| Project/Area Number |
16591921
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Meikai University (2007)
Showa University (2004-2006) |
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Principal Investigator |
NAKAMURA Yukio Meikai University, School of Dentistry, Professor (40207931)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Haruji Showa University, School of Dentistry, Assistant Professor (90191783)
MASUDA Yoshiko Showa University, School of Dentistry, Assistant Professor (10297038)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,660,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥60,000)
Fiscal Year 2007: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | ameloblastin / pulp / dentin / wound healing / immunohistochemistry / Enamel Matrix Proteins (EMD) / type I Collagen / DSP / shenthlin |
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| Research Abstract |
We have shown that enamel matrix derivative (EMD) enhanced reparative dentin formation was associated with a sequential expression of dentin-specific factors in the wounded pulp. Notably, in these studies, expression of pig ameloblastin was detected in pulpal cells in the wound area shortly following EMD application. To confirm a functional role for Ambn in dentinogenesis and to explore the potential use of Ambn for pulpal healing, we investigated if application of recombinant fusion Ambn (rAmbn) onto exposed pulp tissue could enhance pulpal healing and secondary dentin formation by histomorphometry and immunohistochemistry. In rAmbn-treated teeth, a substantial amount of newly formed reparative dentin was observed at the application site, completely bridging the pulpal wound. Dentin formation was also observed in Ca(OH)2 trated teeth; however, the amount of reparative dentin was significantly smaller than after rAmbn treatment. Immunohistochemistry confirmed that the new hard tissue formed was similar to dentin. The results suggest potential for rAmbn as a biologically active pulp-dressing agent for enhanced pulpal wound healing and reparative dentin formation after pulpotomy procedures.
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