| Project/Area Number |
16592039
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SUSAMI Takafumi The University of Tokyo, Faculty of Medicine, Assistant Professor, 医学部附属病院, 助教授 (80179184)
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| Co-Investigator(Kenkyū-buntansha) |
OGIHARA Yuji The University of Tokyo, Faculty of Medicine, Assistant, 医学部附属病院, 助手 (20345226)
TAKATO Tsuyoshi The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (90171454)
HOSHI Kazuto The University of Tokyo, Faculty of Medicine, visiting Assistant Professor, 医学部附属病院, 寄付講座教員 (30344451)
OGASAWARA Toru The University of Tokyo, Faculty of Medicine, Contribute lectures staff, 医学部附属病院, 寄付講座教員 (20359623)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Dentistry / Regenerative Medicine / Bone Metabolism / 再生医学 / 再生骨軟骨 / 液性因子 / 脱分化 / 口唇口蓋裂 / 統計的手法 / 細胞分化 |
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| Research Abstract |
The purposes of this study were ; (1) how to make tissue-engineered bone in order to apply regenerative medicine to bone graft for CLP patients and (2) to establish the system(s) to evaluate such application from an orthodontic viewpoint.
First, to prepare a mesenchymal cell proliferation medium that does not contain fetal bovine serum (FBS), and that provides more than a 1000-fold increase in cell numbers within approximately a month, we investigated the combinational effects of 12 factors on the proliferation of human cells by analysis of variance in fractional factorial design. As a result, FGF-2, dexamethasone, insulin, and IGF-I possessed promotional effects on proliferation on chondrocytes, while the combination of FGF-2 with insulin or IGF-I synergistically enhanced the proliferation. Thus we concluded that such a combination of FGF-2 with insulin or IGF-I may be useful for promotion of auricular chondrocyte proliferation in a clinical application for cartilage regeneration. Next we discovered that the combination of BMP-2, insulin, and thyroid hormone (BIT) is ideal to deal with the problem of de-differentiation of the cell caused along with the cell proliferation culture. Then, we clarified the characteristics of some hydro-gels expected to be used in clinical application by studying their effects on cell proliferation or differentiation. Moreover, we reported that CGK2 plays an important role on chondrocyte differentiation and that the combination of Cdk4 and cyclin D1 could be used in bone regeneration.
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