| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Research Abstract |
1.Yields and analyses of DNA obtained from Finger-contacted dental materials
Finger-contacted resin pieces were prepared by compressing resin pieces from both sides with the forefingers (n=20). Palladium (n=20) and silver alloy pieces (n=20) were also prepared using the same method. More than 10 ng of DNA was obtained from half of the sample pieces, however, the amounts of extractable DNA did not correlate with the kinds of materials, and a period of time of finger contacted and compressed time.
The samples from which more than 10 ng of DNA was obtained were subjected to sex determination by PCR of the segments of the amelogenin gene, VNTR analysis at the D4S43 locus, and STR analysis at CSF1PO, TPOX, and TH01 locus. All of these analyses were successful for all of the samples tested.
2.DNA analysis of the samples which less than 10 ng of DNA was obtained
Direct sequence analysis on mtDNA (HV1 region) of the samples providing less than 10 ng of DNA (n=8), was performed. As the result, while sequence analysis failed to find the contaminator's sequence, the experimenter's sequence was found in all samples.
3.Sex determination and VNTR analysis using samples which contaminated by a female and male's finger
In the sex determination, all samples were judged male, and in the VNTR analysis, only ladder bands were detected.
4.Yields of DNA obtained from collected resin prostheses
0.5×0.5×0.1 cm pieces were prepared from collected resin prostheses used by patients (n=55). The amounts of DNA extracted were significantly different among the samples and ranged from about 40 ng to 1.5μg.
These results indicate that the dental materials are a good source of DNA for personal identification ; however, finger contact will be significant effect, that is contaminated with over 10 ng of DNA. Therefore sequencing analyses are essential for forensic samples suspected contamination.
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