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ESTABLISHMENT OF ANTI-LYMPHANGIOGENETIC THERAPY BY CANCER SPECIFIC s-VEGFR-3.

Research Project

Project/Area Number 16592020
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionSHOWA UNIVERSITY

Principal Investigator

HATORI Masashi  Showa Univ., Dept Oral Maxillofac Surg., Assistant Professor, 歯学部, 講師 (20245814)

Co-Investigator(Kenkyū-buntansha) ITO Daisuke  Showa Univ., Dept Oral Maxillofac Surg., Assistant Professor, 歯学部, 講師 (40286844)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsVFGF / VEGFR-3 / LYMPHANGIOGENESIS / HGF / c-Met / HIF / SQUAMOUS CELL CARCINOMA / MMP / HIF-1α
Research Abstract

Expression of VEGF family in OSCC cell lines.
To examine the expression profile of VEGF family on squamous cell carcinoma cell lines NA and HSC-4, we performed RT-PCR analysis. As results, both cells expressed VEGF-C and VEGF-D. The expression of VEGFR was not detected in both cells.
Involvement of HGF-Met system in the invasiveness of OSCC cells.
It is well known that the expression of VEGF is highly regulated by c-Met, a counter part of hepatocyto growth factor (HGF). Thus, we examined the expressions of c-Met and HGF by RT-PCR in both OSCC cells. Although HGF was not expressed in OSCC cells, c-Met was highly expressed in both cells.
The cancer cells survive in the situation of low oxygen called HYPOXIA in tumor mass. The cells under hypoxia express HIF, a nuclear factor and acquire the survival potential. HIF exist in upstream of c-Met,MMPs,VEGF and influence the invasiveness of cancer cells. Therefore, we performed RT-PCR for HIF on OSCC cells in hypoxia chamber. Both cells expressed HIF-1 in hypoxia and c-Met is also induced. Interestingly, invasion assay in hypoxia chamber revealed highly invasiveness of OSCC cells. To examine the mechanism of this up-regulation of invasiveness, we performed RT-PCR, western blotting and ELISA for MMP-2, a major matrix metalloproteinase for resolution of basement membrane in human. As results, the cells cultured in hypoxia chamber expressed high concentration of MMP-2 in the culture supernatant.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (13 results)

All 2006 2005 2004 Other

All Journal Article (13 results)

  • [Journal Article] 選択的COX-2阻害はMMP-9の発現低下を介して口腔扁平上皮癌細胞の浸潤能を抑制する.2006

    • Author(s)
      齋藤雅子, 羽鳥仁志, 衣笠有里子, 栗原祐史, 伊藤秀寿, 南雲正男
    • Journal Title

      昭和歯学会雑誌 26

      Pages: 1-4

    • NAID

      10019605269

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 選択的COX-2阻害はMMP-9の発現低下を介して口腔扁平上皮癌細胞の浸潤能を抑制する2006

    • Author(s)
      齋藤雅子, 羽鳥仁志, 衣笠有里子, 栗原祐史, 伊藤秀寿, 南雲正男
    • Journal Title

      昭和歯学会雑誌 26・1

      Pages: 1-4

    • NAID

      10019605269

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cyclooxygenase-2 : A potential target in the treatment of oral cancers.2005

    • Author(s)
      Hatori M, Nagumo M
    • Journal Title

      Oral Sci Int 2

      Pages: 1-7

    • NAID

      130004503803

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 口腔扁平上皮癌細胞に対するCOX-2阻害療法の基礎的検討2005

    • Author(s)
      衣笠有里子, 羽鳥仁志, 伊藤秀寿, 栗原祐史, 南雲正男
    • Journal Title

      日本口腔組織培養学会誌 14

      Pages: 29-30

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Cyclooxygenase-2 : A potential target in the treatment of oral cancers.2005

    • Author(s)
      Hatori M, Nagumo M
    • Journal Title

      Oral Science International 2

      Pages: 1-7

    • NAID

      130004503803

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cyclooxygenase-2: A potential target in the treatment of oral cancers.2005

    • Author(s)
      Hatori M, Nagumo M
    • Journal Title

      Oral Sci Int 2

      Pages: 1-7

    • NAID

      130004503803

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Inhibition of cyclooxygenase-2 suppresses invasiveness of oral squamous cell carcinoma cell lines via down-regulation of MMP-2 and CD442005

    • Author(s)
      Kinugasa, Y.Hatori, M.et al.
    • Journal Title

      Clinical and Experimental Metastasis (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inhibition of cyclooxygenase-2 suppresses invasiveness of oral squamous cell carcinoma cell lines via down-regulaion of matrix metalloproteinase-2 and CD44.2004

    • Author(s)
      Kinugasa Y, Hatori M, Ito H, Kurihara Y, Ito D, Masao Nagumo
    • Journal Title

      Clin Exp Metastasis 21

      Pages: 737-745

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of 5-fluorouracil on G1 phase cell cycle regulation in oral cancer cell lines.2004

    • Author(s)
      Li M-H, Ito D, Sanada M, Odani T, Hatori M, Iwase M, Nagumo M
    • Journal Title

      Oral Oncol. 40

      Pages: 63-70

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibition of cyclooxygenase-2 suppresses invasiveness of oral squamous cell carcinoma cell lines via down-regulation of matrix metalloproteinase-2 and CD44.2004

    • Author(s)
      Kinugasa Y, Hatori M, Ito H, Kurihara Y, Ito D, Masao Nagumo
    • Journal Title

      Clinical and Experimental Metastasis 21

      Pages: 737-745

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of 5-fluorouracil on G1 phase cell cycle regulation in oral cancer cell lines.2004

    • Author(s)
      Li M-H, Ito D, Sanada M, Odani T, Hatori M, Iwase M, Nagumo M
    • Journal Title

      Oral Oncology 40

      Pages: 63-70

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Thalidomide suppresses melanoma growth by activating natural killer cells in mice.

    • Author(s)
      Kawamata A, Odani T, Isobe T, Iwase M, Hatori M, Nagumo M, Ito D
    • Journal Title

      J Immnol. In submission

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Thalidomide suppresses melanoma growth by activating natural killer cells in mice.

    • Author(s)
      Kawamata A, Odani T, Isobe T, Iwase M, Hatori M, Nagumo M, Ito D
    • Journal Title

      Journal of immunology (in submission)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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