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マウス着床後発生における胚ー子宮相互作用の研究

Research Project

Project/Area Number 16F16089
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Developmental biology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

藤森 俊彦  基礎生物学研究所, 初期発生研究部門, 教授 (80301274)

Co-Investigator(Kenkyū-buntansha) DAY TIMOTHY  基礎生物学研究所, 初期発生研究部門, 外国人特別研究員
Project Period (FY) 2016-04-22 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2017: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords鉄イオン
Outline of Annual Research Achievements

I investigated the morphological changes of both the uterus and the extraembryonic tissues through analysis of 3D rendered images of post-implantation embryos. Proof of principle was determined by making 3D reconstructions of heart tissues from serially sectioned 9.5 dpc embryos, scanned at high resolution, aligned by unique and precise automated software, and then delineated from surrounding tissues by manual tracing throughout 241 serial sections using the free open-source software FIJI/TrakEM2. Gene expression analysis with reverse-transcription quantitative PCR of iron regulatory genes expression was performed, and analysis through immunofluorescence of non-pregnant and pregnant stages of 4.5-9.5 days. SLC40a1 protein was upregulated in non-pregnant females is particularly high in the LE and GE of the uterus. Female SLC40a1 transgenic uteri were collected at several stages of the uterine cycle and stained for LacZ. This showed that SLC40a1 is most highly expressed during the estrus cycle. High expression of Lacz staining is exhibited in the luminal epithelium and glandular epithelium with little or no expression in the underlying stromal tissue. Concurrently, analysis of the ovary and oviduct revealed consistent expression of SLC40a1-driven LacZ independent of estrus cycle, suggesting a differential expression mechanism of ovarian SlC40a1 vs uterine SLC40a1. DAB staining of both non-pregnant and pregnant female WT mice exhibited differential expression patterns consistent with promoter driven Lacz staining and immunofluorescence.

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (3 results)

All 2016 Other

All Presentation (1 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Presentation] High resolution imaging and 3D reconstruction of developing mouse embryos in the uterus.2016

    • Author(s)
      Timothy Day
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 基礎生物学研究所・初期発生研究部門

    • URL

      http://www.nibb.ac.jp/embryo/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 基礎生物学研究所・初期発生研究部門

    • URL

      http://www.nibb.ac.jp/embryo/index.html

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-05-17   Modified: 2024-03-26  

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