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Guided tissue regeneration with immobilized cytokine

Research Project

Project/Area Number 16591996
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionOkayama University

Principal Investigator

YAMACHIKA Eiki  Okayama University, Graduate school of Medicine, Dentistry and Pharmaceutical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 助手 (10294422)

Co-Investigator(Kenkyū-buntansha) TSUJIGIWA Hidetsugu  Okayama University, Graduate school of Medicine, Dentistry and Pharmaceutical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 助手 (70335628)
FUKUNAGA Joji  Okayama University, Graduate school of Medicine, Dentistry and Pharmaceutical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 助手 (10284069)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsBMP / Cytokine / Immobilization / bone / Regeneration / BMP-2 / I型アテロコラーゲン / 細胞活性 / 細胞内シグナル伝達 / Smad
Research Abstract

Bone morphogenetic protein 2(BMP-2) plays an important role in bone growth and regeneration. The creation of purified recombinant human BMP-2 (rhBMP-2) has led to advances in its potential for clinical use, however this protein is soluble and disperses soon after implantation. So rhBMP-2 can't have long time stimulation to BMP signaling cascade. To overcome this problem we reported the effects of immobilization of rhBMP-2 to succinylated type I atelocollagen
The mechanism of immobilized rhBMP-2 is based on the theories of covalent bonding between rhBMP-2 and succinylated type I atelocollagen. The immobilized rhBMP-2 binds with the receptor on the cell surface without being internalized into the cell, so it is able to transduce signals into the cell over a long period of time, achieving high efficiency in promoting cell proliferation.
We investigated the effect of immobilized rhBMP-2 by the way of (a) histological findings, (b) alkaline phosphatase (ALP) activity, (c) BMP intracellular signaling cascade (Smad1, Smad5 and phospho Smad1/5/8) expression, and (d) type I collagen, osteonectin, osteopontin, ostocalcin, and Smad1/5/8 mRNA expression.
Our results showed that (1) nonimmobilized and immobilized rhBMP-2 also increased the alkaline phosphatase (ALP) activity in ST2, MC3T3 and C2C12 cells. However significantly higher ALP activity was observed by the immobilized rhBMP-2 treatment. (2) Phosphorylation of Smad1/5/8 after nonimmobilized rhBMP-2 stimulation increased from 15 min after stimulation and decrease to the control revel after 6hrs, however the phosphorylation was prolonged by immobilized rhBMP-2 and its effect was detectable even after 48hrs.
So we conclude that because immobilized rhBMP-2 can emphasize and prolonged the effect of rhBMP-2, immobilizing rhBMP-2 is an efficient method to increase bone induction.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (20 results)

All 2006 2005 2004

All Journal Article (20 results)

  • [Journal Article] Immobilized rhBMP-2/succinylated type I atelocollagen gene expression of intracellular signaling molecules on ST2 cells.2006

    • Author(s)
      Tsujigiwa H
    • Journal Title

      J Biomed Master Res A. 77(3)

      Pages: 505-511

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immobilized rhBMP-2/succinylated type I atelocollagen gene expression of intracellular signaling molecules on ST2 cells.2006

    • Author(s)
      Tsujigiwa H
    • Journal Title

      J Biomed Mater Res A. 77 (3)

      Pages: 505-511

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells.2005

    • Author(s)
      Tsujigiwa H
    • Journal Title

      J Biomed Master Res A. 75(1)

      Pages: 210-215

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Frequent deletion and down-regulation of ING4, a candidate tumor suppressor gene at 12p13, in head and neck squamous cell carcinomas.2005

    • Author(s)
      Gunduz M
    • Journal Title

      Gene. 15;356

      Pages: 109-117

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regeneration of rat auditory ossicles using recombinant human BMP-2/collagen composites.2005

    • Author(s)
      Takeda Y
    • Journal Title

      J Biomed Master Res A. 73(2)

      Pages: 133-141

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells.2005

    • Author(s)
      Tsujigiwa H
    • Journal Title

      J Biomed Mater Res A. 75(1)

      Pages: 210-215

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Frequent deletion and down-regulation of ING4, a candidate tumor suppressor gene at 12p13, in head and neck squamous cell carcinomas.2005

    • Author(s)
      Gunduz M
    • Journal Title

      Gene. 15 ; 356

      Pages: 109-117

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regeneration of rat auditory ossicles using recombinant human BMP-2/collagen composites.2005

    • Author(s)
      Takeda Y
    • Journal Title

      J Biomed Mater Res A. 73(2)

      Pages: 133-141

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Frequent deletion and down-regulation of ING4, a candidate tumor suppressor gene at 12p13, in head and neck squamous cell carcinoma2005

    • Author(s)
      Yamachika E
    • Journal Title

      Gene 15 ; 356

      Pages: 109-117

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells2005

    • Author(s)
      Tsujigiwa H
    • Journal Title

      J Biomed Mater Res A. 75・(1)

      Pages: 210-215

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Osteogenic potential of periosteum graft in the rat calvarial model2005

    • Author(s)
      Fukunaga J
    • Journal Title

      Ann Plast Surg 54(1)

      Pages: 71-78

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Various immunostaining patterns of CD31, CD34 and endoglin and their relationship with lymph node metastasis in oral squamous cell carcinomas.2005

    • Author(s)
      Nagatsuka H
    • Journal Title

      J Oral Pathol Med. 34(2)

      Pages: 70-76

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Heparanase expression correlates with malignant potential in human colon cancer.2005

    • Author(s)
      Nobuhisa T
    • Journal Title

      J Cancer Res Clin Oncol. 131(4)

      Pages: 229-237

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Artemisinin : an alternative treatment for oral squamous cell carcinoma.2004

    • Author(s)
      Yamachika E
    • Journal Title

      Anticancer Res. 24(4)

      Pages: 2153-2160

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of osteoclast differentiation factor and osteoclastogenesis inhibitory factor in rat osteoporosis induced by immunosuppressant FK506.2004

    • Author(s)
      Fukunaga J
    • Journal Title

      Bone 34(3)

      Pages: 425-431

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of osteoclast differentiation factor and ostelclastogenesis inhibitory factor in rat osteoporosis induce by immunosuppressant FK506.2004

    • Author(s)
      Fukunaga J
    • Journal Title

      Bone. 34(3)

      Pages: 425-431

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Artemisinin : an alternative treatment for oral squamous cell carcinoma.2004

    • Author(s)
      Yamachika E
    • Journal Title

      Anticancer Research 24(4)

      Pages: 2153-2160

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of osteoclast differentiation factor and osteoclastogenesis inhibitory factor in rat osteoporosis induced by immunosuppressant FK506.2004

    • Author(s)
      Fukunaga J.
    • Journal Title

      Bone 34(3)

      Pages: 425-431

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genetic and epigenetic alterations of BRG1 promote oral cancer development.2004

    • Author(s)
      Gunduz E
    • Journal Title

      Int J Oncol. 26(1)

      Pages: 201-210

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vitro response of osteoblast-like and odontoblast-like cells to unsubstituted and substituted apatites.2004

    • Author(s)
      Inoue M
    • Journal Title

      J Biomed Mater Res. 70A(4)

      Pages: 585-593

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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