• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Dentine-pulp regeneration by differentiation of undifferentiated mesenchymal cells in a porous ceramic scaffold

Research Project

Project/Area Number 16591930
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionOsaka Dental University

Principal Investigator

TODA Tadao  Osaka Dental University, Faculty of dentistry, Professor, 歯学部, 教授 (70067023)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Masataka  Osaka Dental University, Faculty of dentistry, Lecturer, 歯学部, 講師 (70148451)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsCeramics / Bone marrow stem cells / Porous / Scaffold / Dentine / Dentine-Pulp complex / 多孔質セラミックス担体 / 未分化間葉細胞 / 再生 / 歯髄細胞
Research Abstract

Columnar porous ceramic scaffolds with and without a hollow center (8mm in diameter, 10mm in height, 55% total porosity, 150-300μm of pore size) were manufactured to use as scaffolds for regeneration of dentine-pulp complex. In vivo examinations, it was confirmed that configuration of a scaffold should influence for bone formation quantitatively in the pores.
Bone marrow cells were obtained from the bone shaft of rat femora. These scaffolds with the cells in the pores were implanted in dorsal subcutis of rats. The results showed that many pores including bone were recognized in the scaffolds with a hollow center but not in the scaffolds without a hollow center. Connective tissue infiltrated in a hollow center of the scaffolds and connective tissue including small vein was recognized in the pores. No connective tissue was recognized in the pores of scaffolds without a hollow center. On a surface of a wall contacting with surrounding tissue, a scaffold with a hollow center is more extensive than that without a hollow center. Connective tissue would infiltrate in the pores from the out side and the face of hollow center. It is thought that connective tissue delivers in the pores effectively the nutrition and the bioactive substance which are necessary for bone formation.
For regeneration of dentine-pulp complex and tooth root reconstruction, it is difficult to obtain many mesenchymal cells in dentistry. A long term may be necessary for the dentine formation in culture system. By the use of porous scaffolds with a hollow center, possibility to form hard tissue with a few mesenchymal cells was suggested in our studies. It considers this with extremely significant consequence in a future study toward dentine regeneration.
It is considered that the effects are extremely significant to realize regeneration of dentine-pulp complexes.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (17 results)

All 2006 2005 2004

All Journal Article (17 results)

  • [Journal Article] 気孔内における骨形成に及ぼす担体形状の影響2006

    • Author(s)
      松田孝之
    • Journal Title

      日本歯科保存学雑誌 49

      Pages: 126-139

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 多孔質担体気孔内骨形成にかかわるラミニンの影響-in vitro-2006

    • Author(s)
      好川正孝
    • Journal Title

      日本歯科保存学雑誌 49

      Pages: 140-148

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Configuration of a scaffold for bone formation in pores2006

    • Author(s)
      Matsuda Takayuki
    • Journal Title

      The Japanese Journal of Conservative Dentistry 49

      Pages: 126-139

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of laminin for osteogenesis in porous hydroxyapatite -in vitro-2006

    • Author(s)
      Yoshikawa Masataka
    • Journal Title

      The Japanese Journal of Conservative Dentistry 49

      Pages: 140-148

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 多孔質担体内骨形成にかかわるラミニンの影響2006

    • Author(s)
      好川正孝, 辻 則正, 松田孝之, 林 宏行, 戸田忠夫
    • Journal Title

      日本歯科保存学雑誌 49・1

      Pages: 140-148

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Morphology of porous hydroxyapatite scaffold for hard tissue formation2005

    • Author(s)
      Yoshikawa M
    • Journal Title

      Key Engineering Materials 284-286

      Pages: 321-324

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparison of hard tissue formation in two porous hydroxyapatite scaffolds treated with hyaluronic acid Sodium Salt2005

    • Author(s)
      Tsuji N
    • Journal Title

      Key Engineering Materials 284-286

      Pages: 961-964

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Porous hydroxyapatite scaffolds with laminin for osteogenesis2005

    • Author(s)
      M Yoshikawa
    • Journal Title

      Proceedings of the International Federation for Medical and Biological engineering 12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Nutrition supply for bone formation in porous hydroxyapatite scaffold2005

    • Author(s)
      T Toda
    • Journal Title

      Proceedings of the International Federation for Medical and Biological engineering 12

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparison of hard tissue formation in two porous hydroxyapatite scaffolds treated with hyaluronic acid sodium salt2005

    • Author(s)
      Tsuji N
    • Journal Title

      Key Engineering Materials 284-286

      Pages: 961-964

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Nutrition supply for bone formation in porous hydroxy -apatite scaffold2005

    • Author(s)
      T Toda
    • Journal Title

      Proceedings of the International Federation for Medical and Biological engineering 12

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Morphology of porous hydroxyapatite scaffold for hard tissue formation2005

    • Author(s)
      M.Yoshikawa, N.Tsuji, T.Toda, H.Machida, H.Ohgushi
    • Journal Title

      Key Engineering Materials 284-286

      Pages: 321-324

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Morphology of porous hydroxyapatite schaffold for hard tissue formation2005

    • Author(s)
      M.Yoshikawa, N.Tsuji, T.Toda, H.Machida, H.Ohgushi
    • Journal Title

      Key Engineering Materials 284-286

      Pages: 321-324

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Hard tissue formation by cultured dental Pulp Cells and bone marrow cells2004

    • Author(s)
      Yoshikawa M
    • Journal Title

      Journal of Qsaka Dental University 38

      Pages: 119-125

    • NAID

      110001717586

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Morphology of porous hydroxyapatite scaffold on the nutrition supply for hard tissue formation2004

    • Author(s)
      Masatska Yoshikawa
    • Journal Title

      Ceramics, Cells and Tissues 9

      Pages: 151-157

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Hard tissue formation by cultured dental Pulp cells and bone marrow cells2004

    • Author(s)
      Yoshikawa M
    • Journal Title

      Journal of Osaka Dental University 38

      Pages: 119-125

    • NAID

      110001717586

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Morphology of porous hydroxyapatite scaffold on the nutrition supply for hard tissue formation2004

    • Author(s)
      Masataka Yoshikawa
    • Journal Title

      Ceramics, Cells and Tissues 9

      Pages: 151-157

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi