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Role of endothelial cells for nitric oxide production in periapical lesions

Research Project

Project/Area Number 16591923
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionNihon University

Principal Investigator

TAKEICHI Osamu  Nihon University, School of Dentistry, Assistant Professor, 歯学部, 講師 (10277460)

Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsvascular endothelial cadherin / inducible nitric oxide synthase / nitric oxide / periapical granulomas / HUVEC / NO inhibitors / immunohistochemistry / real time RCR / 誘導型一酸化窒素合成酵素 / 歯根のう胞 / E-セレクチン / 蛍光二重免疫染色 / 一酸化窒素合成酵素 / 根管滲出液 / RT-PCR法 / サイトスピン / ヘマトキシリン-エオジン染色
Research Abstract

The purpose of this study was to elucidate the role of endothelial cells for nitric oxide production in periapical lesions. Periapical exudates and periapical granulomas were analyzed, and inducible nitric oxide synthase (iNOS) and vascular endothelial (VE-) cadherin were examined in this study. Predominant cells isolated from periapical exudates were polymorphonuclear leukocytes (PMNs), as determined morphologically using hematoxylin-eosin stains. Protein expression and gene expression of iNOS was confirmed by immunocytochemical and RT-PCR analyses, respectively. Two-color immunohistochemistry using cryostat sections of periapical granulomas demonstrated that iNOS-expressing cells were macrophages, lymphocytes, fibroblasts, PMNs and endothelial cells whereas VE-cadherin-expressing cells were only endothelial cells. Thus, endothelial cells co-expressed iNOS and VE-cadherin. Real time PCR demonstrated the gene expression of iNOS and VE-cadherin mRNA in periapical granulomas. The expression levels of iNOS mRNA were higher than those of VE-cadherin. In our in-vitro study, human umbilical vein endothelial cells (HUVEC) were stimulated with interleukin-1 and Escherichia coli derived LPS for 2-24hr. Real time PCR demonstrated that iNOS and VE-cadherin mRNA was expressed in stimulated HUVEC. In addition, the expression levels of VE-cadherin mRNA were higher than that of iNOS. We also examined iNOS inhibitors in in-vitro study using HUVEC. The iNOS inhibitors decreased the expression levels of iNOS mRNA, therefore the possibility of pharmacological application was demonstrated. The data are consistent with a hypothesis suggesting that VE-cadherin expression of endothelial cells in periapical granulomas could be controlled in the association with nitric oxide.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (5 results)

All 2007 2006 2005

All Journal Article (5 results)

  • [Journal Article] Involvement of inducible nitric oxide synthase and receptor for advanced glycation end products in periapical granulomas2007

    • Author(s)
      Hama S, Takeichi O et al.
    • Journal Title

      Journal of Endodontics 33(2)

      Pages: 137-141

    • NAID

      10017439779

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Co-production of vascular endothelial cadherin and inducible nitric oxide synthase by endothelial cells in periapical granuloma2006

    • Author(s)
      Hama S, Takeichi O et al.
    • Journal Title

      International Endodontic Journal 39(3)

      Pages: 179-184

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mitogen-activated protein kinase/extracellular signal-regulated protein kinase (MAPK/ERK) activation of cultured human dental pulp cells by low-power Gallium-Aluminum-Arsenic (Ga-Al-As) laser irradiation2006

    • Author(s)
      Miyata H, Takeichi O et al.
    • Journal Title

      International Endodontic Journal 39(3)

      Pages: 238-244

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of mineral trioxide aggregate on proliferation of cultured human dental pulp cells2006

    • Author(s)
      Takita T, Takeichi O et al.
    • Journal Title

      International Endodontic Journal 39(5)

      Pages: 415-422

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Behaviour of bone marrow osteoblast-like cells on mineral trioxide aggregate : morphology and expression of type I collagen and bone-related protein mRNAs2005

    • Author(s)
      Nakayama A, Takeichi O et al.
    • Journal Title

      International Endodontic Journal 38(4)

      Pages: 203-210

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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