| Project/Area Number |
16592059
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Aichi-Gakuin University |
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Principal Investigator |
TSUCHIYA Tomoyuki Aichi-Gakuin University, School of Dentistry, Professor, 歯学部, 教授 (20090144)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANO Takashi Aichi-Gakuin University, School of Dentistry, Assistant Professor, 歯学部, 講師 (20319205)
HIGASHI Kimihiko Aichi-Gakuin University, School of Dentistry, Assistant Professor, 歯学部, 講師 (80367622)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | fluoride / plaque / mineral / salivary clearance |
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| Research Abstract |
The aim of this study was to examine the influence of salivary clearance on the fluoride and mineral distributions in the plaque in vivo. Salivary clearances at the buccal surface of the lateral incisor and the first molar in the upper jaw were determined using the fluoride mouthrinse-dilution technique. Eight male volunteer (20-23years) were asked to rinse with 20ml (225ppmF) of NaF solution for 30 seconds. Ten minutes after the rinse, saliva samples were collected from both tooth surfaces using paper points. Fluoride concentrations analyzed were used as the indicator of the salivary clearance.
Four plaque generators were bonded on both buccal surfaces of the incisor and the molar. One week after the plaque was formed, two generators of one side were firstly removed, the remaining two generators were then collected 10 minutes after fluoride mouth-rinse (20ml,30 seconds). Fluoride and mineral (calcium and phosphorus) distributions in plaque were analyzed using a micro-slicing method. Salivary fluoride concentrations (11.2 ppm) at the molar were lower than those (24.2 ppm) at the incisor (p<0.05).
Changes in the plaque fluoride profiles before and after the mouth-rinse showed rapid uptaking of fluoride into the plaque and fluoride being diffused throughout the plaque. Ten minutes after exposing to the rinse, fluoride concentrations in the all layers except inner layer (8th) of plaque on the molar were significantly lower than those of the incisor. No significant differences was found in the mineral distribution determined.
It was concluded that fluoride could easily penetrate into the plaque deposited with fluoride mouth-rinse and plaque fluoride decrease at the location of higher salivary clearance in vivo.
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