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Establishment of diagnosis of apical periodontitis utilizing the microbiological and immunological profiling of root canals

Research Project

Project/Area Number 16591905
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionTohoku University

Principal Investigator

YAMAKI Keiko  Tohoku University, Grad Sch of Dentistry, Assistant, 大学院歯学研究科, 助手 (90182419)

Co-Investigator(Kenkyū-buntansha) SHIMAUCHI Hidetoshi  Tohoku University, Grad Sch of Dentistry, Professor, 大学院歯学研究科, 教授 (70187425)
SATO Takuichi  Tohoku University, Grad Sch of Dentistry, Lecturer, 大学院歯学研究科, 講師 (10303132)
ENDO Hideaki  Tohoku University, Grad Sch of Dentistry, Assistant, 大学院歯学研究科, 助手 (80168830)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsApical periodontitis / PCR / Exudate in root canals / 16S ribosomal RNA
Research Abstract

This study aimed to profile microflora of root canals before and after root canal therapy, utilizing real-time PCR and cloning-sequence analyses based on 16S rRNA genes. Informed consent was obtained from 5 patients, and 6 single-rooted teeth with periapical lesions were investigated. Upon access opening, dentin sample was collected from the root canal. When the periapical lesion healed clinically through chemo-mechanical cleaning and intracanal medication, the canal dentin was sampled again. The quantification of total bacteria was performed by real-time PCR using universal primers based on 16S rRNA genes. PCR products were cloned and partially sequenced, and bacterial identification was carried out by comparative analysis with the GenBank database. The concentrations of bacterial DNA were 0.36-152.0 (52.7±54.1) ng/mL upon access opening, and lowered to 0.02-36.00 (10.9±16.6) ng/mL after root canal therapy. The cloning-sequence analysis revealed that although Fusobacterium were initially predominant, Pseudomonas, Bradyrhizobium and Methylobacterium prevailed after root canal therapy. These results suggested that changes in bacterial flora brought by root canal therapy were not only in its quantity but also in quality, and that the dramatic shift in its components might also contribute to the healing process.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (21 results)

All 2007 2006 2005 2004

All Journal Article (21 results)

  • [Journal Article] Profiling of bacterial flora in crevices around titanium orthodontic anchor plates2007

    • Author(s)
      Sato R
    • Journal Title

      Clin Oral Implant Res 18(1)

      Pages: 21-26

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Microflora profiling of root canal utilizing real-time PCR and cloning-sequence analyses based on 16S rRNA genes-Differences between before and after root canal treatments-2007

    • Author(s)
      Ito Y
    • Journal Title

      International Congress Series (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of orally administered Lactobacillus salivarius WB21 supplement on periodontal clinical parameters and microflora2007

    • Author(s)
      Mayanagi G
    • Journal Title

      International Congress Series (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Profiling of bacterial flora in crevices around titanium orthodontic anchor plates.2007

    • Author(s)
      Sato R
    • Journal Title

      Clin Oral Implant Res 18(1)

      Pages: 21-26

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Microflora profiling of root canal utilizing real-time PCR and cloning-sequence analyses based on 16S rRNA genes -Differences between before and after root canal treatments-.Q2007

    • Author(s)
      Ito Y
    • Journal Title

      International Congress Series (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of orally administered Lactobacillus salivarius WB21 supplement on periodontal clinical parameters and microflora.2007

    • Author(s)
      Mayanagi G
    • Journal Title

      International Congress Series (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Microflora profiling of root canal utilizing real-time PCR and cloning-sequence analyses based on 16SrRNA genes-Differences between before and after root canal treatments-.2007

    • Author(s)
      Ito Y
    • Journal Title

      International Congress Series (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comparison of age-dependent expression of aggrecan and ADAMTSs in mandibular condylar cartilage, tibial growth plate, and articular cartilage in rats.2006

    • Author(s)
      Mitani H
    • Journal Title

      Histochem Cell Biol 126(3)

      Pages: 371-380

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Profiling of bacterial flora in crevices around titanium orthodontic anchor plates2006

    • Author(s)
      Sato R
    • Journal Title

      Clin Oral Implant Res 17(in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] PCR for detection of mutans streptococci in human dental plaque2005

    • Author(s)
      Matsuyama J
    • Journal Title

      International Congress Series 1284

      Pages: 158-162

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Hydrogen sulfide-producing bacteria in tongue coating and their relationship with oral malodour2005

    • Author(s)
      Washio J
    • Journal Title

      J Med Microbiol 54(9)

      Pages: 889-895

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Quantification and detection of bacteria from postoperative maxillary cyst by polymerase chain reaction2005

    • Author(s)
      Yamaura M
    • Journal Title

      Oral Microbiol Immunol 20(6)

      Pages: 333-338

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] PCR for detection of mutans streptococci in human dental plaque.2005

    • Author(s)
      Matsuyama J
    • Journal Title

      International Congress Series 1284

      Pages: 158-162

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Hydrogen sulfide-producing bacteria in tongue coating and their relationship with oral malodour.2005

    • Author(s)
      Washio J
    • Journal Title

      J Med Microbiol 54(9)

      Pages: 889-895

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Quantification and detection of bacteria from postoperative maxillary cyst by polymerase chain reaction.2005

    • Author(s)
      Yamaura M
    • Journal Title

      Oral Microbiol Immunol 20(6)

      Pages: 333-338

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Detection frequency of periodontitis-associated bacteria by polymerase chain reaction in subgingival and supragingival plaque of periodontitis and healthy subjects.2004

    • Author(s)
      Mayanagi G
    • Journal Title

      Oral Microbiol Immunol 19(6)

      Pages: 379-385

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A method for mapping the distribution pattern of cariogenic streptococci within dental plaque in vivo.2004

    • Author(s)
      Kato K
    • Journal Title

      Caries Res 38(5)

      Pages: 448-453

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Porphyromonas gingivalis lipopolysaccharides induce maturation of dendritic cells with CD14+CD16+ phenotype.2004

    • Author(s)
      Kanaya S
    • Journal Title

      Eur J Immunol 34(5)

      Pages: 1451-1460

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The involvement of platelet-derived growth factor receptors and insulin-like growth factor-I receptors signaling during mineralized nodule formation by human periodontal ligament cells.2004

    • Author(s)
      Nemoto E
    • Journal Title

      J Periodontal Res 39(6)

      Pages: 388-397

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of CD73/ecto-5'-nucleotidase on human gingival fibroblasts and contribution to the inhibition of interleukin-1alpha-induced granulocyte-macrophage colony stimulating factor production.2004

    • Author(s)
      Nemoto E
    • Journal Title

      J Periodontal Res 39(1)

      Pages: 10-19

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Endogenous IL-15 sustains recruitment of IL-2Rbeta and common gamma and IL-2-mediated chemokine production in normal and inflamed human gingival fibroblasts.2004

    • Author(s)
      Ozawa A
    • Journal Title

      J Immunol 173(8)

      Pages: 5180-5188

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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