蝶の翅に集積した薬理成分の探索

• Project/Area Number: 16658051
• Research Category: Grant-in-Aid for Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Kyoto University
• Principal Investigator: 西田 律夫 京都大学, 農学研究科, 教授 (30135545)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: チョウ類 / ガ類 / 昆虫 / 鱗翅目 / 薬理成分 / 生理活性物質 / 体内蓄積 / 防御物質

Research Abstract

昼行性の鱗翅目昆虫の中には食草に含まれる特異的な二次代謝物を体内に集積することによって身を守っているものが多く知られている.とくに鱗翅目成虫の翅に集積される化合物群に注目し,それらの蓄積過程について,植物側における二次代謝物質動態ならびに昆虫側における化学感覚受容,取込過程,集積・代謝効率などについて調べた.
1)ウマノスズクサを食草とするジャコウアゲハ族各種およびギフチョウ族において,食草由来有毒成分ァリストロキア酸をはじめとするアポルフィン系アルカロイドの虫体含有量を調べた.トリバネアゲハの肉角防御分泌液よりアリストロキア酸ギフチョウの蛹からアリストラクタム類を検出した.しかしいずれも翅への蓄積は認められなかった.
2)ウマノスズクサの培養細胞を作成し,脱分化したカルスから根および葉茎に再分化させた組織におけるアリストロンやアルカロイドのマグノフロリンなどの特異成分の含有量をHPLCなどを駆使して調べた.これら成分の生合成経路について考察した.
3)アゲハチョウの一種ホソオチョウにおける寄主認識に関わる食草成分について追究した.その結果,幼虫の摂食刺激物質として,アリストロキア酸,セクォイトール,モノガラクトシル・ジリノレノイルグリセロールおよび糖類の協力作用を電気生理実験によって明らかにした.とくに,幼虫の口器styloconic sensillaにおける二次代謝物に対する刺激応答シグナルの精密な分析手法を確立した.これにより,虫体蓄積される物質に対する鋭敏な化学受容応答性が適応的に機能していることが判明した.

Research Products

• [Journal Article] Zingerone in floral synomone of Bulbophyllum baileyi (Orchidaceae) attracts Bactrocera fruit fly during pollination. (2007)
  • Author(s): Tan, K.H., Nishida, R.
  • Journal Title: Biochem.Syst.Ecol. 35(in press)
• [Journal Article] Pharmacophagy of methyl eugenol by males enhances sexual selection of Bactrocera carambolae (Diptera : Tephritidae) (2007)
  • Author(s): Wee, Suk Ling, Tan, K.H., Nishida, R.
  • Journal Title: J.Chem.Ecol. 33(in press)
• [Journal Article] Chemical basis of feeding behavior of a bean aphid, Megoura crassicauda, controlled by primary and secondary substances in the host Vicia angustifolia (2006)
  • Author(s): Takemura, M., Kuwahara, Y., Nishida, R.
  • Journal Title: Entomol.Exp.Appl. 121 Pages: 51-57
• [Journal Article] Floral phenylpropanoid cocktail and architecture of Bulbophyllum vinaceum orchid in attracting fruit flies for pollination (2006)
  • Author(s): Tan, K.H., Tan, L.T., Nishida, R.
  • Journal Title: J.Chem.Ecol. 32 Pages: 2429-2441
• [Journal Article] A hemiterpene glucoside as a probing deterrent of the bean aphid, Megoura crassicauda, from a non-host vetch, Vicia hirsuta (2006)
  • Author(s): Ohta, N., Mori, M., Kuwahara, Y., Nishida, R.
  • Journal Title: Phytochemistry 67 Pages: 84-588
• [Journal Article] Synthesis of (+)(S)-robinal together with its antipode, a cyclic monoterpene functioning as the sex pheromone of Rhizoglyphus setosus and its distribution among Astigmata (2006)
  • Author(s): Shimizu, N., Mori, N., Nishida, R., Kuwahara, Y.et al.
  • Journal Title: J.Pestic.Sci. 31 Pages: 311-315
• [Journal Article] Synomone or kairomone? - Bulbophylum apertum flower releases raspberry ketone to attract Bactrocera fruit flies (2005)
  • Author(s): Tan, K.H., Nishida, R.
  • Journal Title: J. Chem. Ecol. 31 Pages: 509-519
• [Journal Article] Identification of astigmatid mites using the second internal transcribed spacer(ITS2)region and its application (2005)
  • Author(s): Noge, K., Mori, N., Tanaka, C., Nishida, R.et al.
  • Journal Title: Exp. Appl. Acar. 35 Pages: 29-46
• [Journal Article] In vitro biosynthesis of volicitin in Spodoptera litura (2005)
  • Author(s): Yoshinaga, N., Morigaki, N., Matsuda, F., Nishida, R., Mori, N.
  • Journal Title: Insect Biochem. Mol. Biol. 36 Pages: 175-184
• [Journal Article] S-isorobinal as the female sex pheromone from an alarm pheromone-emitting mite Rhizoglyphus setosus (2005)
  • Author(s): Mizoguchi, A.., Mori, N., Nishida, R., Kuwahara, Y.et al.
  • Journal Title: Exp. Appl. Acarol. 35 Pages: 29-46
• [Journal Article] Scheloribatid mites as the source of pumiliotoxin in dendrobatid (2005)
  • Author(s): Takada, W., Sakata, Mori, N., Nishida, R., Kuwahara, Y.et al.
  • Journal Title: J. Chem. Ecol. 14 Pages: 2403-2415
• [Journal Article] Pharmacophagous feeding stimulant activity of neo-clerodane diterpenoids for the turnip sawfly, Athalia rosae ruficornis (2004)
  • Author(s): Nishida, R., Kawai, K., Amano, T., Kuwahara, Y.
  • Journal Title: Biochem.Syst.Ecol. 32 Pages: 15-25
• [Journal Article] Phenylpropanoids in the fragrance of the fruit fly orchid, Bulbophyllum cheiri, and their relationship to the pollinator, Bactrocera papayae (2004)
  • Author(s): Nishida, R., Tan, K.H., Wee, S.L., Hee, A.K.W., Toong, Y.
  • Journal Title: Biochem.Syst.Ecol. 32 Pages: 245-252
• [Journal Article] Hydroxybenzoic acid derivatives in a non-host rutaceous plant, Orixa japonica, deter both oviposition and larval feeding in a Rutaceae-swallowtail butterfly, Papilio xuthus (2004)
  • Author(s): Ono, H., Kuwahara, Y., Nishida, R.
  • Journal Title: J.Chem.Ecol. 30 Pages: 287-301
• [Journal Article] Larval and adult feeding on methyl eugenol and the mating success of male oriental fruit flies, Bactrocera dorsali (2004)
  • Author(s): Shelly, T.E., Nishida, R
  • Journal Title: Entomol.Exp.Appl. 112 Pages: 155-158
• [Journal Article] Neral biosynthesis via the mavalonate pathway, by D-glucose-1-^<13>C Feeding in Carpoglyphus lactis and ^<13>C incorporation into other opisthonotal gland exudate (2004)
  • Author(s): Morita A., Mori, N., Nishida, R., Hirai, N., Kuwahara, Y.
  • Journal Title: J.Pestic.Sei. 29 Pages: 27-32
• [Journal Article] Synomone or kairomone? - Bulbophyllum apertum flower releases raspberry ketone to attract Bactrocera fruit flies (2004)
  • Author(s): Tan, K.H., Nishida, R
  • Journal Title: J.Chem.Ecol. 31 Pages: 509-519
• [Book] 植物の化学防衛:「プラントミメティックッス〜植物に学ぶ〜」 (2006)
  • Author(s): 西田律夫(分担執筆)
  • Publisher: NTS,東京