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タンパク質の多様性獲得戦略の解明に基づいたゲノム情報の解読

Research Project

Project/Area Number 16637005
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section企画調査
Research Field Biophysics
Research InstitutionNagahama Institute of Bio-Science and Technology

Principal Investigator

郷 通子  長浜バイオ大学, バイオサイエンス学部, 教授 (70037290)

Co-Investigator(Kenkyū-buntansha) 高橋 健一  長浜バイオ大学, バイオサイエンス学部, 助教授 (20322737)
加藤 晃一  名古屋市立大学, 大学院・薬学研究科, 教授 (20211849)
鈴木 利治  北海道大学, 大学院・薬学研究科, 教授 (80179233)
古川 清  財団法人東京都高齢者研究, 福祉振興財団, 副参事研究員 (10190133)
由良 敬  日本原子力研究所, 計算科学技術推進センター, 副主任研究員 (50252226)
Project Period (FY) 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥3,500,000 (Direct Cost: ¥3,500,000)
Keywordsタンパク質の多様化 / ゲノム情報 / 選択的スプライシング / RNA編集 / 翻訳後修飾 / 選択的プロテオリシス / プロテインスプライシング / 抗体の多様性獲得
Research Abstract

生物がゲノムに内包された限られた数の遺伝子を駆使して天文学的ともいえるタンパク質の多様性を獲得している戦略の全体像を俯瞰的に捉えることは、ゲノム情報を読み解くうえで極めて重要である。そこで、以下のような企画調査を行い、タンパク質多様性獲得戦略に関するこれまでの研究と今後の展開に対する指針を検討した。
2回の班会議の開催(7月16-17日、長浜;12月4日、東京)、3回の公開シンポジウムの開催(第27回日本分子生物学会年会ワークショップW3E「タンパク質の多様性獲得戦略」、12月10日、神戸;「選択的スプライシングによる多様性の創造」、12月4日、東京;「Strategies for the acquirement of functional diversity of proteins(タンパク質の多様性は限られたゲノム情報からいかにして生み出されるか?)」、1月20日、東京)、5名の海外研究者の招聘(Adrian R.Krainer(Cold Spring Harbor Laboratory)、E.Yvonne Jones(University of Oxford)、Claudio Joazeiro(Genomics Institute of the Novartis Research Foundation)、Michiko N.Fukuda(The Burnham Institute)、Jianxin Bao(Washington University))。
これらの活動を通して得た指針に基づき、タンパク質多様性獲得戦略の全体像把握に向けた研究を推進していくために、科学研究費補助金「特定領域研究」の平成17年度新規発足申請(申請領域名「タンパク質機能の多様性獲得戦略」)を行った。

Report

(1 results)
  • 2004 Annual Research Report
  • Research Products

    (52 results)

All 2005 2004

All Journal Article (50 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Rice nuclear genome continuously integrates, shuffles and eliminates the chloroplast genome to cause chloroplast-nuclear DNA flux.2005

    • Author(s)
      Matsuo, M., Ito, Y., Yamauchi, R., Obokata, J.
    • Journal Title

      The Plant Cell 17

      Pages: 665-675

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Myristoyl moiety of HIV Nef is involved in regulation of the interaction with calmodulin in vivo2005

    • Author(s)
      Matsubara, M., Jing, T., Kawamura, K., Shimojo, N., Titani, K., Hashimoto, K., Hayashi, N.
    • Journal Title

      Protein Sci. 14

      Pages: 494-503

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Deletion of N-terminus of human tyrosine hydroxylase type 1 enhances stability of the enzyme in ArT-20 cells.2005

    • Author(s)
      Nakashima, A., Hayashi, N., Kaneko, YS., Mori, K., Egusa, H., Nagatsu, T., Ota, A.
    • Journal Title

      J.Neurosci.Res. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mouse Na/K-ATPase β1-subunit has a K-dependent cell adhesion activity for β-GlcNAc-terminating glycans.2005

    • Author(s)
      Kitamura, N., et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effect of Staurosporine on N-glycosylation and cell adhesion to fibronectin of SW480 human colorectal adenocarcinoma cells.2005

    • Author(s)
      Sato, T., et al.
    • Journal Title

      Eur.J.Pharamceu. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] β-1, 4-galactosyltransferase III is involved in the biosynthesis of highly branched N-linked sugars in WI38 human lung embryonic fibroblastic cells.2005

    • Author(s)
      Sato, T., et al.
    • Journal Title

      Res.Commun.Biochem.Cell & Molec.Biol. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Newly sequences eRF1s from ciliates : the diversity of stop codon usage and the molecular surfaces that are important for stop codon interactions2005

    • Author(s)
      Kim, T.P.O., et al.
    • Journal Title

      Gene 346

      Pages: 277-286

    • Related Report
      2004 Annual Research Report
  • [Journal Article] タンパク質のフォールディングー自己組織化と遺伝子のスプライシング2005

    • Author(s)
      郷 通子, 由良 敬
    • Journal Title

      Bionics 2・1

      Pages: 38-43

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular mechanism of vde-intiated intein homing in yeast nuclear genome2004

    • Author(s)
      Fukuda T, Nagai Y, Ohya Y
    • Journal Title

      Adv Biophys. 38

      Pages: 215-232

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A site-specific factor interacts directly with its cognate RNA editing site in chloroplast transcripts.2004

    • Author(s)
      Miyamoto, T., Obokata, J., Sugiura, M.
    • Journal Title

      Proceedings of the National academy of Sciences of the United States of America 101

      Pages: 48-52

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vitro evolution of translational regulatory cis-elements.2004

    • Author(s)
      Nagao, I., Masuyama, K., Obokata, J.
    • Journal Title

      Endocytobiosis and Cell Research 15

      Pages: 385-389

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Core promoter diversity and photosynthesis gene regulation.2004

    • Author(s)
      Nakamura, M., Tsunoda, T., Yoshitsugu, T., Obokata, J.
    • Journal Title

      Endocytobiosis and Cell Research 15

      Pages: 300-308

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vitro editing system from higher plant chloroplasts.2004

    • Author(s)
      Hirose, T., Miyamoto, T., Obokata, J., Sugiura, M.
    • Journal Title

      Methods in Molecular Biology 265

      Pages: 333-344

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Discrimination of isomeric fragment ions observed in tandem mass spectra of biantennary oligosaccharides by use of selective isotope labeling2004

    • Author(s)
      K.Kato et al.
    • Journal Title

      J.Mass Spectrom.Soc.Japan 52

      Pages: 284-288

    • NAID

      10013736894

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ユビキチンリガーゼの機能発現の構造的基盤2004

    • Author(s)
      坂田絵里
    • Journal Title

      細胞工学 23

      Pages: 825-831

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Temperature-dependent isologous Fab-Fab interaction that mediates cryocrystallization of a monoclonal immunoglobulin G2004

    • Author(s)
      H.Yagi et al.
    • Journal Title

      Mol.Immunol. 41

      Pages: 1211-1215

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Gentamicin binds to the lectin site of calreticulin and inhibits its chaperone activity2004

    • Author(s)
      T.Horibe et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 323

      Pages: 281-287

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Recombinant curculin heterodimer exhibits taste-modifying and sweet-tasting activities2004

    • Author(s)
      M.Suzuki et al.
    • Journal Title

      FEBS Lett. 573

      Pages: 135-138

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Structural basis of sugar-recognizing ubiquitin ligase2004

    • Author(s)
      T.Mizushima et al.
    • Journal Title

      Nature Struct.Mol.Biol. 11

      Pages: 365-370

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Modulation of amyloid precursor protein cleavage by cellular Sphingolipids.2004

    • Author(s)
      Sawamura, N.et al.
    • Journal Title

      J.Biol.Chem 279

      Pages: 11984-11991

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Facilitation of stress-induced phosphorylation of β-amyloid precursor protein family members by X11-like/Mint2 protein2004

    • Author(s)
      Taru, H., Suzuki, T.
    • Journal Title

      J.Biol.Chem 279

      Pages: 21628-21636

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Coordinated metabolism of Alcadein and amyloid β-protein precursor regulated FE65-dependent gene transactivation.2004

    • Author(s)
      Araki, Y., et al.
    • Journal Title

      J.Biol.Chem 279

      Pages: 24343-24354

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The APP intracellular domain forms nuclear multiprotein complexes and regulates the transcription of its own precursor2004

    • Author(s)
      von Rotz, R.C., et al.
    • Journal Title

      J.Cell Sci. 117

      Pages: 4435-4448

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Caspase cleavage of β-amyloid precursor protein is regulated by cytoplasmic phosphorylation.2004

    • Author(s)
      Taru, H., et al.
    • Journal Title

      FEBS Lett. 567

      Pages: 248-252

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The endoplasmic reticulum stress response is stimulated through the continuous activation of transcription factors ATF6 and XBP1 in Ins2+/Akita pancreatic βcells.2004

    • Author(s)
      J.NOZAKI et al.
    • Journal Title

      Genes Cells 9

      Pages: 261-270

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Advanced glycation end products increase collagen-specific chaperone protein in mouse diabetic nephropathy.2004

    • Author(s)
      S.OHASHI et al.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 19816-19823

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of multiple members of the HSP70 family in platyfish culture cells : molecular evolution of stress protein HSP70 in vertebrates.2004

    • Author(s)
      M.YAMASHITA et al.
    • Journal Title

      Gene 336

      Pages: 207-218

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Insufficient folding of type IV collagen nad formation of abnormal basement membrane-like structure in embryoid bodies derived from HSP47-null ES cells.2004

    • Author(s)
      Y.MATSUOKA et al.
    • Journal Title

      Mol.Biol.Cell. 15

      Pages: 4467-4475

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Accumulation of type IV collagen in dilated endoplasmic reticulum leads to apoptosis HSP47-Knockout mouse embryos through the induction of CHOP.2004

    • Author(s)
      T.MARUTANI et al.
    • Journal Title

      J.Cell.Sci. 117

      Pages: 5913-5922

    • Related Report
      2004 Annual Research Report
  • [Journal Article] CHOP induces death by promoting protein synthesis and oxidation in the stressded endoplasmic reticulum2004

    • Author(s)
      S.J.MARCINIAK et al.
    • Journal Title

      Genes Develop 18

      Pages: 3066-3077

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Myristoylation-regulated direct interaction between calcium-bound calmodulin and N-terminal region of pp60^<v-src">2004

    • Author(s)
      Hayashi, N., Nakagawa, C., Takasaki, A., Jinbo, Y., Yamakawa, Y., Titani, K., Hashimoto, K., Izumi, Y., Matsushima, N.
    • Journal Title

      J.Mol.Biol. 338

      Pages: 169-180

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Transcriptional regulation of human β-1,4-galactosyltransferase V gene in cancer cells : Essential role of transcription factor Sp 1.2004

    • Author(s)
      Sato, T., Furukawa, K.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 39574-39583

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Suppression of the proliferation and neurite extension of human neuroblas-toma cells on immobilized Psathyrella velutina lectin.2004

    • Author(s)
      Kitamura, N., et al.
    • Journal Title

      J.Neurosci.Res. 75・3

      Pages: 384-390

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Rapid cell senescence-associated changes in galactosylation of N-linked oligosaccharides in human lung adenocarcinoma A549 cells.2004

    • Author(s)
      Kawado, T., et al.
    • Journal Title

      Arch.Biochem.Biophy. 426

      Pages: 306-313

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Activation of mammalian unfolded protein response is compatible with2004

    • Author(s)
      S.Nadanaka, et al.
    • Journal Title

      Mol.Biol.Cell 15

      Pages: 2537-2548

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Differential contributions of ATF6 and XBP1 to the activation of endoplasmic reticulum stress-responsive cis-acting elements ERSE, UPRE and ERSE-II.2004

    • Author(s)
      K.Yamamoto et al.
    • Journal Title

      J.Biochem. 136

      Pages: 343-350

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Activating transcription factor 6 modulates the sterol regulatory2004

    • Author(s)
      L.Zeng et al.
    • Journal Title

      EMBO J. 23

      Pages: 950-958

    • Related Report
      2004 Annual Research Report
  • [Journal Article] XBP1:a link between the unfolded protein response, lipid biosynthesis and biogenesis of the endoplasmic reticulum.2004

    • Author(s)
      R.Sriburi et al.
    • Journal Title

      J.Cell.Biol. 167

      Pages: 35-41

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Hepatitis C virus suppresses the IRE1-XBP1 pathway of the unfolded protein response.2004

    • Author(s)
      K.D.Tardif et al.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 17158-17164

    • Related Report
      2004 Annual Research Report
  • [Journal Article] XBP1 is essential for survival under hypoxic conditions and is required for tumor growth.2004

    • Author(s)
      L.Romero-Ramirez et al.
    • Journal Title

      Cancer Res. 64

      Pages: 5943-5947

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A novel protein-conjugating system for Ufm1, a ubiquitin-fold modifier.2004

    • Author(s)
      Komatsu M, Chiba T, Tatsumi K, Iemura S, Tanida I, Okazaki N, Ueno T, Kominami E, Natsume T, Tanaka
    • Journal Title

      EMBO J. 23

      Pages: 1977-1986

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Structural basis of sugar-recognizing ubiquitin ligase.2004

    • Author(s)
      Mizushima T, Hirao T, Yoshida Y, Lee SJ, Chiba T, Iwai K, Yamaguchi Y, Kato K, Tsukihara T, Tanaka K.
    • Journal Title

      Nat.Struct.Mol.Biol. 11

      Pages: 365-370

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Ligand-dependent switching of ubiquitin-proteasome pathways for estrogen receptor.2004

    • Author(s)
      Tateishi Y, Kawabe Y, Chiba T, Murata S, Ichikawa K, Murayama A, Tanaka K, Baba T, Kato S, Yanagisawa J.
    • Journal Title

      EMBO J. 23

      Pages: 4813-4823

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Oxidative stress sensor Keap1 functions as an adaptor for Cul3-based E3 ligase to regulate proteasomal degradation of Nrf2.2004

    • Author(s)
      Kobayashi A, Kang MI, Okawa H, Ohtsuji M, Zenke Y, Chiba T, Igarashi K, Yamamoto M.
    • Journal Title

      Mol.Cell Biol. 24

      Pages: 7130-7139

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Ubiquitin-fusion degradation pathway plays an indispensable role in naked DNA vaccination with a chimeric gene encoding a syngeneic cytotoxic T lymphocyte epitope of melanocyte and green fluorescent protein2004

    • Author(s)
      Zhang M, Ishii K, Hisaeda H, Murata S, Chiba T, Tanaka K, Li Y, Obata C, Furue M, Himeno K.
    • Journal Title

      Immunology 112

      Pages: 567-574

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The fusion oncoprotein PML-PAR induces endoplasmic reticulum-associated degradation of N-CoR and ER stress.2004

    • Author(s)
      Khan MM, Nomura T, Chiba T, Tanaka K, Yoshida H, Mori K, Ishii S.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 11814-11824

    • Related Report
      2004 Annual Research Report
  • [Journal Article] History dependent effects on phenotypic expression of a newly emerged gene2004

    • Author(s)
      Suzuki, T., Kashiwagi, A., Urabe, I., Yomo, T.
    • Journal Title

      Biosystems 77(1-3)

      Pages: 137-141

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of a cascading genetic network within liposomes2004

    • Author(s)
      Ishikawa, K., Sato, K., Shima, Y., Urabe, I., Yomo, T.
    • Journal Title

      FEBS Lett. 576(3)

      Pages: 387-390

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 表現形質としてのたんぱく質とその進化2004

    • Author(s)
      郷通子
    • Journal Title

      科学(岩波書店) 第74巻

      Pages: 1240-1245

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Role of KaiC phosphorylation in the circadian clock system of Synechoccus elongatus PCC 79422004

    • Author(s)
      Nishiwaki T, Satomi Y, Nakajima M, Lee C, Kiyohara R, Kageyama H, Kitayama Y, Temamoto M, Yamaguchi A, Hijikata A, Go M, Iwasaki H, Takao T, Kondo
    • Journal Title

      Proc Natl Acad Sci U S A 101(38)

      Pages: 13927-13932

    • Related Report
      2004 Annual Research Report
  • [Book] Topics in Current Chemistry : Collagen (Eds Brinckmann et al.) Collagen Biosynthesis2005

    • Author(s)
      T.KOIDE et al.
    • Publisher
      Springer-Veriag(In Press)
    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] 抗IgSF4抗体及びその利用2005

    • Inventor(s)
      林 et al.
    • Industrial Property Rights Holder
      株式会社抗体研究所
    • Filing Date
      2005-02-28
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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