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Physiological roles of apoptotic cell clearance

Research Project

Project/Area Number 16601004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 細胞死(アポトーシス)
Research InstitutionRIKEN

Principal Investigator

TANAKA Masato  RIKEN, Laboratory for Innate Cellular Immunity, Laboratory Head, 自然免疫研究チーム, チームリーダー (00294059)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsmacrophage / apoptosis / phagocytosis / autoantibody / type II epithelial cells / ARDS / 死細胞 / 貪食 / MFG-E8
Research Abstract

Milk fat globule-EGF-factor 8 (MFG-E8), secreted by activated macrophages and immature dendritic cells, links apoptotic cells and phagocytes, and promotes phagocytosis of apoptotic cells. We found that an MFG-E8 mutant, designated as D89E, carrying a point mutation in an RGD motif, inhibited the phagocytosis of apoptotic cells by a wide variety of phagocytes. When intravenously injected into mice, the D89E protein induced the production of auto-antibodies including anti-phospholipids antibodies and anti-nuclear antibodies. The production of auto-antibodies was enhanced by the co-injection of syngeneic apoptotic thymocytes. Following the induction of auto-antibody production by D89E, the treated mice showed a long-term elevation of the titer for auto-antibodies, and developed IgG deposition in the gromeruli. These results indicated that the impairment of apoptotic cell phagocytosis led to auto-antibody production.
We generated transgenic mice in which type II lung epithelial cells as well as macrophages were transiently ablated. Human diphtheria toxin receptor was expressed under the control of the lysozyme M gene promoter in the mice. When diphtheria toxin was administrated to the mice, they suffered from acute lung injury, and died within 4 days. Type II cells as well as alveolar macrophages were deleted in the mice treated with diphtheria toxin. The amount of surfactant proteins A and B in bronchoalveolar lavage fluid was greatly reduced in the diphtheria toxin-treated transgenic mice. When the bone marrow from wild-type mice was transplanted into the irradiated transgenic mice, the alveolar macrophages became resistant to diphtheria toxin, but the mice still suffered from acute lung injury. These results indicated that ablation of type II cells caused lethal acute respiratory distress in association with the reduced amount of surfactant proteins.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (14 results)

All 2006 2005 2004 Other

All Journal Article (14 results)

  • [Journal Article] Opposite effects of family GTPases on engulfment of apoptotic cells by macrophages.2006

    • Author(s)
      Nakaya, M.
    • Journal Title

      The Journal of Biological Chemistry 281

      Pages: 8836-8842

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Opposite effects of Rho family GTPases on engulfment of apoptotic cells by macrophages.2006

    • Author(s)
      Nakaya, M.
    • Journal Title

      The Journal of Biological Chemistry (In press.)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Apoptotic cell clearance by phagocytes.2005

    • Author(s)
      Tanaka, M.
    • Journal Title

      International Congress Sreies 1285

      Pages: 55-59

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Apoptotic cell clearance by phagocytes.2005

    • Author(s)
      Tanaka, M.
    • Journal Title

      International Congress Series 1285

      Pages: 55-59

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Masking of phosphatidylserine inhibits apoptotic cell engulfment and induces autoantibody production in mice.2004

    • Author(s)
      Asano, K.
    • Journal Title

      The Journal of Experimental Medicine 200

      Pages: 459-467

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice.2004

    • Author(s)
      Hanayama, R.
    • Journal Title

      Science 304

      Pages: 1147-1150

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Expression of milk far globule epidermal growth factor 8 in immature dendritic cells for engulfment of apoptotic cells.2004

    • Author(s)
      Miyasaka, K.
    • Journal Title

      European Journal of Immunology 34

      Pages: 1414-1422

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of Developmental Endothelial Locus-1 in a Subset of Macrophages for Engulfment of Apoptotic Cells.2004

    • Author(s)
      Hanayama, R.
    • Journal Title

      The Journal of Immunology 172

      Pages: 3876-3882

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Masking of phosphatidylserine inhibits apoptotic cell engulfment and induces autoantibody production in mice.2004

    • Author(s)
      Asano, K., M.Miwa, K.Miwa, R.Hanayama, H.Nagase, S.Nagata, M.Tanaka
    • Journal Title

      J.Exp.Med. 200,(4)

      Pages: 459-467

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice.2004

    • Author(s)
      Hanayama, R., M.Tanaka, K.Miyasaka, K.Aozasa, M.Koike, Y.Uchiyama, S.Nagata
    • Journal Title

      Science 304

      Pages: 1147-1150

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of milk fat globule epidermal growth factor 8 in immature dendritic cells for engulfment of apoptotic cells.2004

    • Author(s)
      Miyasaka, K., R.Hanayama, M.Tanaka, S.Nagata
    • Journal Title

      Eur.J.Immunol. 34

      Pages: 1414-1422

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of Developmental Endothelial Locus-1 in a Subset of Macrophages for Engulfment of Apoptotic Cells.2004

    • Author(s)
      Hanayama, R., M.Tanaka, K.Miyasaka, K.Aozasa, M.Koike, Y.Uchiyama, S.Nagata
    • Journal Title

      J.Immunol. 172

      Pages: 3876-3882

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression of milk fat globule epidermal growth factor 8 in immature dendritic cells for engulfment of apoptotic cells.2004

    • Author(s)
      Miyasaka, K.
    • Journal Title

      European Journal of Immunology 34

      Pages: 1414-1422

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Opposite effects of Rho family GTPases on engulfment of apoptotic cells by macrophages.

    • Author(s)
      Nakaya, M., M.Tanaka, Y.Okabe, R.Hanayama, S.Nagata
    • Journal Title

      J.Biol.Chem. 281,(13)

      Pages: 8836-8842

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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