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Regeneration of bone and periodontium using thermodynamics and mechanical stress

Research Project

Project/Area Number 16592041
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

WARITA Hiroyuki  Tokyo Medical and Dental University, Graduate School, Orthodontic Science, Assistant Professor, 大学院・医歯学総合研究科, 助教授 (30262207)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Yoshiro  Tokyo Medical and Dental University, Dental Hospital, Orthodontics, Lecturer, 歯学部附属病院, 講師 (20292980)
KANNO Zuisei  Tokyo Medical and Dental University, Graduate School, Orthodontic Science, Assistant, 大学院・医歯学総合研究科, 助手 (40345301)
KANEKO Sawa  Tokyo Medical and Dental University, Graduate School, Orthodontic Science, Assistant, 大学院・医歯学総合研究科, 助手 (80372449)
HISANO Masataka  Tokyo Medical and Dental University, Graduate School, Orthodontic Science, Lecturer, 大学院・医歯学総合研究科, 講師 (80282763)
TAKESHI MURAMOTO  Tokyo Medical and Dental University, Graduate School, Orthodontic Science, Assistant, 大学院・医歯学総合研究科, 助手 (30345300)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordsmechanical stress / bone / periodontium / regeneration / tooth transplantation / occlusal stimuli / alveolar bone / 咬合機能低下 / 加齢 / 組織の回復再生 / 血液循環 / サーモダイナミクス
Research Abstract

In order to clarify the regenerative response of bone and periodontium against mechanical stress, molecular biological and histological examinations were performed. Mechanical tensional stress of low magnitude induced the increase of both type I collagen and decorin mRNA expression without changing ALP activity in periodontal ligament cells. Mechanical tensional stress of high magnitude induced the increase of type I collagen and decorin mRNA expression while decreasing ALP activity. mRNA level of Cbfa1/Runx2 increased at lower magnitudes of strain (0.8% and 5% elongation) but decreased at higher magnitude of strain (15% elongation). At 24 and 48 h, Cbfa1/Runx2 and osteocalcin mRNAs decreased at 5%, 10%, and 15% elongation, whereas cell proliferation and expression of type I collagen mRNA increased at the same elongation.
the replanted teeth were given a one-week healing period and then occlusion recovery was assessed, which resulted in decreased ankylosis and root resorption. The proliferative activity of PDL cells in the occluded group was generally higher than in the nonoccluded group. The activity of PDL cells in the recovery group was also higher than that of the nonoccluded group. the replanted teeth were given a one-week healing period and then occlusion recovery was assessed, which resulted in decreased ankylosis and root resorption. The proliferative activity of PDL cells in the occluded group was generally higher than in the nonoccluded group. The activity of PDL cells in the recovery group was also higher than that of the nonoccluded group.
In the hypofunctional condition of occlusal stimuli, marrow spaces had widened and elongated vertically : the activity of bone formation had decreased. On the other hand, in the recovery of occlusal function, the elongation of teeth was decreased and the activity of bone formation and regeneration had promoted.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (21 results)

All 2005 2004 Other

All Journal Article (21 results)

  • [Journal Article] Occlusal forces promote periodontal healing of transplanted teeth with enhanced nitric oxide synthesis.2005

    • Author(s)
      Chen CC, Kanno Z, Soma K
    • Journal Title

      Journal of Medical and Dental Sciences 52(1)

      Pages: 59-64

    • NAID

      110001065277

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Modulation of extracellular matrix synthesis and alkaline phosphatase activity of periodontal ligament cells by mechanical stress.2005

    • Author(s)
      Ozaki S, Kaneko S, Podyma-Inoue KA, Yanagishita M, Soma K
    • Journal Title

      Journal of Periodontal Research 40(2)

      Pages: 110-117

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Effects of mechanical strain on proliferation and differentiation of bone marrow stromal cell line ST2.2005

    • Author(s)
      Koike M, Shimokawa H, Kanno Z, Ohya K, Soma K
    • Journal Title

      Journal of Bone and Mineral Metabolism 23(3):

      Pages: 219-225

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Occlusal Forces Promote Periodontal Healing of Transplanted Teeth and Prevent Dentoalveolar Ankylosis : An Experimental Study in Rats.2005

    • Author(s)
      Mine K, Kanno Z, Muramoto T, Soma K
    • Journal Title

      Angle Orthodontist 75(4)

      Pages: 637-644

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Influence of Occlusal Stimuli on the Remodelling of Alveolar Bone in a Rat Hypofunction-Recovery Model. Journal of Oral Biosciences2005

    • Author(s)
      Enokida M, Kaneko S, Yanagishita M, Soma K
    • Journal Title

      Journal of Oral Biosciences 47(4)

      Pages: 321-334

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 咬合刺激が成長期歯槽骨および顎骨の骨形成能に及ぼす影響について2005

    • Author(s)
      下元康英, 岩崎祐子, 村本 健, 鄭朱玲, 相馬邦道
    • Journal Title

      日本骨形態計測学会雑誌 15(1)

      Pages: 29-34

    • NAID

      10018374769

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Effects of Mechanical Strain on Proliferation and Differentiation of Bone Marrow Stromal Cell Line ST2.2005

    • Author(s)
      Koike M, Shimokawa H, Kanno Z, Ohya K, Soma K.
    • Journal Title

      Journal of Bone and Mineral Metabolism 23(3)

      Pages: 219-225

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Modulation of extracellular matrix synthesis and alkaline phosphatase activity of periodontal ligament cells by mechanical stress.2005

    • Author(s)
      Ozaki S, Kaneko S, Podyma-Inoue K A, Yanagishita, Soma K.
    • Journal Title

      Journal of Periodontal Research 40(2)

      Pages: 110-117

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Occlusal Forces Promote Periodontal Healing of Transplanted Teeth and Prevent Dentoalveolar Ankylosis : An Experimental Study in Rats.2005

    • Author(s)
      Mine K, Kanno Z, Muramoto T, Soma K.
    • Journal Title

      Angle Orthod 75(4)

      Pages: 545-552

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Occlusal Forces Promote Periodontal Healing of Transplanted Teeth with Enhanced Nitric Oxide Synthesis.2005

    • Author(s)
      Chen CC, Kanno Z, Soma K.
    • Journal Title

      J Med Dent Sci 52(1)

      Pages: 59-64

    • NAID

      110001065277

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Influence of Occlusal Stimuli on the Remodeling of Alveolar Bone in a Rat Hypofunction-Recovery Model.2005

    • Author(s)
      Enokida M, Kaneko S, Yanagishita M, Soma K
    • Journal Title

      Journal of Oral Biosciences 47(4)

      Pages: 321-334

    • NAID

      10016884868

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of mechanical strain on proliferation and differentiation of bone marrow stromal cell line ST2.2005

    • Author(s)
      Koike M, Shimokawa H, Kanno Z, Ohya K, Soma K
    • Journal Title

      Journal of Bone and Mineral Metabolism 23(3)

      Pages: 219-225

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Influence of Occlusal Stimuli on the Remodelling of Alveolar Bone in a Rat Hypofunction-Recovery Model.2005

    • Author(s)
      Enokida M, Kaneko S, Yanagishita M, Soma K
    • Journal Title

      Journal of Oral Biosciences 47(4)

      Pages: 321-334

    • NAID

      10016884868

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Occlusal Forces Promote Periodontal Healing of Transplanted Teeth with Enhanced Nitric Oxide Synthesis.2005

    • Author(s)
      Chen C.C.
    • Journal Title

      Journal of Medical and Dental Science 52・1

      Pages: 59-64

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Occlusal Forces Promote Periodontal Healing of Transplanted Teeth and Prevent Dentoalveolar Ankylosis : an Experimental Study in Rats.2005

    • Author(s)
      Mine K.
    • Journal Title

      Angle Orthodontist 75・4

      Pages: 545-552

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of Mechanical Strain on Proliferation and Differentiation of Bone Marrow Stromal Cell Line ST2.2005

    • Author(s)
      Koike M.
    • Journal Title

      Journal of Bone and Mineral Metabolism (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Modulation of extracellular matrix synthesis and alkaline phosphatase activity of periodontal ligament cells by mechanical stress.2005

    • Author(s)
      Ozaki S
    • Journal Title

      Journal of Periodontal Research (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Nitric Oxide Synthase Expression is Increased by Occlusal Force in Rat Periodontal Ligament.2004

    • Author(s)
      Warita H, Watarai H, Soma K.
    • Journal Title

      Orthod Craniofac Res 7

      Pages: 122-126

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effect of Nitric Oxide on the Recovery of the Hypofunctional Periodontal Ligament.2004

    • Author(s)
      Watarai H, Warita H, Soma K.
    • Journal Title

      J Dent Res 83(4)

      Pages: 338-342

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Age-related changes and the possible adaptability of rat jaw muscle spindles : immunohistochemical and fine structural studies.2004

    • Author(s)
      Winarakwong L.
    • Journal Title

      Archives of Histology and Cytology 67・3

      Pages: 227-240

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Occlusal stimuli affects alveolar and jaw bone formation during the growth period.

    • Author(s)
      Shimomoto Y.
    • Journal Title

      Journal of Japanese Society of Bone Morphometry (In press)

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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