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The analysis of the graft from the viewpoint with the relation between malignancy of oral carcinoma and defensin

Research Project

Project/Area Number 16591998
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

MIZUKAWA Nobuyoshi  Okayama University, University hospital of Medicine and Dentistry, Assistant Professor, 医学部・歯学部附属病院, 講師 (00263608)

Co-Investigator(Kenkyū-buntansha) SUGAHARA Toshio  Okayama University, Graduate school of Medicine, Dentistry and Pharmaceutical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (10116048)
YAMAAI Yuichiro  Okayama University, Graduate school of Medicine, Dentistry and Pharmaceutical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 助手 (00158057)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsantimicrobial peptide / defensin / HBD / HNP / graft / oral carcinoma / 移植再生 / HBD-2
Research Abstract

To identify the location of human-beta-defensin-2 and human alpha-defensins in odontogenic keratocysts and radicular cysts. Human beta-defensin-2 immunoreactivity was evident in odontogenic keratocysts(72%), and in radicular cysts(37%). Signals for human beta-defensin-2 were detected in the cytoplasm of keratinocytes in the parakeratinised and granular layers in both types of cysts. The localization of human beta-defensin-2 did not differ between cysts and normal mucosa. Human alpha-defensin immunoreactivity was detected in both types of cysts, but not in keratinocytes, Signals for human aloha-defensins were detected in the cytoplasm of neutrophils in the lamina propria. Many cysts generate human beta-defensin-2 and human aloha-defensins suggesting that similar defense mechanisms utilizing defensins exist in both types of cysts, as well as in normal oral mucosa.
Next study was to demonstrate the immunohistochemical localization and distribution of human beta-defensins, peptides with antimicrobial activity, in Sjogren's syndrome and Xerostomia tissues. Tissue samples were embedded in paraffin and beta-defensins were immunostained by the streptavidin-biotin coupled peroxidase method. Labial glands in Xerostomia patients and control patients(non- Xerostomia and non- Sjogren's syndrome) were stained with anti-beta-defensins antibody. However, labial glands in Sjogren's syndrome were not stained with anti-beta-defensins antibody.
Therefore, beta-defensins have been suggested to be important factors in Sjogren's syndrome.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (15 results)

All 2005 2004

All Journal Article (15 results)

  • [Journal Article] Clinial effects of cevimeline hydrochloride for the treatment of dry mouth in 2 patients with Sjogren's syndrome.2005

    • Author(s)
      Mizukawa N
    • Journal Title

      J Hard tissue biology 14.2

      Pages: 58-59

    • NAID

      110005853492

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Two cases of jaw reconstruction using vascularized fibular bone graft and implant.2005

    • Author(s)
      Takagi S
    • Journal Title

      J Hard tissue biology 14.2

      Pages: 64-64

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Two cases of jaw reconstruction using vascularized fibular bone graft and implant.2005

    • Author(s)
      Takagi S
    • Journal Title

      J Hard tissue biology 14(2)

      Pages: 64-64

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Clinical Effects of Cevimeline Hydrochloride for the Treatment of Dry Mouth in 2 patients with Sjogren's Syndrome2005

    • Author(s)
      Nobuyoshi Mizukawa
    • Journal Title

      Journal of hard tissue biology 14(2)

      Pages: 134-135

    • Related Report
      2005 Annual Research Report
  • [Journal Article] β-TCPを用いてインプラント周囲欠損骨の骨再生を行った1症例2005

    • Author(s)
      植野高章
    • Journal Title

      岡山歯学会雑誌 24(2)

      Pages: 71-73

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Two Cases of Jaw Reconstruction Using Yascularized Fibular Bone Graft and Implant2005

    • Author(s)
      Shin Takagi
    • Journal Title

      Journal of hard tissue biology 14(2)

      Pages: 64-64

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of human α-and β-defensins in odontogenic keratocysts and radicular cysts.2004

    • Author(s)
      Yoshimoto T
    • Journal Title

      Asian J oral maxillofac Surg 16

      Pages: 242-247

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regulation of bone metabolism in immunosuppressant(FK506) -treated rats.2004

    • Author(s)
      Kirino S
    • Journal Title

      Journal of Bone and Mineral Metabolism 22

      Pages: 554-560

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immunolocalization of vascular endothelial growth factor during heterotopic bone formation induced from grafted periosteum.2004

    • Author(s)
      Ueno T
    • Journal Title

      Ann Plast Surg 53.2

      Pages: 150-154

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Pathological change of articular cartilage in the mandibular head treated with immunosuppressant FK506.2004

    • Author(s)
      Ueno T
    • Journal Title

      Histol and Histopathol 19.1

      Pages: 15-21

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immunolocalization of vascular endothelial growth factor during heterotopic bone formation induced from grafted periosteum.2004

    • Author(s)
      Ueno T
    • Journal Title

      Ann Plast Surg 53(2)

      Pages: 150-154

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Pathological change of articular cartilage in the mandibular head treated with immunosuppressant FK506.2004

    • Author(s)
      Ueno T
    • Journal Title

      Histol and Histopathol 19(1)

      Pages: 15-21

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Clinial effects of cevimeline hydrochloride for the treatment of dry mouth in 2 patients with Sjogren's syndrome.2004

    • Author(s)
      Mizukawa N
    • Journal Title

      J Hard tissue biology 14(2)

      Pages: 58-59

    • NAID

      110005853492

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of Human α- and β-defensins in Odontogenic Keratocysts and Radicular cysts2004

    • Author(s)
      Yoshimoto T, Mizukawa N, Sugahara T, Yamaai T.
    • Journal Title

      Asian J Oral Maxillofac Surg 16

      Pages: 241-246

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Immunolocation of vascular endothelial grouwth factor during heterotopic bone formation induced from grafted periosteum.2004

    • Author(s)
      Ueno T., Mizukawa N., Sygahara T
    • Journal Title

      Annals of Plastic Surgery. 53

      Pages: 150-154

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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