| Project/Area Number |
16591926
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
TSUNODA Akira Kanagawa Dental College, Dentistry, Lecturer, 歯学部, 講師 (70236933)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Masahiro Osaka University, Graduate School dentistry, Graduate course, 大学院歯学研究科, 講師 (40215562)
TERANAKA Toshio Kanagawa Dental College, Dentistry, Professor, 歯学部, 教授 (60104460)
SUDA Naoto Tokyo Medical and Dental College, Dentistry, Lecturer, 大学院医歯学総合研究科, 講師 (90302885)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | ameloblast / epithelial rest / enamel / immortalization / root / development / dentin / tooth germ |
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| Research Abstract |
Mallase's Epithelial Rest cell (MMER) is an ameloblast that lined on a tooth root dentin. MMER found to express enamel protein such as amelogenin, however biological role of this cell is still unclear. Recently amelogenin knockout mice showed progressive tooth root absorption suggesting that enamel protein synthesized by MMER act to inhibit osteoclast activation on the tooth root surface. To investigate a biological role of MMER, we try to establish immortalized MMER cell line. MMER isolated from mice incisor root analog side, and immortalized with human papilloma virus E6 deleted with PDZ domain binding motif (MMER^<E6). MMER^<E6> able to grow more that population doubling 30, while MMER without immortalization stop to divide until population doubling 10. MMER expressed amelogenesis related gene such as enamelin, however no expression of amelogenenin was observed. These data indicated that MMER was successfully isolated, however culture condition that induced ameloblast differentiation should be required for investigating biological role of MMER.
On the other hand, we established immortalized mice dental epithelium cells (MDE) isolated form developing tooth germ at postnatal lday. MDE able to differentiate into amelobalst that expressed amelogenesis related genes such as amelogenin, ameloblastin and enamelin. These data indicating that MDE could serve as an experimental model for investigating function of MIMER.
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