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Research for the role of stress protein in TMD

Research Project

Project/Area Number 16591992
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionOsaka University (2005)
Kyoto University (2004)

Principal Investigator

YAMAMURA Isao  Osaka university, Faculty of dentistry, Assistant Professor, 大学院歯学研究科, 助手 (90332733)

Co-Investigator(Kenkyū-buntansha) NAGATA Kazuhiro  Kyoto University, Institute for Frontier medical Science, Professor, 再生医学研究所, 教授 (50127114)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsTMD / stress protein
Research Abstract

I confirmed HSP27, HSP70 and expression of HSP90 by RT-PCR method in the cultured synovial cells derived from the patients of temporomandibular arthrosis or TMJ synovialosteochondromatosis. And these results might be considered that these cells expressed the HSPs to be coped with stress of the outside. And I confirmed, Sex hormones, estrogen receptors ER-α and ER-β were expressed in these cells by RT-PCR method. HSP27 is suggesuted that it participates in the estrogen signal transduction. I confirmed induction of the expression of HSP27 gene by RT-PCR method in dose-dependent manner of β-estradiol when I examined influence to give to expression of HSP27 by estrogen, but the difference that was clear by Northen-Blot method was not recognized. To
Investigate this difference, I construct the plasmids consist of 5' promoter region of human HSP27 gene fused to a firefly luciferase gene, as a reporter gene, and analyzed the activatin of promoter activity of HSP27 gene by β-estradiol stimulation in the human synonvial cells. As the results, the increase of transcriptional activity by β-estradiol was confirmed in some cell lines, but was not in all cell lines. As this cause, the possibility that the mechanism of the transcriptional activation of HSP27 gene by β-estradiol having falls off it is originally thought about in each cell strain and is going to investigate a cause of the difference in future.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (35 results)

All 2006 2005 2004

All Journal Article (34 results) Book (1 results)

  • [Journal Article] Derlin-2 and Derlin-3 are regulated by the mammalian unfolded protein response and are required for ER-associated degradation.2006

    • Author(s)
      Oda Y
    • Journal Title

      J Cell Biol 172・3

      Pages: 383-393

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The maintenance of the endoplasmic reticulum network is regulated by p47, a cofactor of p97, through phosphorylation by cdc2 kinase.2005

    • Author(s)
      F.KANO et al.
    • Journal Title

      Genes Cells. 10(4)

      Pages: 333-44

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] NSF/SNAPs and p97/p47/VCIP135 are sequentially required for cell cycle-dependent reformation of the ER network.2005

    • Author(s)
      F.KANO
    • Journal Title

      Genes Cells. 10(10)

      Pages: 989-999

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gene expression in human keloids is altered from dermal to chondrocytic and osteogenetic lineage2005

    • Author(s)
      M.NAITOH et al.
    • Journal Title

      Genes Cells. 10(11)

      Pages: 1081-1091

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Specific recognition of the collagen triple helix by chaperone HSP47. Minimal structural requirement and spatial molecular orientation.2005

    • Author(s)
      T, KOIDE et al.
    • Journal Title

      J. Biol. Chem. 281(6)

      Pages: 3432-3438

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Retropharyngeal lymph node metastasis from oral cancer : A report of two patients2005

    • Author(s)
      M.Nishida et al.
    • Journal Title

      J Oral Maxillofac Surg. 63(3)

      Pages: 410-412

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The maintenance of the endoplasmic reticulum network is regulated by p47, a cofactor of p97, through phosphorylation by cdc2 kinase.2005

    • Author(s)
      KANO, H.KONDO, A.YAMAMOTO, AR.TANAKA, N.HOSOKAWA, K.NAGATA., M.MURATA.
    • Journal Title

      Genes Cells 10(4)

      Pages: 333-44

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] NSF/SNAPs and p97/p47/VCIP135 are sequentially required for cell cycle-dependent reformation of the ER network.2005

    • Author(s)
      KANO, H.KONDO, A.YAMAMOTO, Y.KANEKO, K.UCHIYAMA, N.HOSOKAWA, K.NAGATA, M.MURATA.
    • Journal Title

      Genes Cells 10(10)

      Pages: 989-999

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ene expression in human keloids is altered from dermal to chondrocytic and osteogenic lineage.2005

    • Author(s)
      NAITOH, H.KUBOTA, M.IKEDA, T.TANAKA, H.SIRANE, S.SUZUKI., K.NAGATA.
    • Journal Title

      Genes Cells 10(10)

      Pages: 1081-1091

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] pecific recognition of the collagen triple helix by chaperone HSP47.Minimal structural requirement and spatial molecular orientation.2005

    • Author(s)
      T, KOIDE, S.ASADA, Y.TAKAHARA, Y.NISHIKAWA, K.NAGATA., K.KITAGAWA.
    • Journal Title

      J.Biol.Chem 281(6)

      Pages: 3432-3438

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 2005, Retropharyngeal lymph node metastasis from oral cancer : A report of two patients2005

    • Author(s)
      M.Nishida, S.Yasuda, K.Murakami, I.Yamamura, Y.Nagata, T.Iizuka
    • Journal Title

      J Oral Maxillofac Surg 63(3)

      Pages: 410-412

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Retropharyngeal lymph node metastasis from oral cancer: A report of two patients2005

    • Author(s)
      M.Nishida
    • Journal Title

      J Oral Maxillofac Surg 63・3

      Pages: 410-412

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The maintenance of the endoplasmic reticulum network is regulated by p47, a cofactor of p97, through phosphorylation by cdc2 kinase.2005

    • Author(s)
      F.KANO
    • Journal Title

      Genes Cells 10・4

      Pages: 333-344

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Gene expression in human keloids is altered from dermal to chondrocytic and osteogenic lineage.2005

    • Author(s)
      M.NAITOH
    • Journal Title

      Genes Cells 10・11

      Pages: 1081-1091

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Proinsulin lacking the A7-B7 disulfide bond, Ins2 Akita, tends to aggregate due to the exposed hydrophobic surface.2005

    • Author(s)
      T.YOSHINAGA
    • Journal Title

      Biol. Chem 386・11

      Pages: 1077-1085

    • Related Report
      2005 Annual Research Report
  • [Journal Article] NSF/SNAPs and p97/p47/VCIP135 are sequentially required for cell cycle-dependent reformation of the ER network.2005

    • Author(s)
      F.KANO
    • Journal Title

      Genes Cells 10・10

      Pages: 989-999

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Retropharyngeal Lymph Node Metastases From oral Cancer : A report of 2 Patients2005

    • Author(s)
      Isao Yamamura et al.
    • Journal Title

      J Oral Maxillofac Surg 63

      Pages: 410-412

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Advanced glyceration end products increase collagen-specific chaperone protein in mouse diabetic nephropathy.2004

    • Author(s)
      S.Ohashi et al.
    • Journal Title

      J. Biol. Chem. 279(19)

      Pages: 19816-19823

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] CHOP induce death by prompting protein synthesis and oxidation in thestressed endoplasmic reticulum2004

    • Author(s)
      S.J.MARCINIAK et al.
    • Journal Title

      Genes Develop. 18(24)

      Pages: 3066-77

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Insufficient folding of type IV collagen nad formation of abnormal basement membrane-like structure in embryoid bodies derived from HSP47-nell ES cells.2004

    • Author(s)
      Y.MATSUOKA et al.
    • Journal Title

      Mol. Biol. Cell. 15

      Pages: 4467-4475

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of multiple members of the HSP70 family in playfishculture cells : molecular evolution of stress protein HPS70 in vertebrates.2004

    • Author(s)
      M.YAMASHITA et al.
    • Journal Title

      Gene 336

      Pages: 207-218

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Accumulation of type IV collagen in diluted endoplasmic reticulum leads to apoptosis HSP47-knockout mouse embryos through the induction of CHOP.2004

    • Author(s)
      T.MARUTANI et al.
    • Journal Title

      J. Cell. Sci. 117

      Pages: 5913-5922

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The endoplasmic reticulum stress response is stimulated through the continuous activation of transcription factors ATF6 and XBP1 in Ins2+/Akita pancreatic beta cells.2004

    • Author(s)
      J.NOZAKI et al.
    • Journal Title

      Genes Cells 9

      Pages: 261-270

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Advanced glycation end products increase collagen-specific chaperone protein in mouse diabetic nephropathy.2004

    • Author(s)
      OHASHI, H.ABE, T.TAKAHASHI, Y.YAMAMOTO, M.TAKEUCHI, H.ARAI, K.NAGATA, T.KITA, H.OKAMOTO, H.YAMAMOTO, T.DOI.
    • Journal Title

      J.Biol.Chem. 279(19)

      Pages: 19816-19823

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] CHOP induces death by promoting protein synthesis and oxidation in thestressed endoplasmic reticulum2004

    • Author(s)
      S.J.MARCINIAK, C.Y.YUN, S.OYADOMARI, I.NOVOA, Y.ZHANG, R.JUNGREIS, K.NAGATA, H.P.HARDING., D.RON.
    • Journal Title

      Genes Develop. 18(24)

      Pages: 3066-77

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Insufficient folding of type IV collagen nad formation of abnormal basement membrane-like structure in embryoid bodies derived from HSP47-null ES cells.2004

    • Author(s)
      MATSUOKA, H.KUBOTA, E.ADACHI, N.NAGAI, T.MARUTANI, N.HOSOKAWA., K.NAGATA.
    • Journal Title

      Mol.Biol.Cell. 15

      Pages: 4467-4475

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] haracterization of multiple members of the HSP70 family in platyfishculture cells : molecular evolution of stress protein HSP70 in vertebrates.2004

    • Author(s)
      YAMASHITA, K.HIRAYOSHI., K.NAGATA.
    • Journal Title

      Gene 336

      Pages: 207-218

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ccumulation of type IV collagen in dilated endoplasmic reticulum leads to apoptosis HSP47-knockout mouse embryos through the induction of CHOP.2004

    • Author(s)
      MARUTANI, A.YAMAMOTO, N.NAGAI, H.KUBOTA., K.NAGATA.
    • Journal Title

      J.Cell.Sci. 117

      Pages: 5913-5922

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The endoplasmic reticulum stress response is stimulated through the continuous activation of transcription factors ATF6 and XBP1 in Ins2+/Akita pancreatic beta cells.2004

    • Author(s)
      NOZAKI, H.KUBOTA, H.YOSHIDA, M.NAITOH, J.GOJO, T.YOSHINAGA, K.MORI, A.KOIZUMI., K.NAGATA.
    • Journal Title

      Genes Cells 9

      Pages: 261-270

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] CHOP induces death by promoting protein synthesis and oxidation in the stressed endoplasmic reticulum2004

    • Author(s)
      K.NAGAT et al.
    • Journal Title

      Genes Develop 15・18

      Pages: 3066-3077

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Accumulation of type IV collagen in dilated endoplasmic reticulum leads to apoptosis HSP47-Knockout mouse embryos2004

    • Author(s)
      K.NAGATA et al.
    • Journal Title

      J.Cell.Sci. 117

      Pages: 5913-5922

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of multiple members of the HSP70-family in platytish culture cells2004

    • Author(s)
      K.NAGATA et al.
    • Journal Title

      Gene 336

      Pages: 207-218

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Advanced glycation end products increase collagen-specific chaperone protein in mouse diabetic nephropathy2004

    • Author(s)
      K.NAGATA et al.
    • Journal Title

      J.Biol.Chem 279・19

      Pages: 19816-19823

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The ER stress response in stimulated through the continuous activation of transcription factors ATF6 and XBP1 in Ins2+/Akita pancreatic beta cells2004

    • Author(s)
      K.NAGATA et al.
    • Journal Title

      Genes Cells 9

      Pages: 261-270

    • Related Report
      2004 Annual Research Report
  • [Book] カラーアトラス 歯科と内科の接点を求めて2004

    • Author(s)
      山村 功ら
    • Total Pages
      254
    • Publisher
      永末書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

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