Molecular analysis of Wnt, Shh-Ptch signaling in Ameloblastoma

• Project/Area Number: 16591993
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: KYOTO UNIVERSITY
• Principal Investigator: NISHIDA Mitsuo KYOTO UNIVERSITY, Graduate School of Medicine, Associate Professor, 医学研究科, 講師 (50154620)
• Co-Investigator(Kenkyū-buntansha): SHIMIZU Akira KYOTO UNIVERSITY, Graduate School of Medicine, Professor, 医学研究科, 教授 (00162694) ; KOSUGI Shinzi KYOTO UNIVERSITY, Graduate School of Medicine, Professor, 医学研究科, 教授 (50252432) ; TAKAHASHI Katsu KYOTO UNIVERSITY, Graduate School of Medicine, Assistant Professor, 医学研究科, 助手 (90314202) ; SUGAI Manabu KYOTO UNIVERSITY, Graduate School of Medicine, Assistant Professor, 医学研究科, 助手 (90303891)
• Project Period (FY): 2004 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000); Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
• Keywords: ameloblastoma / CTNNB1 / PTCH1 / polymorphism / PTCH / β-カテニン / 体細胞突然変異

Research Abstract

Ameloblastoma is the most common odontogenic tumor, but the genetic nature of the changes in the tumor cells has been unclear. Mutations of the CTNNB1 or PTCH1 are observed in many human tumors. Both CTNNB1 and PTCH1 are important in tooth development and are expressed in ameloblastoma. The aim of this study was to investigate whether genetic alterations of CTNNB1 and PTCH1 are present in ameloblastoma. We investigated 14 cases of ameloblastoma. The polymorphisms found in the ameloblastoma patients were further examined in a subsequent case-control study. We found CTNNB1 mutation in one case of plexiform type ameloblastoma. CGG triplet repeat number polymorphism (CGG7/CGG8) in the 5'-untranslated region of PTCH1 was observed. The proportion of CGG8 alleles was significantly higher in the ameloblastoma group. The results of this study indicate a possible relationship between the CGG8 allele in PTCH1 and the risk for ameloblastoma.

Research Products

• [Journal Article] Uterine sensitization-associated gene-1 (USAG-1), a novel BMP antagonist expressed in the kidney, accelerates tubular injury (2006)
  • Author(s): Yanagita M., Okuda, T., Endo, S., Tanaka, M., Takahashi, K., Sugiyama, F., Kunita, S., Takahashi, S., Fukatsu, A., Yanagisawa M., Kita, T., Sakurai T
  • Journal Title: J Clin Invset. 116 Pages: 70-79
• [Journal Article] Germline mutations of the PTCH gene in Japanese atients with nevoid basal cell carcinoma syndrome (2005)
  • Author(s): Tanioka, M, Takahashi, K, Kosugi, S, Kawabata, T, Nishigori C, Miyachi, Y, Iizuka,T.
  • Journal Title: Arch.Dermatol,Res 296 Pages: 303-308
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Polymorphisms in PTCH1 affect the risk of Ameloblastoma (2005)
  • Author(s): Kawabata, T, Takahashi, K, Sugai, M, Murashima-Suginami, A, Ando, S, Shumizu, A, Kosugi, S, Sato, T, Nishida, M, Murakami, K, Iizuka,T.
  • Journal Title: J.Dent.Res 84 Pages: 812-816
  • NAID: 10019347219
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Retropharyngeal lymph node metastases from oral cancer : a report of 2 patients. (2005)
  • Author(s): Nishida M, Yasuda S, oMurakami K, Yamamura I, Nagata Y, Iizuka T
  • Journal Title: J Oral Maxillofac Surg. 63 Pages: 410-412
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Retropharyngeal lymph node metastases from oral cancer : a report of 2 patients. (2005)
  • Author(s): Nishida M, Yasuda S, Murakami K, Yamamura I, Nagata Y, Iizuka T.
  • Journal Title: J Oral Maxillofac Surg. 63(3) Pages: 410-412
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Polymorphisms in PTCH1 affect the risk of Ameloblastoma. (2005)
  • Author(s): Kawabata, T., Takahashi, K., Sugai, M., Murashima-Suginami, A., Ando, S., Shumizu, A., Kosugi, S., Sato, T., Nishida, M., Murakami, K., Iizuka, T.
  • Journal Title: J.Dent.Res. 84 Pages: 812-816
  • NAID: 10019347219
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Germline mutations of the PTCH gene in Japanese patients with nevoid basal cell carcinoma syndrome (2005)
  • Author(s): Tanioka, M., Takahashi, K., Kosugi, S., Kawabata, T., Nishigori C., Miyachi, Y., Iizuka, T.
  • Journal Title: Arch.Dermatol.Res 296 Pages: 303-308
• [Journal Article] Polymorphisms in PTCH1 affect the risk of Ameloblastoma. (2005)
  • Author(s): Kawabata, T., Takahashi, K., Sugai, M., Murashima-Suginami A., Ando, S., Shumizu., Kosugi, S., Sato, T., Nishida, M., Murakami, K., Iizuka.T.
  • Journal Title: J.Dent.Res 84 Pages: 812-816
  • NAID: 10019347219
• [Journal Article] Germline mutitians of the PTCH jene in Japanese petituts with neveid Desel cell carcinoma Syndrase (2005)
  • Author(s): Tanioka M.Takahashi K etc.
  • Journal Title: Arch Dermatol Kes 296・7 Pages: 303-308
• [Journal Article] Germline mutations of the PTCH gene in Japanese patients with nevoid basal cell carcinoma syndrome
  • Author(s): Tanioka, M., Takahashi, K., Kosugi, S., Kawabata, T., Nishigori C., Miyachi, Y., Iizuka, T.
  • Journal Title: Arch.Dermatol.Res. 296 Pages: 303-308
  • Description: 「研究成果報告書概要(欧文)」より