Characterization of Mallase's Epithelial Rest cell line and development to anti-tooth absorbing therapy.

• Project/Area Number: 16591926
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Kanagawa Dental College
• Principal Investigator: TSUNODA Akira Kanagawa Dental College, Dentistry, Lecturer, 歯学部, 講師 (70236933)
• Co-Investigator(Kenkyū-buntansha): SAITO Masahiro Osaka University, Graduate School dentistry, Graduate course, 大学院歯学研究科, 講師 (40215562) ; TERANAKA Toshio Kanagawa Dental College, Dentistry, Professor, 歯学部, 教授 (60104460) ; SUDA Naoto Tokyo Medical and Dental College, Dentistry, Lecturer, 大学院医歯学総合研究科, 講師 (90302885)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,400,000 (Direct Cost: ¥3,400,000); Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: ameloblast / epithelial rest / enamel / immortalization / root / development / dentin / tooth germ

Research Abstract

Mallase's Epithelial Rest cell (MMER) is an ameloblast that lined on a tooth root dentin. MMER found to express enamel protein such as amelogenin, however biological role of this cell is still unclear. Recently amelogenin knockout mice showed progressive tooth root absorption suggesting that enamel protein synthesized by MMER act to inhibit osteoclast activation on the tooth root surface. To investigate a biological role of MMER, we try to establish immortalized MMER cell line. MMER isolated from mice incisor root analog side, and immortalized with human papilloma virus E6 deleted with PDZ domain binding motif (MMER^<E6). MMER^<E6> able to grow more that population doubling 30, while MMER without immortalization stop to divide until population doubling 10. MMER expressed amelogenesis related gene such as enamelin, however no expression of amelogenenin was observed. These data indicated that MMER was successfully isolated, however culture condition that induced ameloblast differentiation should be required for investigating biological role of MMER.
On the other hand, we established immortalized mice dental epithelium cells (MDE) isolated form developing tooth germ at postnatal lday. MDE able to differentiate into amelobalst that expressed amelogenesis related genes such as amelogenin, ameloblastin and enamelin. These data indicating that MDE could serve as an experimental model for investigating function of MIMER.

Research Products

• [Journal Article] Establishment of Immortalized Dental Follicle Cells for Generating Periodontal Ligament In Vivo. (2007)
  • Author(s): T.Yokoi et al.
  • Journal Title: Cell and Tissue Res 327(2) Pages: 301-311
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Establishment of Immortalized Dental Follicle Cells for Generating Periodontal Ligament In Vivo. (2007)
  • Author(s): T.Yokoi, M.Saito, T.Kiyono, S.Iseki, K.Kosaka, E.Nishida, T.Tsubakimoto, H.Harada, K.Eto, T.Noguchi, T.Teranaka
  • Journal Title: Cell and Tissue Res. 327(2) Pages: 301-311
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of immortalized mouse dental follicle cells. (2005)
  • Author(s): T.Yokoi et al.
  • Journal Title: Bull. Kanagawa Den. Col. 33 Pages: 136-140
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular machines of Periodontal development, and its prospect of regenerative medicine for periodontium. (2005)
  • Author(s): M.Saito et al.
  • Journal Title: Bull. Kanagawa Dent. Col. 33 Pages: 31-35
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Formation of inhibition layers with a newly developed fluoride-releasing al-in-one adhesive. (2005)
  • Author(s): Mukai et al.
  • Journal Title: Dent Mater J 24 Pages: 172-177
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Immortalization of cementoblast progenitor cells with Bmi-1 and TERT. (2005)
  • Author(s): M.Saito
  • Journal Title: J Bone Miner Res 20 Pages: 50-57
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Differences between Alveolar and Iliac Bone Marrow Stromal Cells (2005)
  • Author(s): T.Matsubara
  • Journal Title: J Bone Miner Res 20 Pages: 399-409
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular mechanisms of Periodontal development, and its prospect of regenerative medicine for periodontium. (2005)
  • Author(s): M.Saito, A.Tsunoda, T.Yokoi, S.Hattori, T.Tsubakimoto, E.Nishida, K.Kousaka, M.Toyoda, S.Sato, T.Noguchi, T.Teranaka.
  • Journal Title: Bulletin of Kanagawa dental college. Vol.33(1) Pages: 31-35
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Immortalization of cementoblast progenitor cells with Bmi-1 and TERT. (2005)
  • Author(s): M.Saito, K.Handa, T.Kiyono, S.Hattori, T.Yokoi, T.Tsubakimoto, H.Harada, T.Noguchi, M.Toyoda, S.Sato, T.Teranaka
  • Journal Title: J Bone Miner Res 20(1) Pages: 50-57
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Alveolar Bone Marrow as a Cell Source for Regenerative Medicine : Differences between Alveolar and Iliac Bone Marrow Stromal Cells. J Bone Miner Res, 20(3), 399-409, 2005 (2005)
  • Author(s): T.Matsubara K.Suardita, M.Ishii, M.Sugiyama, A.Igarashi, R.Oda, M.Nishimura,.Saito M, K.Nakagawa, K.Yamanaka, K.Miyazaki, M.Shimizu, K.Tsuji, K.Nakamura, Y.Kato.
  • Journal Title: J Bone Miner Res 20(3) Pages: 50-57
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Formation of inhibition layers with a newly developed fluoride-releasing al-in-one adhesive. (2005)
  • Author(s): Mukai et al.
  • Journal Title: Dent Mater J. 24 Pages: 172-177
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of immortalized mouse dental follicle cells. (2005)
  • Author(s): T.Yokoi et al.
  • Journal Title: Bull. Kanagawa Dent. Col. 33 Pages: 136-140
• [Journal Article] Molecular mechanisms of Periodontal development, and its prospect of regenerative medicine for periodontium. (2005)
  • Author(s): M.Saito et al.
  • Journal Title: Bull. Kanagawa Dent. Col. 33 Pages: 31-35
• [Journal Article] Formation of inhibition layers with a newly developed fluoridc-releasing all-in-one adhesive. (2005)
  • Author(s): Mukai et al.
  • Journal Title: Dent Mater J 24 Pages: 172-177
• [Journal Article] Characterization of immortalized mouse dental follicle cells. (2005)
  • Author(s): T.Yokoi et al.
  • Journal Title: Bull.Kanagawa Dent.Col. 33 Pages: 136-140
• [Journal Article] Molecular mechanisms of Periodontal development, and its prospect of regenerative medicine for periodontium. (2005)
  • Author(s): M.Saito et al.
  • Journal Title: Bull.Kanagawa Dent.Col. 33 Pages: 31-35
• [Journal Article] Formation of inhibition layers with a newly developed fluoride-releasing all-in-one adhesive. (2005)
  • Author(s): Mukai et al.
  • Journal Title: Dent Mater J 24 Pages: 172-177
• [Journal Article] Immortalization of cementoblast progenitor cells with Bmi-1 and TERT. (2005)
  • Author(s): M.Saito et al.
  • Journal Title: J Bone Miner Res 20・1 Pages: 50-57
• [Journal Article] Alveolar Bone Marrow as a Cell Source for Regenerative Medicine : Differences between Alveolar and Iliac Bone Marrow Stromal Cells. (2005)
  • Author(s): T.Matsubara et al.
  • Journal Title: J Bone Miner Res 20・1 Pages: 399-409
• [Journal Article] Accelerated bone formation and increased osteoblast number contribute to the abnormalttooth germ development in parathyroid hormone-related protein (PTHrP)-knockout mice. (2004)
  • Author(s): Kitahara Y, Suda N, Terashima T, Mekaapiruk K, Hammond VE, Takano Y, Ohyama K
  • Journal Title: Bone 35・5 Pages: 1100-1106
• [Journal Article] Delayed tooth eruption and suppressed osteoclast number in the eruption pathway of eterozygous Runx2/Cbfa1 knockout mice. (2004)
  • Author(s): Yoda S, Suda N, Kitahara Y, Komori T, Ohyama K
  • Journal Title: Archs Oral Biol 49・6 Pages: 435-442
• [Journal Article] Molecular machines of periodontium development. -Analysis of cement genesis based on the cementum specific marker.-
  • Author(s): M.Saito et al.
  • Journal Title: Bull. Kanagawa Dent. Col. (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular machines of periodontium development. -Analysis of cement genesis based on the cementum specific marker.-
  • Author(s): M.Saito et al.
  • Journal Title: Bull. Kanagawa Dent. Col. (inpress)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular mechanisms of periodontium development.-Analysis of cementogenesis based on the cementum specific marker.
  • Author(s): M.Saito et al.
  • Journal Title: Bull. Kanagawa Dent. Col. (in press)
• [Journal Article] Molecular mechanisms of periodontium development. -Analysis of cementogenesis based on the cementum specific marker.
  • Author(s): M.Saito et al.
  • Journal Title: Bull.Kanagawa Dent.Col. (in press)