| Project/Area Number |
16591894
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
ABIKO Yoshihiro Health Sciences University of Hokkaido, Institute of Personalized Medical Science, Professor (90260819)
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| Co-Investigator(Kenkyū-buntansha) |
KAKU Tohru Health Sciences University of Hokkaido, School of Dentistry, Professor (60133253)
ARAKAWA Toshiya Health Sciences University of Hokkaido, School of Dentistry, Assistant Professor (40306254)
NAGAYASU Hiroki Health Sciences University of Hokkaido, Institute of Personalized Medical Science, Associate Professor (90265075)
OKUMURA Kazuhiko Health Sciences University of Hokkaido, School of Dentistry, Assistant Professor (60194510)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Beta-defensin / Antitumor / Gene therapy / DNA vaccine / Squamous cell carcinoma / Lymphoma / 樹状細胞 / 遺伝子導入 / CCR6 |
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| Research Abstract |
The present study examined whether increased expression of beta-defensins by the gene transfection affected antitumor of squamous cell carcinoma (SCC), and whether DNA vaccine with beta-defensins affected antitumor of lymphoma. In order to observe the antitumor effect of increased expression of beta-defensins on SCC, adenovirus vectors with beta-defensin were transfected into SCC tissues in nude mice. The diameter of the tumor mass into which the gene was trandected was significantly smaller than that of the controls. The results indicate that beta-defensins themselves may posses a direct antitumor effect against SCC. In order to observe the antitumor effect of DNA vaccine with beta-defensins on lymphoma, the vaccine was injected into A20 lymphoma inBALB/c mice. The survival rate of the vaccination group was significantly longer than that of the control group. The results indicate that DNA vaccine with beta-defensins may be useful in the treatment of lymphoma.
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