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Elucidation of dental root resorption and its restoration, induced by orthodontic force ; Application of mechanical stress to epithelial cells derived of dental lamina

Research Project

Project/Area Number 16592057
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionKanagawa Dental College

Principal Investigator

YAMAUCHI Masato  Kanagawa Dental College, Dentistry, Orthodontics, Assistant professor (30230311)

Co-Investigator(Kenkyū-buntansha) TSUNODA Akira  Kanagawa Dental College, Dentistry, Endodontics, Lecturer (70236933)
KOBAYASHI Masaru  Kanagawa Dental College, Dentistry, Oral Surgeon, Lecturer (00162024)
HATA Ryu-ichiro  Kanagawa Dental College, Dentistry, Oral Biochemistry, Professor (10014276)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordscementum / dental root resorption / mechanical stress / amelogenin / bone sialoprotein / promoter / transcription / セメント芽細胞 / Bone sialoprotein / 遺伝子組み換え蛋白質 / セメント芽細胞前駆体細胞 / 歯堤由来上皮細胞 / 変異体蛋白質 / cDNAライブラリー / 物理的外力 / 歯牙移動モデル / 有限要素解析 / 培養細胞伸展システム
Research Abstract

Cementblast precursor cell could induce osteoclast and could produce its matrix to restore on the dental root resorption lacunae, which regulated by amelogenin or its variants. Bone sialoprotein (BSP) is sialic acid-rich glycophosphoprotein that is abundant in surface of dental root, likewise trabecular bone. It was conceivable that BSP participate with the defense mechanism against dental root resorption and restoration. Orthodontic forces might be a determination factor on the dental root resorption, which was involved in the cellular events. To elucidate the functional roles of BSP in cementblast precursor cell on the dental root resorption, we examined the effects of purified amelogenin as well as cyclic stretch on its gene expression.
RT-PCR analyses revealed that 1.0 μg amelogenin purified from immature enamel of bovine tooth could induced a 1.6 fold increase of BSP mRNA expression in mouse immortalized dental follicle progenitor cells, MDFE6Δ127. Application of Cyclic stretch (6% … More , 1Hz) caused approximately 2.0 fold enhancement of BSP mRNA expression in MDFE6Δ127. No synergistically effects between addition of amelogenin and application of cyclic stretch were observed in MDFE6Δ127. Transcription analyses revealed amelogenin increased a 4 fold of promoter activity in pLUC9.0kb, 1.5 fold of in pLUC5.2kb, whereas any enhancement was not observed in pLUC0.8kb. It was presumed that amelogenin response ' cis-element was existed in the upstream promoter between nts -5394 and-9254 lesion within BSP gene. In contrast, 3.2 fold increase of transcription was observed in pLUC5.2kb induced by application of cyclic stretch. Moreover, relative enhancement of promoter activities has been shown in both pLUC9.0kb and pLUC0.8kb with cyclic stretch. We assumed that broad distribution of cyclic stretch response cis-elements of which activity was peaked around nts-5394 legion were existed in the transcription regulatory gene of BSP.
Taken together, these results suggest that amelogenin could cause enhancement of BSP transcription and its gene expression in MDFE6Δ127, which had distinct reaction pathways compared with the induction of cyclic stretch. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (37 results)

All 2007 2006 2005 2004

All Journal Article (37 results)

  • [Journal Article] Type III collagen is essential for growth acceleration of human osteoblastic cells by ascorbic acid 2-phosphate,a long-acting vitamin C delivative2007

    • Author(s)
      Maehata Y.
    • Journal Title

      Matrix Biol. 26・5

      Pages: 371-381

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Type III collagen is essential for growth acceleration of human osteoblastic cells by ascorbic acid 2-phosphate, a long-acting vitamin C delivative2007

    • Author(s)
      Maehata Y.
    • Journal Title

      Matrix Biol. 26-5

      Pages: 371-381

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Type IIl collagen is essential for growth acceleration of human osteoblastic cells by ascorbic acid 2-phosphate, a long-acting vitamin C delivative2007

    • Author(s)
      Maehata Y
    • Journal Title

      Matrix Biol

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Both direct and collagen-mediated signals are required for active VitaminD(3)elicted differentiation of human osteoblastic cell;Roles of osterix,an osteoblast-related transcription factor2006

    • Author(s)
      Maehata Y.
    • Journal Title

      Matrix Biol. 25・1

      Pages: 47-58

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] BRAK/CXCL14 expression suppresses tumor growth in vivo in human oral carcinoma cells2006

    • Author(s)
      Ozawa S
    • Journal Title

      Biochem Biophys Res Commun. 348・2

      Pages: 406-412

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Both direct and collagen-mediated signals are required fof active2006

    • Author(s)
      Maehata Y.
    • Journal Title

      Matrix Biol. 25-1

      Pages: 47-58

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] BRAK/CXCL14 expression suppresses tumor growth in vivo in human oral carcinoma cells2006

    • Author(s)
      Ozawa S.
    • Journal Title

      Biochem Biophys Res Commun. 348-2

      Pages: 406-412

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] BRAK/CXCL14 expression suppresses tumor growth in vivo in human oral carcinoma cells2006

    • Author(s)
      Ozawa S
    • Journal Title

      Biochem Biophys Res Commun 348・2

      Pages: 406-12

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Both direct and collagen-mediated signals are required for active Vitamin D (3) elicted differentiation of human osteoblastic cell ; Roles of osterix, an osteoblast-related transcription factor.2006

    • Author(s)
      Maehata Y
    • Journal Title

      Matrix Biol 25・1

      Pages: 47-58

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Acidic extracellular pH induces matrix metalloproteinase-9 expression in mouse metastatic melanoma cells through the phospholipase D-MAPKs signaling2005

    • Author(s)
      Kato Y.
    • Journal Title

      J Biol.Chem. 280・12

      Pages: 10938-44

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] An in vivo model to study osteogenic gene regulation:targeting an avian receptor(TVA)to bone with the bone sialoprotein(BSP)promoter2005

    • Author(s)
      Li L.
    • Journal Title

      J Bone Miner Res. 20・8

      Pages: 1403-13

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Tissue-and bone cell-specific expression of bone sialoprotein is directed by a 9.0kb promoter in transgenic mice2005

    • Author(s)
      Paz J.
    • Journal Title

      Matrix Biol. 24・5

      Pages: 341-52

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation and functional analysis of baculovirus-expressed recombinant human osteopontin2005

    • Author(s)
      Horiguchi M.
    • Journal Title

      Bull.Kanagawa Dent.Col. 33・2

      Pages: 61-70

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Reduced excision repair cross-complementing 1 expression associates with enhanced papilloma formation and fibroblast transformation after genetic disruption of secreted protein acidic and rich in cysteine2005

    • Author(s)
      Kato Y.
    • Journal Title

      Int J Oncol. 27・3

      Pages: 759-68

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Acidic extracellular pH induces matrix metalloproteinase-9 expression in mouse metastatic melanoma cells through the phospholipase D-MAPKs signaling.2005

    • Author(s)
      Kato Y.
    • Journal Title

      J Biol. Chem. 280-12

      Pages: 10938-44

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] An in vivo model to study osteogenic gene regulation : targeting an avian receptor(TVA) to bone with the bone sialoprotein(BSP) promoter.2005

    • Author(s)
      Li L.
    • Journal Title

      J Bone Miner Res 20-8

      Pages: 1403-13

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Tissue-and bone cell-specific expression of bone sialoprotein is directed by a 9.0kb promoter in transgenic mice.2005

    • Author(s)
      Paz J.
    • Journal Title

      Matrix Biol 24-5

      Pages: 341-52

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation and functional analysis of baculovirus-expressed recombinant human osteopontin.2005

    • Author(s)
      Horiguchi M.
    • Journal Title

      Bull. Kanagawa Dent. Col. 33-2

      Pages: 61-70

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Reduced excision repair cross-complementing 1 expression associates with enhanced papilloma formation and fibroblast transformation and fibroblast transformation after genetic diruption of secreted protein acidic and rich in cysteine2005

    • Author(s)
      Kato Y.
    • Journal Title

      Int J Oncol. 27-3

      Pages: 759-68

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] An in vivo model to study osteogenic gene regulation : targeting an avian receptor (TVA) to bone with the bone sialoprotein (BSP) promoter.2005

    • Author(s)
      Lin L
    • Journal Title

      J bone Miner Res 20・8

      Pages: 1403-1413

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Tissue-and bone cell-specific expression of bone sialoprotein is directed by a 9.0kb promoter in transgenic mice.2005

    • Author(s)
      Paz J
    • Journal Title

      Matrix Biol 24・5

      Pages: 341-352

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Isolation and functional analysis of baculovirus-expressed recombinant human osteopontin.2005

    • Author(s)
      Horiguchi M
    • Journal Title

      Bull.Kanagawa Dent.Col. 33・2

      Pages: 61-70

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Reduced excision repair cross-complementing 1 expression associates with enhanced papilloma formation and fibroblast transformation after genetic disruption of secreted protein acidic and rich in cysteine2005

    • Author(s)
      Kato Y
    • Journal Title

      Int J Oncol. 27・3

      Pages: 759-768

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of SPARC in tongue carcinoma of stage II is associated with poor prognosis ; an immunohistochemical study of 86 cases.2005

    • Author(s)
      Kato Y
    • Journal Title

      Int J Mol Med. 16・2

      Pages: 263-268

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Acidic extracellular pH induces matrix metalloproteinase-9 expression in mouse metastatic melanoma cells through the phospholipase D-MAPKs signaling.2005

    • Author(s)
      Kato Y
    • Journal Title

      J Biol Chem (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Autoregulation of bone sialoprotein gene in pre-osteoblastic and non-osteoblastic cells2004

    • Author(s)
      Tu Q.
    • Journal Title

      Biochem Biophys Res Commun. 316・2

      Pages: 461-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Inostamycin suppresses vascular endothelial growth factor-stimulated growth and migration of human umbilical vein endothelial cells2004

    • Author(s)
      Baba Y.
    • Journal Title

      Clin Exp Metastasis 21・5

      Pages: 419-25

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of ascorbic acid and ascorbic acid 2-phosphate,a long-acting vitamin C derivative,on the proliferation and differentiation of human osteoblast-like cells2004

    • Author(s)
      Takamizawa S.
    • Journal Title

      Cell Biol Int. 28・4

      Pages: 255-65

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Rare autosomal recessive cardiac valuvar form of Ehleres-Dannos syndrome results from mutations in the COLIA2 gene that activate the nonsense-mediated RNA decay pathway2004

    • Author(s)
      Schwarze U.
    • Journal Title

      Am J Hum Genet. 74・5

      Pages: 917-30

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Autoregulation of bone sialoprotein gene in pre-osteoblastic and non-osteoblastic cells2004

    • Author(s)
      Tu Q.
    • Journal Title

      Biochem Biophys Res Commun. 316-2

      Pages: 461-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Inostamycin suppresses vascular endothelial growth factor-stimulated growth and migration of human umbilical vein endothelial cells2004

    • Author(s)
      Baba Y.
    • Journal Title

      Clin Exp Metastasis 21-5

      Pages: 419-25

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of ascorbic acid and ascorbic acid 2-phosphate, a long-acting vitamin C derivative, on the proliferation and differentiation of human osteoblast-like cells.2004

    • Author(s)
      Takamizawa S.
    • Journal Title

      Cell Biol Int 28-4

      Pages: 255-65

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Rare autosomal recessive cardiac valuvar form of Ehleres-Dannos syndrome results from mutations in the COLIA2 gene that activate the nonsense-mediated RNA decay pathway.2004

    • Author(s)
      Schwarze U.
    • Journal Title

      Am J Hum Genet 74-5

      Pages: 917-30

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Autoregulation of bone sialoprotein gene in pre-osteoblastic and non-osteoblastic cells2004

    • Author(s)
      Tu Q
    • Journal Title

      Biochem Biophys Res Commun 316・2

      Pages: 461-467

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inostamycin suppresses vascular endothelial growth factor-stimulated growth and migration of human umbilical vein endothelial cells2004

    • Author(s)
      Baba Y
    • Journal Title

      Clin Exp Metastasis 21・5

      Pages: 419-425

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of ascorbic acid and ascorbic acid 2-phosphate, a long-acting vitamin C derivative, on the proliferation and differentiation of human osteoblast-like cells2004

    • Author(s)
      Takamizawa S
    • Journal Title

      Cell Biol Int. 28・4

      Pages: 255-265

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Rare autosomal recessive cardiac valvular form of Ehlers-Danlos syndrome results from mutations in the COL1A2 gene that activate the nonsense-mediated RNA decay pathway.2004

    • Author(s)
      Schwarze U
    • Journal Title

      Am J Hum Genet. 74・5

      Pages: 917-930

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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