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Influence of Enzymes on the Biofilm Formation by the Isolated Bacteria from the Infected Root Canals.

Research Project

Project/Area Number 16591927
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionTsurumi University

Principal Investigator

HOSOYA Noriyasu  Tsurumi University, Senior lecture, 歯学部, 講師 (00157033)

Co-Investigator(Kenkyū-buntansha) IINO Fumiaki  Tsurumi University, Instructor, 歯学部, 助手 (20386915)
高橋 剛太  鶴見大学, 歯学部, 助手 (80288117)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsBiofilm / Enzyme / Bacteria of infected root canal / Electrophoresis / 根管分離細菌
Research Abstract

Objective : Biofilm formation in root canals is one of the main causes of intractable infected root canal cases. Biofilm is formed by Extracellular Polymeric Substances (EPS), which the bacteria produce and secrete outside cells. Structures and features of EPS differ depending on the bacteria forming them. In this study, we observed the influence of enzymes on EPS production by Porphyromonas gingivalis, and Staphylococcus aureus isolated from the infected
Materials and Methods : Inhibition effect ; A cell disk coated with collagen type I was suspended in nutrient medium containing pectinase (1%) or dextranase (1%), and cultured in an aerobic environment for 7 days. The surface of the cell disk was observed with a scanning electron microscope (SEM). Destructive effect; After culturing the cell disk in the medium for 7 days, pectinase and dextranase were added to the medium to reach a density of 1%. These were cultured for another 24 hours. The medium without enzyme was used as the control in both
Results and Conclusion : P.gingivalis, and S.aureus formed biofilms in all control groups. However, the formation of biofilm was different between bacteria. There was less EPS production by both bacteria cultured in medium containing 1% pectinase, compared with the control group. However, EPS production in medium containing 1% dextranase and in the control group was not different. The destructive effect of the enzyme for EPS was not evident. It is assumed that pectinase affects the process of EPS production by P.gingivalis and S.aureus.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (7 results)

All 2006 2005 2004

All Journal Article (7 results)

  • [Journal Article] 口腔内細菌由来のバイオフィルムに対する各種酵素の抑制効果2006

    • Author(s)
      飯野史明, 細矢哲康, 新井高
    • Journal Title

      日本歯科保存学会雑誌 49巻

      Pages: 200-200

    • NAID

      10020295010

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of Enzymes on the Biofilm Formation by the Isolated Bacteria from the Oral Cavity2006

    • Author(s)
      Fumiaki iino, Noriyasu Hosoya, Takashi Arai
    • Journal Title

      Japan Journal of Conservative Dentistry 49

      Pages: 200-200

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 口腔内細菌由来のバイオフィルムに対する各種酵素の抑制効果2006

    • Author(s)
      飯野史明, 細矢哲康, 新井 高
    • Journal Title

      日本歯科保存学雑誌 49巻・秋季特別号

      Pages: 200-200

    • NAID

      10020295010

    • Related Report
      2006 Annual Research Report
  • [Journal Article] アジスロマイシンによるPorphyromonas gingivalisのbiofilm形成抑制効果2005

    • Author(s)
      飯野史明 他
    • Journal Title

      日本歯周病学会会誌 47巻

      Pages: 97-97

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of Azithromycin on the control of biofilm formation by Porphyromonas gingivalis2005

    • Author(s)
      Fumiaki iino, Takashi Arai etc.
    • Journal Title

      Journal of The Japanese Society of Periodontology 47

      Pages: 97-97

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Relations Between Incidence of Black-Pigmented Anaerobic Rods and Symptoms on Traumatic Nonvital Teeth.2004

    • Author(s)
      Noriyasu Hosoya, Fumiaki iino, Nobuko Maeda, Takashi Arai
    • Journal Title

      Journal of Endodontics 30巻4号

      Pages: 283-283

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Relations Between Incidence of Black-Pigmented Anaerobic Rods and Symptoms on Traumatic Nonvital Teeth.2004

    • Author(s)
      Noriyasu Hosoya, Fumiaki iino, Nobuko Maeda, Takashi Arai
    • Journal Title

      Journal of Endodontics 30(4)

      Pages: 283-283

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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