| Project/Area Number |
16F16355
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | The University of Tokyo |
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Principal Investigator |
宮田 完二郎 東京大学, 大学院工学系研究科(工学部), 准教授 (50436523)
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| Co-Investigator(Kenkyū-buntansha) |
MIN HYUN SU 東京大学, 工学(系)研究科(研究院), 外国人特別研究員
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| Project Period (FY) |
2016-10-07 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2018: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2017: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2016: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | Cas9 / messenger RNA delivery / polyplex / polymeric micelle / Muscular dystrophy / amphiphilic polymer / 薬物送達システム / CRISPR |
|---|
| Outline of Annual Research Achievements |
Cas9 activities of various cationic polymers were evaluated by luciferase-based Cas9 activity system. This assay consists of three different plasmids - sgRNA-expressing plasmid, Stop codon-contained luciferase-expressing plasmid, and luciferase donor DNA plasmid, which were kindly donated by Professor Moritoshi Sato (UTokyo). A cationic polymer (PAsp(OCT/DET)) was complexed with Cas9 messenger RNA and then the resulting polyplexes were transfected into human cervical cancer (HeLa) cells. The Cas9 activities were measured by a luminescence microplate reader. The cationic polymer elicited the similar luminescence intensity compared to commercially available transfection agent, lipofectamine 3000. This result indicates that the new cationic polymer is promising for in vitro Cas9 mRNA transfection.
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| Research Progress Status |
平成30年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
平成30年度が最終年度であるため、記入しない。
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