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Molecular histological analysis on calcification of alveolar bone during healing process of chronic periapical lesion.

Research Project

Project/Area Number 16591922
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionShowa University

Principal Investigator

KIMURA Yuichi  Showa University, Associate Professor, 歯学部, 助教授 (60211877)

Co-Investigator(Kenkyū-buntansha) YAMADA Yoshishige  Showa University, Assistant Professor, 歯学部, 講師 (40360127)
KINOSHITA Jun-Ichiro  Showa University, Assistant Professor, 歯学部, 講師 (90360122)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsApical lesion / Rats / Histological analysis / Calcification / Laser / Root canal preparation / Root canal medication / Healing process / 歯内治療 / 石灰化 / 熱的影響 / 歯根吸収
Research Abstract

The effects of Er : YAG laser irradiation and root canal medication on the periapical lesion during root canal treatment were evaluated using Wistar rats (8 weeks old and weighing 200-300g) histopathologically. After root canals were left unsealed for 1 month, root canals were irradiated using an Er:YAG laser at 2 Hz and 34, 68, and 102 mJ/pulse for 30 seconds. Non-irradiated canals prepared by a conventional method of Grossman served as controls. After calcium hydroxide was put into root canals as medication and root canals were sealed with composite resin, the effects of laser irradiation on the periodontal tissues at apical area were evaluated at 0, 2 days, 1, 2, and 4 weeks after irradiation under light microscopy after H・E, TRAP, and ALP staining. At 4 weeks after treatment, no inflammation or resorption was observed in any cases in the control or 34 mJ/pulse-irradiated groups. There was no significant difference of healing process between 2 groups. However, moderate to severe inflammation with resorption of root surface was observed in 4 of 5 cases (80%) in the 102 mJ/pulse-irradiated group, which was significantly different from the control (P<0.01). On the other hand, there was no significant difference of healing process among some intracanal medicaments. These results suggest that thermal effects on periapical tissues during root canal preparation by Er:YAG laser irradiation at infected root canals are minimal, if appropriate parameters are selected. Observation period of longer than 4 weeks will be needed in further research. A role of osteopontin as a marker on calcification process and any medicaments to accelerate calcification will be investigated.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (25 results)

All 2007 2006 2005 2004

All Journal Article (25 results)

  • [Journal Article] Effects of KTP laser irradiation, diode laser, and LED on tooth bleaching : a comparative study.2007

    • Author(s)
      Zhang C, Wang X, Kinoshita J-I, Zhao B, Toko T, Kimura Y, Matsumoto K
    • Journal Title

      Photomedicine and Laser Surgery 25・2(In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of KTP laser irradiation, diode laser, and LED on tooth bleaching : a comparative study.2007

    • Author(s)
      Zhang C, Wang X, Kinoshita J-I, Zhao B, Toko T, Kimura Y, Matsumoto K.
    • Journal Title

      Photomed Laser Surg 25

      Pages: 91-95

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of KTP laser irradiation, diode laser, and LED on tooth bleaching : a comparative study.2007

    • Author(s)
      Zhang C, Wang X, Kinoshita J-I, Zhao B, Toko T, Kimura Y, Matsumoto K
    • Journal Title

      Photomedicine and Laser Surgery 25.2(In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Caries removal with Carisolv system : criteria evaluation and microleakage test.2006

    • Author(s)
      Yamada Y, Kimura Y, Hossain M, Kinoshita J-I, Shimizu Y, Matsumoto K
    • Journal Title

      Journal of Clinical Pediatric Dentistry 30・2

      Pages: 121-126

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Morphological and compositional changes in human teeth following 9.6-μm CO_2 laser irradiation in vitro2006

    • Author(s)
      Nagai K, Kinoshita J-I, Kimura Y, Matsumoto K
    • Journal Title

      Journal of Oral Laser Application 6・4

      Pages: 265-276

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Morphological and compositional changes in human teeth following 9.6 μm CO_2 laser irradiation in vitro.2006

    • Author(s)
      Nagai K, Kinoshita J-I, Kimura Y, Matsumoto K.
    • Journal Title

      J Oral Laser Appli 6

      Pages: 265-276

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Morphological and compositional changes in human teeth following 9.6-μm CO_2 laser irradiation in vitro2006

    • Author(s)
      Nagai K, Kinoshita J-I, Kimura Y, Matsumoto K
    • Journal Title

      Journal of Oral Laser Application 6.4

      Pages: 265-276

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Morphological comparative study on ablation of dental hard tissues at cavity preparation by Er : YAG and Er,Cr : YSGG lasers.2005

    • Author(s)
      Harashima T, Kinoshita J-I, Kimura Y, Brugnera A, Zanin F, Pecora JD, Matsumoto K
    • Journal Title

      Photomedicine and Laser Surgery 23・1

      Pages: 52-55

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 根管壁象牙質切削片のDIAGNOdent^<TM> による測定-汚染度との関連性-2005

    • Author(s)
      木下潤一朗, 木村裕一, 永井香織, 那須裕弥, 山田嘉重, 渡辺治爾, 澤田英美, 中村幸生, 松本光吉
    • Journal Title

      日本歯科保存学会雑誌 48・3

      Pages: 362-371

    • NAID

      10016591458

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of diode laser irradiation on smear layer removal from root canal walls and apical leakage after obturation.2005

    • Author(s)
      Wang X, Sun Y, Kimura Y, Kinoshita J-I, Ishizaki NT, Matsumoto K
    • Journal Title

      Photomedicine and Laser Surgery 23・6

      Pages: 575-581

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A comparative study of the efficiency of FlexMaster NiTi rotary and K-Flexofile stainless steel hand instruments.2005

    • Author(s)
      Hossain MS, Matsumoto K, Kinoshita J-I, Kimura Y, Alam MS, Takamatsu Y
    • Journal Title

      Journal of Showa University Dental Society 25・4

      Pages: 267-274

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Morphological comparative study on ablation of dental hard tissues at cavity preparation by Er : YAG and Er, Cr : YSGG lasers.2005

    • Author(s)
      Harashima T, Kinoshita J-I, Kimura Y, Brugnera A, Zanin F, Pecora JD, Matsumoto K.
    • Journal Title

      Photomed Laser Surg 23

      Pages: 52-55

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Measurement of infected root canal dentin chips by DIAGNOdent.2005

    • Author(s)
      Kinoshita J-I, Kimura Y, Nagai K, Nasu Y, Yamada Y, Watanabe H, Sawada E, Nakamura Y, Matsumoto K.
    • Journal Title

      Japan J Conserv Dent 48

      Pages: 362-371

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of diode laser irradiation on smear layer removal from root canal walls and apical leakage after obturation.2005

    • Author(s)
      Wang X, Sun Y, Kimura Y, Kinoshita J-I, Ishizaki NT, Matsumoto K.
    • Journal Title

      Photomed Laser Surg 23

      Pages: 575-581

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A comparative study of the efficiency of FlexMaster NiTi rotary and K-Flexofile stainless steel hand instruments.2005

    • Author(s)
      Hossain MS, Matsumoto K, Kinoshita J-I, Kimura Y, Alam MS, Takamatsu Y.
    • Journal Title

      J Showa Univ Dent Soc 25

      Pages: 267-274

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Caries removal with Carisolv system : criteria evaluation and microleakage test.2005

    • Author(s)
      Yamada Y, Kimura Y, Hossain M, Kinoshita J-I, Shimizu Y, Matsumoto K.
    • Journal Title

      J Clin Pediat Dent 30

      Pages: 121-126

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of diode laser irradiation on smear layer removal from root canal walls and apical leakage after obturation.2005

    • Author(s)
      Wang X., Sun Y., Kimura Y., Kinoshita, JI., Ishizaki, NT., Matsumoto, K
    • Journal Title

      Photomedicine and Laser Surgery 23・6

      Pages: 575-581

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A comparative study of the efficiency of Flex Master NiTi rotary and K-Flexofile stainless steel hand instruments.2005

    • Author(s)
      Hossain, MS., Matsumoto, K., Kinoshita, JI., Kimura, Y., Alam MS., Takamatsu Y
    • Journal Title

      昭和歯学会雑誌 25・4

      Pages: 267-274

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 根管壁象牙質切削片のDIAGNOdent^<TM>による測定-汚染度との関連性-2005

    • Author(s)
      木下潤一朗, 木村裕一, 永井香織, 那須裕弥, 山田嘉重, 渡辺治爾, 澤田英美, 中村幸生, 松本光吉
    • Journal Title

      日本歯科保存学会雑誌 48・3

      Pages: 362-371

    • NAID

      10016591458

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Morphological comparative study on ablation of dental hard tissues at cavity preparation by Er : YAG and Er, Cr : YSGG lasers.2005

    • Author(s)
      Harashima T., Kinoshita J., Kimura Y., Brugnera A., Zanin F., Pecora J.D., Matsumoto K.
    • Journal Title

      Photomedicine and Laser Surgery 23・1

      Pages: 52-55

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Thermographical and morphological studies of Er,Cr : YSGG laser irradiation on root canal walls.2004

    • Author(s)
      Ishizaki NT, Matsumoto K, Kimura Y, Wang X, Kinoshita J, Okano SM, Jayawardena JA
    • Journal Title

      Photomedicine and Laser Surgery 22・4

      Pages: 291-297

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects on periradicular periodontal tissues of root canal irradiation with Er : YAG laser in rats.2004

    • Author(s)
      Kimura Y, Yonaga K, Murakoshi M, Yokoyama K, Watanabe H, Matsumoto K
    • Journal Title

      Photomedicine and Laser Surgery 22・4

      Pages: 335-341

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Thermographical and morphological studies of Er, Cr : YSGG laser irradiation on root canal walls.2004

    • Author(s)
      Ishizaki NT, Matsumoto K, Kimura Y, Wang X, Kinoshita J-I, Okano SM
    • Journal Title

      Photomed Laser Surg 22

      Pages: 291-297

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects on periradicular periodontal tissues of root canal irradiation with Er : YAG laser in rats.2004

    • Author(s)
      Kimura Y, Yonaga K, Murakoshi M, Yokoyama K, Watanabe H, Matsumoto K.
    • Journal Title

      Photomed Laser Surg 22

      Pages: 335-341

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects on periradicular periodontal tissues of root canal irradiation with Er : YAG laser in rats.2004

    • Author(s)
      Kimura Y., Yonaga K., Murakoshi M., Yokoyama K., Watanabe H., Matsumoto K.
    • Journal Title

      Photomedicine and Laser Surgery 22・4

      Pages: 335-341

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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