らせん多糖を足場としたDNAモノマーの自己配列と複製

• Project/Area Number: 16655048
• Research Category: Grant-in-Aid for Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Polymer chemistry
• Research Institution: The University of Kitakyushu
• Principal Investigator: 櫻井 和朗 北九州市立大学, 国際環境工学部, 教授 (70343431)
• Co-Investigator(Kenkyū-buntansha): 秋葉 勇 北九州市立大学, 国際環境工学部, 助教授 (80282797)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,800,000 (Direct Cost: ¥3,800,000); Fiscal Year 2006: ¥400,000 (Direct Cost: ¥400,000); Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: β1,3グルカン / カーボンナノナノチューブ / 核酸塩基 / 包摂 / 自己組織化 / 有機ゲル化剤 / ANS / X線小角散乱

Research Abstract

β1,3グルカンが作る2重螺旋には、ピッチ1.8nmの61螺旋の溝ができる。この溝の大きさは核酸塩基を取り込むのに最適の大きさがあり、溝の奥には水素結合サイトが存在することが分かった。事実、コンピューター上で、核酸塩基をその溝に入れてみると極めて容易に包摂される。従って、核酸塩基の親水と疎水性のバランスを化学的に調整さえすれば、GMP、GDPが再構成した多糖の3重螺旋内に内包されると考える。しかし、現在、かなり苦戦をしている。そこで、今年度は、アプローチを変えて、最も疎水性の高いと考えられる、カーボンナノナノチューブの内包に挑戦した。β1,3グルカンがカーボンナノチューブ、特に、単層ナノチューブの優れた可溶化剤になることを見出した。興味深いことに、グルカンがナノチューブ表面をらせん状に被覆し、グルカン特有の周期構造をナノチューブ表面でも再生していることがAFMによる観察の結果から明らかとなった。カーボンナノチューブ表面に規則的なパターンを形成することはナノチューブへの機能付与を考える上でも極めて重要であることは言うまでもない。今後、本系がナノチューブの機能化とバイオテクノロジーへの展開において、重要な役割を果たすものと期待される

Research Products

• [Journal Article] Complex Made from N,N-Bis(carboxymethyl) Glutamate Tetrasodium Salt and Sodium Oleate that Forms a Highly Ordered Lamella in Gel Phase (2007)
  • Author(s): Jeong, Y., Uezu.K., Sakurai, S., Masunaga, H., Inoue, K., Sasaki, S., Shimada, N., Takeda, Y., Kaneko, K., Sakurai, K.
  • Journal Title: Bull. Chem. Soc. Jpn. 80 Pages: 410-410
• [Journal Article] Molecular Dynamics Simulation for b-1,3-D-glucan in Aqueous Solutions : Attachment of One Side Chain Induces an Entire Structural Change (2006)
  • Author(s): Kentaro Miyoshi, Kazuya Uezu, Kazuo Sakurai, Seiji Shinkai
  • Journal Title: Fiber 62 Pages: 251-251
• [Journal Article] Polysaccharide/DNA Supermolecule : Triple Helix Consisting f One olynucleotide and Two Polysaccharide Chains and its Application to Oligo-DNA Delivery Interface : Polynucleotide, Polysaccharide, Molecular Recognition"f One Side Chain Induces an Entire Structural Change (2006)
  • Author(s): Jusaku Minari, Ryouji Karinaga, Seiji Shinkai, Kazuo Sakurai
  • Journal Title: BOTTOM-UP NANOFABRICATION Supramolecules, Self-Assemblies, and Organized Films, chapter 85 Pages: 1-1
• [Journal Article] CpG DNA/zymosan complex to enhance cytokine secretion owing to the cocktail effect. (2006)
  • Author(s): Anada, Takahisa, Okada, Naoko, Minari, Jusaku, Karinaga, Ryouji, Mizu, Masami, Koumoto, Kazuya, Shinkai, Seiji, Sakurai, Kazuo.
  • Journal Title: Bioorganic & Medicinal Chemistry Letters 16(5) Pages: 1301-1304
• [Journal Article] Click chemistry on curdlan : A regioselective and quantitative approach to develop artificial b-1,3-Glucans with various functional appendages. (2006)
  • Author(s): Hasegawa, Teruaki, Umeda, Mariko, Numata, Munenori, Fujisawa, Tomohisa, Haraguchi, Shuichi, Sakurai, Kazuo, Shinkai, Seiji.
  • Journal Title: Chemistry Letters 35(1) Pages: 82-83
• [Journal Article] Galactose-PEG dual conjugation of b-(1,3)-D-glucan schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake. (2006)
  • Author(s): Karinaga, Ryouji, Anada, Takahisa, Minari, Jusaku, Mizu, Masami, Koumoto, Kazuya, Fukuda, Junji, Nakazawa, Kohji, Hasegawa, Teruaki, Numata, Munenori, Shinkai, Seiji, Sakurai, Kazuo.
  • Journal Title: Biomaterials 27(8) Pages: 1626-1635
• [Journal Article] Transition from a Normal to Inverted Cylinder for an Amidine-Bearing Lipid/pDNA Complex and Its Excellent Transfection. (2005)
  • Author(s): Koiwai, Kazunori, Tokuhisa, Kenji, Karinaga, Ryouji, Kudo, Yasuya, Kusuki, Shota, Takeda, Yoichi, Sakurai, Kazuo.
  • Journal Title: Bioconjugate Chemistry 16(6) Pages: 1349-1351
• [Journal Article] Linear double-stranded DNA that mimics an infective tail of virus genome to enhance transfection. (2005)
  • Author(s): Anada, Takahisa, Karinaga, Ryouji, Koumoto, Kazuya, Mizu, Masami, Nagasaki, Takeshi, Kato, Yoshio, Taira, Kazunari, Shinkai, Seiji, Sakurai, Kazuo.
  • Journal Title: Journal of Controlled Release 108(2-3) Pages: 529-539
• [Journal Article] Cholesterol-appended b-(1,3)-D-glucan schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake. (2005)
  • Author(s): Koumoto, Kazuya, Mizu, Masami, Anada, Takahisa, Nagasaki, Takeshi, Shinkai, Seiji, Sakurai, Kazuo.
  • Journal Title: Bulletin of the Chemical Society of Japan 78(10) Pages: 1821-1830
• [Journal Article] Proposal of a Hydrogen Bonding Form, to Maintain the Curdlan Triple Helix (2004)
  • Author(s): K.Miyoshi, K.Uezu, K.Sakurai, S.Shinkai
  • Journal Title: Chemistry &Biodiversity 1(6) Pages: 916-924
• [Journal Article] β-1,3-Glucan (schizophyllan) can act as a one-dimensional host for creation of novel poly(aniline) nano-fiber structures (2004)
  • Author(s): M.Numata, T.Hasegawa, T.Fujisawa, K.Sakurai, S.Shinkai
  • Journal Title: Org.Lett. 6(24) Pages: 4447-4450
• [Journal Article] PEG-appended β-(1→3)-D-glucan schizophyllan for antisense-oligonucleotides delivery to induce endosomal escape.
  • Author(s): R.Karinaga, K.Koumoto, M.Mizu, T.Anada, S.Shinkai, K.Sakurai
  • Journal Title: Biomaterials (in press)
• [Book] Non-viral Gene Therapy : Gene Design and Derivery (2005)
  • Author(s): K.Sakurai, T.Nagasaki, T.Kawazu, T.Nagasaki, Taku Satoh
  • Publisher: Springer-Verlag