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A Role of HMGIC in Squamous Carcinoma Cells of The Oral Cavity

Research Project

Project/Area Number 16591898
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionThe Nippon Dental University

Principal Investigator

IMAI Kazushi  The Nippon Dental University, Department of biochemistry, Associate Professor, 歯学部, 助教授 (10328859)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsOral carcinomas / HMGA2 / Transcription factor / sChIP / SChIP
Research Abstract

In a previous study, we showed that expression of a transcription factor-high mobility group protein (HMGIC/HMGA2), which plays a critical role in differentiation and growth of un-differentiated mesenchymal cells during development, is frequently observed in oral carcinomas and associates with 5-year survival rate of patients suffered from the disease. Understanding a role of HMGIC expression should expand knowledge of a molecular mechanism(s) of cancer progression. In this end, we developed a novel high-throughput analysis, named sChIP, to unveil HMGA2 target genes. We identified approx. 100 genes, and focused on 5 different genes, which are expected to be involved in the progression of oral carcinomas. RT-PCR showed that expression of these 5 genes were negligible in differentiated HaCaT keratinocytes but upregulated in undifferentiated HaCaT cells. In addition, oral carcinoma cells without HMGIC expression (KOSC3, Ho1u1) did not express these 5 genes in contrast to the expression in carcinoma cells expressing HMGIC gene (HOC313, TSU). To understand the transcriptional activity of HMGIC, we are currently investigating it by a reporter assay using luciferase system. Expression of HMGIC target genes in keratinocytes isolated from Hmgic-null mice is also under investigation. We will develop carcinoma cell lines which are specifically knock-downed HMGA2 gene by RNAi, and analyze a direct role of HMGIC and the target genes on tumorgenisity, and invasiveness, metastatic potential and phenotypic alterations of carcinoma cells.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (16 results)

All 2005 2004

All Journal Article (13 results) Book (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Ectopic activation of mesenchyme-specific genes induce the epithelial-mesenchymal transition in oral cancer.2005

    • Author(s)
      Kazushi Imai
    • Journal Title

      Res. Adv. in Cancer 5

      Pages: 41-47

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression and regulation of WISP2 in rheumatoid arthritic synovium.2005

    • Author(s)
      Ichiro Tanaka
    • Journal Title

      Biochem. Biophys. Res. Commun. 334

      Pages: 973-978

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differential expression and localization of WNTs in an animal model of skin wound healing.2005

    • Author(s)
      Toshiyuki Okuse
    • Journal Title

      Wound Repair Regen. 13

      Pages: 491-497

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Expression of SIP1 in oral squamous cell carcinomas : implications for E-cadherin expression and tumor progression.2005

    • Author(s)
      Genta Maeda
    • Journal Title

      Int. J. Oneol. 27

      Pages: 1535-1541

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ectopic activation of mesenchyme-specific genes induce the epithelial-mesenchymal transition in oral carcinomas.2005

    • Author(s)
      Kazushi Imai, et al.
    • Journal Title

      Res.Adv.in Cancer 5

      Pages: 41-47

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression and regulation of WISP2 in rheumatoid arthritic synovium.2005

    • Author(s)
      Ichiro Tanaka, et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 334

      Pages: 973-978

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differential expression and localization of WNTs in an animal model of skin wound healing.2005

    • Author(s)
      Toshiyuki Okuse, et al.
    • Journal Title

      Wound Repair Regen. 13

      Pages: 491-497

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of SIP1 in oral squamous cell carcinomas : implications for E-cadherin expression and tumor progression.2005

    • Author(s)
      Genta Maeda, et al.
    • Journal Title

      Int.J.Oncol. 27

      Pages: 1535-1541

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ectopic activation of mesenchyme-specific genes induce the epithelial mesenchymal transition in oral cancer.2005

    • Author(s)
      Kazushi Imai
    • Journal Title

      Res.Adv.in Cancer 5

      Pages: 41-47

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression and regulation of WISP2 in rheumatoid arthritic synovium.2005

    • Author(s)
      Ichiro Tanaka
    • Journal Title

      Biochem.Biophys.Res.Commun. 334

      Pages: 973-978

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of SIP1 in oral squamous cell carcinomas : implications for E-cadherin expression and tumor progression.2005

    • Author(s)
      Genta Maeda
    • Journal Title

      Int.J.Oncol. 27

      Pages: 1535-1541

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Epithelial-mesenchymal transition and progression of oral carcinomas.2004

    • Author(s)
      Kazushi Imai
    • Journal Title

      Gene Ther. Mol. Biol. 8

      Pages: 195-200

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Epithelial-mesenchymal transition and progression of oral carcinomas.2004

    • Author(s)
      Kazushi Imai
    • Journal Title

      Gene Ther.Mol.Biol. 8

      Pages: 195-200

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Book] Ectopic activation of mesenchyme-specific genes induce the epithelial-mesenchymal transition in oral cancer, in "Research Advances in Cancer"2005

    • Author(s)
      Kazushi Imai
    • Publisher
      Global Research Network(未定)
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 転写因子の標的遺伝子検出方法2005

    • Inventor(s)
      今井一志, 岡崎真大
    • Industrial Property Rights Holder
      今井一志
    • Industrial Property Number
      2005-029285
    • Filing Date
      2005-02-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 転写因子の標的遺伝子検出方法2005

    • Inventor(s)
      今井 一志, 岡崎 真大
    • Industrial Property Rights Holder
      今井 一志
    • Industrial Property Number
      2005-029285
    • Filing Date
      2005-02-04
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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