinoma ex ameloblastoma : report of a case-possible involvement of CpG island hypermethylation of the p16 gene in malignant transformation.2007

    • Author(s)
      Abiko Y, Nagayasu H, Takeshima M, Yamazaki M, Nishimura M, Kusano K, Kitajo H, Saitoh M, Kawakami T, Chiba I, Kaku T
    • Journal Title

      Oral Surg Oral Med Oral Pathol Oral Radiol Endod 103

      Pages: 72-76

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Morphology of Malassez's epithelial rest-like cells in the cementum : transmission electron microscopy, immunohistochemical, and TdT-mediated dUTP-biotin nick end labeling studies.2006

    • Author(s)
      Suzuki M, Matsuzaka K, Yamada S, Shimono M, Abiko Y, Inoue T
    • Journal Title

      J Periodontal Res 41

      Pages: 280-287

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Connexin 43 transfection in basaloid squamous cell carcinoma cells2006

    • Author(s)
      Shima K, Muramatsu T, Abiko Y, Yamaoka Y, Sasaki H, Shimono M
    • Journal Title

      Oncol Rep 16

      Pages: 285-288

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Single-nucleotide Polymorphism(SNP) in B-Defensin 2 in a Japanese Population and an Effect of - 1029 SNP on Promoter Activity2005

    • Author(s)
      Kusano k, Abiko Y, Nishimura M, Arakawa T, Takeshima M, Fujimoto A, Takuma T, Kaku T
    • Journal Title

      Oral Science International 2

      Pages: 80-84

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Beta defensin-3 engineered epidermis shows highly protective effect for bacterial infection2005

    • Author(s)
      Sawamura D, Goto M, Shibaki A, Akiyama M, McMillan JR, A biko Y, Shimizu H
    • Journal Title

      Gene Ther 12

      Pages: 857-861

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Single-nucleotide Polymorphism(SNP) in 6-Defensin 2 in a Japanese Population and an Effect of - 1029 SNP on Promoter Activity2005

    • Author(s)
      Kusano, k., Abiko, Y., Nishimura, M., Arakawa, T., Takeshima, M., Fujimoto, A., Takuma, T., Kaku, T
    • Journal Title

      Oral Science International 2

      Pages: 80-84

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Beta defensin-3 engineered epidermis shows highly protective effect for bacterial infection2005

    • Author(s)
      Sawamura, D., Goto, M., Shibaki, A., Akiyama, M., McMillan, JR., Abiko, Y., Shimizu, H
    • Journal Title

      Gene Ther 12

      Pages: 857-61

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Inhibition of osteopontin expression and fenction in oral cancer cell lines by antisense oligonucleotides2005

    • Author(s)
      Muramatsu T., Shima K., Ohta K., Kizaki H., Abiko Y., Shimono M.
    • Journal Title

      Cancer Letter 217

      Pages: 87-95

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Single-nucleotide polymorphism(SNP) in beta-difensin 2 in a Japanese population and an effect of -1029 SNP on promoter activity2005

    • Author(s)
      Kusano K, Abiko Y, Nishimura M, Arakawa T, Takeshima M, Fujimoto A, Takuma T.Kaku T.
    • Journal Title

      Oral Science International 2(2)

      Pages: 80-84

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 成長期ラット顎関飾円板におけるversican isoform mRNAの発現-TaqMan probeを用いたreal-time quantitative PCRによる分析-2005

    • Author(s)
      鳥谷奈保子, 荒川陵哉, 田隈泰信, 安彦善裕, 溝口 到
    • Journal Title

      北海道医療大学歯学会雑誌 24(1)

      Pages: 31-39

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Beta defensin-s engineered epidermis shows highly protective effect fro bacterial infection2005

    • Author(s)
      Sawamura D, Goto M, Shibaki A, Akiyama M, McMillan JR, Abiko Y, Shimizu H.
    • Journal Title

      Gene Therapy 12

      Pages: 857-861

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cell proliferation, apoptosis and apoptosis-related factors in odontogenic keratocysts and in dentigerous cysts2005

    • Author(s)
      Kichii E, Enokiya Y, Muramatsu T, Hashimoto S, Inoue T, Abiko Y, Shimono M.
    • Journal Title

      J Oral Pathol Med 36

      Pages: 280-286

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Current dental implant research2005

    • Author(s)
      Inoue T, Matsuzaka K, Yoshinari M, Tanaka T, Abiko Y., Shimono M
    • Journal Title

      Dentistry in Japan 41

      Pages: 196-213

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Beta defensinn-3 engineered epidermis shows highly protective effect for bacterial infection2005

    • Author(s)
      Sawamura D, Goto M, Shibaki A, Akiyama M, McMillan JR, Abiko Y, et al.
    • Journal Title

      Gene Ther 24

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inhibition of osteopontin expression and function in oral cancer lines by antisense oligonucleotides.2005

    • Author(s)
      Muramatsu T, Shima K, Ohta K, Kizaki H, Ro Y, Kohno Y, Abiko Y, et al.
    • Journal Title

      Cancer Lett 217(1)

      Pages: 87-95

    • Related Report
      2004 Annual Research Report
  • [Journal Article] C-terminal domain of human CAP18 antimicrobial peptide induces apotosis in oral squamous cell2004

    • Author(s)
      Okumura K, Itoh A, Isogai E, Hirose K, Hosokawa Y, Abiko Y, Shibata T, Hirata M, Isogai H
    • Journal Title

      Cancer Lett 212

      Pages: 185-194

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Correlated expression of human beta defensin-1,-2 and -3 mRNAs in gingival tissues of young children2004

    • Author(s)
      Saitoh M, Abiko Y, Shimabukuro S, Kusano K, Nishimura M, Arakawa T, Nakashima K, Takuma T, Kaku T, Igarashi S
    • Journal Title

      Arch Oral Biol 49

      Pages: 799-803

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of defensin peptides on eukaryotic cells primary epithelial cells,flibroblasts and squamous cell carcinoma cell lines2004

    • Author(s)
      Nishimura M, Abiko Y, Kurashi ge Y, Takeshima M, Yamazaki M, Kusano K, Saitoh M, Nakas hima K, Inoue T, Kaku T
    • Journal Title

      J Dermatol Sci 36

      Pages: 87-95

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 小児歯肉におけるヒトβディフェンシン1-2-3mRNAの発現及び局在2004

    • Author(s)
      斉藤 正人、島袋 鎮太郎、坂口 也子、桜井 有子、野呂 大輔、菅谷 裕行、五十嵐 清治、西村 学子、安彦 善裕
    • Journal Title

      小児歯科学雑誌 42

      Pages: 69-74

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 口腔扁平苔癬におけるβディフェンシン2の発現と臨床病理学的所見との関連についての検討2004

    • Author(s)
      野口 忠秀, 神部 芳則, 草間 幹夫, 安彦 善裕, 賀来 亨
    • Journal Title

      日本口腔粘膜学会雑誌 10

      Pages: 6-10

    • NAID

      10013391951

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] C-terminal domain of human CAP18 antimicrobial peptide induces apotosis in oral squamous cell2004

    • Author(s)
      Okumura, K., Itoh, A., Isogai, E., Hirose, K., Hosokawa, Y., Abiko, Y., Shibata, T., Hirata, M., Isogai, H
    • Journal Title

      Cancer Lett 212

      Pages: 185-94

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Correlated expression of human beta defensin-1, -2 and -3 mRNAs in gingival tissues of young children2004

    • Author(s)
      Saitoh, M., Abiko, Y., Shimabukuro, S., Kusano, K., Nishimura, M., Arakawa, T., Nakashima, K., Takuma, T., Kaku, T., Igarashi, S
    • Journal Title

      Arch Oral Biol 49

      Pages: 799-803

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effect of defensin peptides on eukaryotic cells : primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines2004

    • Author(s)
      Nishimura, M., Abiko, Y., Kurashige, Y., Takeshima, M., Yamazaki, M., Kusano, K., Saitoh, M., Nakashima, K., Inoue, T., Kaku, T
    • Journal Title

      J Dermatol Sci 36

      Pages: 87-95

    • NAID

      10016185292

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] An immunohistochemical study of the localization of induviblee nitric oxide synthase(iNOS) and heat shock protein(HSP) in pleomorphic adenoma2004

    • Author(s)
      Yamaguchi M, Abiko Y, Nishimura M, et al.
    • Journal Title

      Acta Histochem.Cytochem. 37(4)

      Pages: 267-271

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effect of defensin peptides on eukaryotic cells : primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines2004

    • Author(s)
      Nishimura M, Abiko Y, et al.
    • Journal Title

      J.Dermatol Sci. 36(2)

      Pages: 87-95

    • NAID

      10016185292

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Correlated expression of human beta defensin-1,-2 and -3 mRNAs in gingival tissues of young children2004

    • Author(s)
      Saitoh M, Abiko Y, Shimabukuro S, Kusano K, Nishimura M, et al.
    • Journal Title

      Arch Oral Biol 49

      Pages: 499-803

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Atypical carcinoid (neuroendocrine carcinoma) of the gingival : Couterpart of a laryngeal tumor2004

    • Author(s)
      Abiko Y, Ogawa I, Hattori Y, Kusano K, Nishimura M, et al.
    • Journal Title

      Pathol.International 54(2)

      Pages: 97-100

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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