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Development of a risk assessment method for dental materials using a bone metabolic system

Research Project

Project/Area Number 16591913
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionHiroshima University

Principal Investigator

TANAKA Nobuyuki  Hiroshima University, Hospital, Lecturer, 病院, 講師 (30253090)

Co-Investigator(Kenkyū-buntansha) SHIRAI Kenichi  Hiroshima University, Graduate School of Biomedical Sciences, Research Associate, 大学院医歯薬学総合研究科, 助手 (20325179)
ARAKAWA Makoto  Hiroshima University, Hospital, Research Associate, 病院・助手 (60379881)
NOMURA Yuji  Hiroshima University, Graduate School of Biomedical Sciences, Research Associate, 大学院医歯薬学総合研究科, 助手 (80218370)
OKAZAKI Masayuki  Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院医歯薬学総合研究科, 教授 (30107073)
SAWAJIRI Masahiko  Hiroshima University, Hospital, Research Associate, 病院・助手 (20325195)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsDental materials / Risk assessment / Genotoxicity / Bone metabolic system / Estrogenic activity / 安全性評価方法
Research Abstract

Many chemicals released into the environment are believed to disrupt normal endocrine functions in humans and anima1s. These endocrine-disrupting chemicals (EDCs) affect reproductive health and development. Currently, attention is focused on the development of in vivo and in vitro screening strategies to identify and classify EDCs, in order to determine whether such chemicals pose a hazard to human health. In previous studies, we reported on the estrogenic activity of compounds, receptor-binding assays, and receptor gene expression assays using cultured cells and yeast cells, and a yeast two-hybrid assay. These screening tests were applied to the risk evaluation of dental materials. Despite the usefulness of these methods for detecting estrogenicity, they have their limitations. In particular, very little is known about whether EDCs affect bone formation. This study investigated the effects of the genotoxicity and estrogenicity of dental materials. It is hoped that a bioassay of EDCs and genotoxic chemicals in vivo can be established, using osteoblasts, and then applied, to judge the safety of the chemicals that leach from dental materials in the mouth. Summarizing the results of the various analyses carried out in these studies, the following conclusions were drawn:
1) Chemicals such as resin monomers and plasticizers leach out of dental resins after polymerization.
2) Some components of dental materials have genotoxic or estrogenic activity.
3) Measurements of RANKL, OPG, ERα, and ERβ mRNA in osteoblasts exposed to chemicals were useful for evaluating risk assessment in a bone metabolic system.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (28 results)

All 2007 2006 2005 2004 Other

All Journal Article (28 results)

  • [Journal Article] High-risk human papillomavirus type 16 E7 oncogene associates with Cdc25A over-expression in oral squamous cell carcinoma2007

    • Author(s)
      U.K.Bhawal
    • Journal Title

      Virchows Archiv 450

      Pages: 65-71

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] UCN-01 (7-hydroxystaurosporine) induces apoptosis and G1 arrest of both primary and metastatic oral cancer cell lines in vitro2007

    • Author(s)
      A.Otsubo
    • Journal Title

      Oral Surgery, Oral Medicine, Oral pathology, Oral Radiology and Endoontics 103(3)

      Pages: 391-397

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of MMP-13 in osteoblast cells and rat tibia after exposure to gamma rays or accelerated carbon ions2007

    • Author(s)
      M.Sawajiri
    • Journal Title

      Physica Medica (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] High-risk human papillomavirus type 16 E7 oncogene associates with Cdc25A over-expression in oral squamous cell carcinoma.2007

    • Author(s)
      U.K.Bhawal
    • Journal Title

      Virchows Archiv 450

      Pages: 65-71

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] UCN-01 (7-hydroxystaurosporine) induces apoptosis and G1 arrest of both primary and metastatic oral cancer cell lines in vitro.2007

    • Author(s)
      A.Otsubo
    • Journal Title

      Oral Surgery, Oral Medicine, Oral pathology, Oral Radiology and Endoontics 103(3)

      Pages: 391-397

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] High-risk human papillomavirus type 16 E7 oncogene associates with Cdc25A over expression in oral squamous cell carcinoma2007

    • Author(s)
      U.K.Bhawal
    • Journal Title

      Virchows Archiv 450

      Pages: 65-71

    • Related Report
      2006 Annual Research Report
  • [Journal Article] UCN-01(7-hydroxystaurosporine)induces apoptosis and G1 arrest of both primary and metastatic oral cancer cell lines in vitro2007

    • Author(s)
      A.Otsubo
    • Journal Title

      Oral Surgery, Oral Medicine, Oral pathology, Oral Radiology and Endoontics 103(3)

      Pages: 391-7

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Genotoxicity of dental resin polymerization initiators in vitro2006

    • Author(s)
      Y.Nomura
    • Journal Title

      Journal of Materials Science : Materials in Medicine 17(1)

      Pages: 29-32

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Estrogenic activity of phthalate esters by in vitro VTG assay using primary cultured Xenopus hepatocytes2006

    • Author(s)
      Y.Nomura
    • Journal Title

      Dental Materials Journal 25(3)

      Pages: 618-624

    • NAID

      110004810977

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Different effects of carbon ion and γ irradiation on expression of receptor activator of NF-kB ligand in MG3T3-E1 osteoblast cells2006

    • Author(s)
      M.Sawajiri
    • Journal Title

      Bulletin of Experimental Biology and Medicine 142(5)

      Pages: 566-572

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genotoxicity of dental resin polymerization initiators in vitro.2006

    • Author(s)
      Y.Nomura
    • Journal Title

      Journal of Materials Science Materials in Medicine 17(1)

      Pages: 29-32

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Estrogenic activity of phthalate esters by in vitro VTG assay using primary-cultured Xenopus hepatocytes.2006

    • Author(s)
      Y.Nomura
    • Journal Title

      Dental Materials Journal 25(3)

      Pages: 618-624

    • NAID

      110004810977

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Different effects of carbon ion and y -irradiation on expression of receptor activator of NF-kB ligand in MC3T3-E1 osteoblast cells.2006

    • Author(s)
      M.Sawajiri
    • Journal Title

      Bulletin of Experimental Biology and Medicine 142(5)

      Pages: 566-572

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Genetoxicity of dental resin polymerization initiators in vitro2006

    • Author(s)
      Y.Nomura
    • Journal Title

      Materials Science : Materials in Medicine. 17(1)

      Pages: 29-32

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Estrogenic activity of phthalate esters by in vitro VTG assay using primary-cultured Xenopus hepatocytes2006

    • Author(s)
      Y.Nomura
    • Journal Title

      Dental Materials Journal 25(3)

      Pages: 533-7

    • NAID

      110004810977

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Gene Expression of Receptor activator of NF-κB ligand in MC3T3-E1 Osteoblast Cells after Carbon-ion and Gamma-ray Irradiation2006

    • Author(s)
      M.Sawajiri
    • Journal Title

      Bulletin of Experimental Biology and Medicine 11

      Pages: 566-72

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Genotoxicity of dental resin polymerization initiators in vitro2006

    • Author(s)
      Nomura Y, Teshima W, Kawahara T, Tanaka N, Ishibashi H, Okazaki M, Arizono K.
    • Journal Title

      Journal of Materials Science : Materials in Medicine 17(1)

      Pages: 29-32

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Temporary restorative resins using non-phthalate ester plasticizers2005

    • Author(s)
      N.Tanaka
    • Journal Title

      Journal of Dentistry. 33(7)

      Pages: 577-583

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] SEM observation of collagen fibrils secreted from the body surface of osteoblasts on a CO3apatite-collagen sponge2005

    • Author(s)
      I.Hirata
    • Journal Title

      Dental Materials Journal 24(3)

      Pages: 460-464

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Temporary restorative resins using non・phthalate ester plasticizers2005

    • Author(s)
      N.Tanaka
    • Journal Title

      Journal of Dentistry 33(7)

      Pages: 577-583

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] SEM observation of collagen fibrils secreted from the body surface of osteoblasts on a CO3apatite-collagen sponge.2005

    • Author(s)
      I.Hirata
    • Journal Title

      Dental Materials Journal 24(3)

      Pages: 460-464

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Temporary restorative resins using non-phthalate ester plasticizers2005

    • Author(s)
      N.Tanaka, Y.Nomura, R.Nishikiori, S.Shibata, K.Shirai, M.Fujitani
    • Journal Title

      Journal of Dentistry 33(7)

      Pages: 577-583

    • Related Report
      2005 Annual Research Report
  • [Journal Article] SEM Observation of Collagen-Fibrils secreted from the Body Surface of Osteoblasts on a CO_3apatite-collagen Sponge2005

    • Author(s)
      I.HIRATA, Y.NOMURA, H.TABATA, Y.MIAKE, T.YANAGISAWA, M.OKAZAKI
    • Journal Title

      Dental Materials Journal 24(3)

      Pages: 460-464

    • NAID

      110002983730

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Association of Expression of Receptor for Advanced Glycation End Products and Invasive Activity of Oral Squamous Cell Carcinoma2005

    • Author(s)
      Bhawal UK, Ozaki Y, Nishimura M, Sugiyama M, Sasahira T, Nomura Y, Sato F, Fujimoto K, Sasaki N, Ikeda MA, Tsuji K, Kuniyasu H, Kato Y.
    • Journal Title

      Oncology 69(3)

      Pages: 246-255

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Leachability of plasticizer and residual monomer from commercial temporary restorative resins.2004

    • Author(s)
      T.Kawahara
    • Journal Title

      Journal of Dentistry. 32(4)

      Pages: 277-283

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Leachability of plasticizer and residual monomer from commercial temporary restorative resins.2004

    • Author(s)
      T.Kawahara
    • Journal Title

      Journal of Dentistry 32(4)

      Pages: 277-283

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Temporary restorative resins using non-phthalate ester plasticizers

    • Author(s)
      N.Tanaka, Y.Nomura, et al.
    • Journal Title

      Journal of Dentistry (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genotoxicity of dental resin polymerization initiators in vitro

    • Author(s)
      Y.Nomura, M.Okazaki, et al.
    • Journal Title

      Journal of Materials Science : Materials in Medicine (In press)

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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