| Project/Area Number |
16613010
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食の安全
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| Research Institution | Rakuno Gakuen University |
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Principal Investigator |
YOKOTA Hiroshi Rakuno Gakuen University, School of Veterinary Medicine, Professor, 獣医学部, 教授 (90137414)
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| Co-Investigator(Kenkyū-buntansha) |
DOH-URA Katsumi Tohoku University, School of Medicine, professor, 医学部, 教授 (00263012)
MORI Seiichi Hokkaido Animal Research Center, Cheaf of Center, 場長 (10390824)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Scrapie / BSE / Serum Proteins / 2d-electrophoresis / Diagnosis / 血液診断 / タンパク質 / 尿 |
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| Research Abstract |
The objective of this study is to find signals of mouse scrapie and BSE by comprehensive analysis of serum components. Several proteins were found to detect only in the sera from scrapie mice by 2-dementional electrophoresis. The detected proteins were analyzed by Ms/Ms and one of them was identified to antibodies against Fas protein expressed on the cell surface membrane. In BSE sera which were obtained from the Weybridge Veterinary Laboratory, several protein bands recognized by serum antibodies were found by Western blotting analysis. The antigens were extracted from the protein stops prepared by 2-clementional electrophoresis, and were analyzed by Ms.
These data indicate that autoantibodies were produced in the sera of not only scrapie mice but also BSE cows. In next study, we can consider to use these serum components diagnostic signals.
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