| Project/Area Number |
16592031
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
MORITA Shosuke Osaka Dental University, Dentistry, Professor, 歯学部, 教授 (90148461)
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| Co-Investigator(Kenkyū-buntansha) |
TABATA Yasuhiko Kyoto University, Institute for Frontier Medical Sciences, Professor, 再生医科学研究所, 教授 (50211371)
MUSHIMOTO Kozo Osaka Dental University, Dentistry, Associate Professor, 歯学部, 助教授 (30067061)
HOKUGO Akishige Kyoto University, Institute for Frontier Medical Sciences, Researcher, 再生医科学研究所, 研究員 (10378903)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Stem cells / Biomaterials / Reconstruction / 血管柄付き移植骨 / 間葉系幹細胞 |
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| Research Abstract |
1. We designed prefabricated vascularized bone grafts by the combined use of collagen sponge scaffold, osteoblasts differentiated from rat bone marrow, and a vascular bundle in a diffusion chamber. In the experimental group, saphenous vessels of were exposed and placed on slits cut into the diffusion chamber. Then, a collagen sponge scaffold seeded with osteoblasts was packed into the diffusion chamber. In the control group, this was performed without the placement of blood vessels. The implants were harvested and new bone formation was assessed. In the experimental group, active bone formation in the diffusion chamber was observed. Microvessels had formed throughout the new bone tissue. In contrast, there was no significant bone formation in the control group. These results suggest that the combination of collagen scaffold, osteoblasts, and a vascular bundle in the chamber may be a useful method to prefabricate vascularized bone grafts.
2. We also created prefabricated vascularized bone grafts with β-tricalcium phosphate (βTCP) as a substitute for the collagen sponge. However, less bone formation was observed when compared to the collagen sponge. We conclude that the collagen sponge is a promising scaffold for the preparation of prefabricated vascularized bone grafts.
3. We also performed a transplantation experiment. When a prefabricated vascularized bone graft using a combination of β-TCP and PCBM was onlay-grafted to the femur of the same rat, the prefabricated vascularized bone graft directly fused to the cortical surface of the femur, with no intervening fibrous tissue. This finding suggests that a prefabricated vascularized bone graft design is a viable graft material for bone reconstruction.
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