| Project/Area Number |
16591929
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
NAKATA Kazuhiko Aichi Gakuin University, School of Dentistry, Assistant Professor, 歯学部, 講師 (70261013)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Dental Pulp / Dental Pulp Cell / Scaffold / Collagen Sponge / Cell-scaffold-complex / Tissue Engineering / Dental Pulp Tissue Reproduction Method / Endodontic Therapy / ティッシュ・エンジニアリング / 人造組織 |
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| Research Abstract |
The aim of present study was to develop "Cell-scaffold-complex" that used for the reproduction of the destructed dental pulp tissue. Human dental pulp (HDP) cells were obtained from freshly extracted human teeth removed for orthodontic reasons. Collagen sponge was prepared from pig' s skin collagen. "Cell-scaffold-complex" was prepared and its biocompatibility was analyzed.
The results were as follows :
1.Properties of human dental pulp (HDP) cells
The form of HDP cells was starlike or spindle type that was similar to human dermal fibroblasts. HDP cells had higher alkaline phosphatase (ALPase) activity compared with human dermal fibroblasts.
2.Properties of collagen sponge
The collagen sponge prepared in this study had small and irregular holes, which size was about 100 μm in peripheral zone and smaller in center part and had a close reticulation structure. The components of the collagen sponge were about 80% type-1collagen and 20% type-2 collagen.
3.HDP cells-scaffold-complex
The growth of HDP cells Into the collagen sponge was not observed. It was suggested that the improvement of the collagen sponge by using other materials, such as collagen gel, was necessary.
4.Biocompatibility of collagen sponge
The collagen sponge was significantly absorbed and the size was decreased. In histotropic study, advanced encapsulation by fibrous connective tissue was observed and the infiltration ambient inflammatory cells were not observed. Therefore, it was suggested that the biocompatibility of the collagen sponge was very excellent.
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