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CRISPR/Cas9封入高分子集合体の構築と生体内デリバリーへの挑戦

Research Project

Project/Area Number 16F16355
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionThe University of Tokyo

Principal Investigator

宮田 完二郎  東京大学, 大学院工学系研究科(工学部), 准教授 (50436523)

Co-Investigator(Kenkyū-buntansha) MIN HYUN SU  東京大学, 工学(系)研究科(研究院), 外国人特別研究員
Project Period (FY) 2016-10-07 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2018: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2017: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2016: ¥600,000 (Direct Cost: ¥600,000)
KeywordsCas9 / messenger RNA delivery / polyplex / polymeric micelle / Muscular dystrophy / amphiphilic polymer / 薬物送達システム / CRISPR
Outline of Annual Research Achievements

Cas9 activities of various cationic polymers were evaluated by luciferase-based Cas9 activity system. This assay consists of three different plasmids - sgRNA-expressing plasmid, Stop codon-contained luciferase-expressing plasmid, and luciferase donor DNA plasmid, which were kindly donated by Professor Moritoshi Sato (UTokyo). A cationic polymer (PAsp(OCT/DET)) was complexed with Cas9 messenger RNA and then the resulting polyplexes were transfected into human cervical cancer (HeLa) cells. The Cas9 activities were measured by a luminescence microplate reader. The cationic polymer elicited the similar luminescence intensity compared to commercially available transfection agent, lipofectamine 3000. This result indicates that the new cationic polymer is promising for in vitro Cas9 mRNA transfection.

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (16 results)

All 2019 2018 2017 2016 Other

All Presentation (13 results) (of which Int'l Joint Research: 4 results,  Invited: 10 results) Book (1 results) Remarks (2 results)

  • [Presentation] リガンド搭載高分子ミセルの構築と核酸医薬デリバリーへの展開2019

    • Author(s)
      宮田完二郎, ミン ヒョンス, イ ウ, 内藤瑞, キム ヒョンジン, 片岡一則
    • Organizer
      第67回高分子討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 抗体リガンド搭載高分子ミセルの構築とがんへの核酸医薬デリバリー2019

    • Author(s)
      宮田完二郎, ミン ヒョンス, キム ヒョンジン, 内藤瑞, 松村保広, 片岡一則
    • Organizer
      日本核酸医薬学会第4回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Smart oligonucleotide delivery by polymer nanotechnology2019

    • Author(s)
      K. Miyata
    • Organizer
      The 35th International Conference of Photopolymer Science and Technology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of thiol-crosslinked micelle for systemic delivery of antisense oligonucleotide to treat brain diseases2019

    • Author(s)
      H. -J. Kim, H. -S. Min, K. Katsushima, Y. Kondo, K. Miyata, K. Kataoka
    • Organizer
      第67回高分子年次大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 核酸医薬デリバリーのための高分子材料設計2018

    • Author(s)
      宮田完二郎
    • Organizer
      第13回理研「デジタル×バイオものづくり」シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Polymer-based oligonucleotide delivery ~challenges and solutions~2017

    • Author(s)
      Kanjiro Miyata
    • Organizer
      11th Annual Symposium on Nanobiotechnology 2017
    • Place of Presentation
      Kawasaki, Japan
    • Year and Date
      2017-02-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 核酸医薬のドラッグデリバリーシステム2017

    • Author(s)
      宮田完二郎
    • Organizer
      第36回日本認知症学会学術集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Small polymeric nanocarriers for smart oligonucleotide delivery2017

    • Author(s)
      K. Miyata
    • Organizer
      Beijing Symposium 2017: Polymeric Biomaterials
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高分子・無機材料を用いたナノ構造体の創製と核酸医薬デリバリーへの応用2017

    • Author(s)
      宮田完二郎
    • Organizer
      日本核酸医薬学会第3回年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 高分子材料を基盤とする核酸医薬デリバリー2017

    • Author(s)
      宮田完二郎
    • Organizer
      CBI学会 第384回研究講演会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ナノマシンによるがん治療の最前線2017

    • Author(s)
      宮田完二郎
    • Organizer
      先端ナノデバイス・材料テクノロジー第151委員会
    • Place of Presentation
      川崎 川崎生命科学・環境研究センター
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Development of smart polymeric nanocarriers for targeted siRNA delivery2016

    • Author(s)
      Kanjiro Miyata
    • Organizer
      EPFL/UTokyo Joint Symposium 2016, Lausanne
    • Place of Presentation
      Lausanne, Switzerland
    • Year and Date
      2016-12-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高分子ナノキャリアを用いた核酸医薬デリバリー2016

    • Author(s)
      宮田完二郎
    • Organizer
      第1回RNA創薬セミナー
    • Place of Presentation
      大阪 大阪医科大学
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Book] DDS先端技術の製剤への応用開発2017

    • Author(s)
      宮田完二郎、他
    • Total Pages
      492
    • Publisher
      技術情報協会
    • ISBN
      9784861046636
    • Related Report
      2017 Annual Research Report
  • [Remarks] 宮田研ホームページ

    • URL

      http://www.bmm.t.u-tokyo.ac.jp/index.html

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Remarks] 宮田研究室ホームページ

    • URL

      http://www.bmm.t.u-tokyo.ac.jp/index.html

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-10-11   Modified: 2024-03-26  

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