インフルエンザウイルスのゲノムパッケージング機構の解明
| Project/Area Number |
16F16416
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Research Field |
Virology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
河岡 義裕 東京大学, 医科学研究所, 教授 (70135838)
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| Co-Investigator(Kenkyū-buntansha) |
WANG I-HSUAN 東京大学, 医科学研究所, 外国人特別研究員
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| Project Period (FY) |
2016-11-07 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2018: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2017: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2016: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Influenza virus / Viral genome packaging / Virus budding / Virus-host interaction / Nanoscopy / Single-molecule FISH |
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| Outline of Annual Research Achievements |
In this project, we employed a nanoscopic approach that combines a cell unroofing method, freeze-etching electron microscopy and live atomic force microscopy to resolve the mechanism behind virion formation and the involvement of cellular machineries in influenza A virus (IAV) replication. By using immuno-labeling to identify various cellular proteins found in the previous experiments, we verified few key host factor(s) and how they interact with viral components to facilitate the viral genome assembly and budding (a manuscript in preparation).I also worked on the another project which was aimed at developing intravital imaging assays to study disease progression in the lung of influenza virus-infected mice. We revealed an early and transient recruitment of pulmonary intravascular neutrophils induced by influenza virus infection (Ueki, Wang et al., PNAS, 2018).
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| Research Progress Status |
平成30年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
平成30年度が最終年度であるため、記入しない。
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Report
(3 results)
Research Products
(5 results)
