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インフルエンザウイルスのゲノムパッケージング機構の解明

Research Project

Project/Area Number 16F16416
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Virology
Research InstitutionThe University of Tokyo

Principal Investigator

河岡 義裕  東京大学, 医科学研究所, 教授 (70135838)

Co-Investigator(Kenkyū-buntansha) WANG I-HSUAN  東京大学, 医科学研究所, 外国人特別研究員
Project Period (FY) 2016-11-07 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2018: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2017: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2016: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsInfluenza virus / Viral genome packaging / Virus budding / Virus-host interaction / Nanoscopy / Single-molecule FISH
Outline of Annual Research Achievements

In this project, we employed a nanoscopic approach that combines a cell unroofing method, freeze-etching electron microscopy and live atomic force microscopy to resolve the mechanism behind virion formation and the involvement of cellular machineries in influenza A virus (IAV) replication. By using immuno-labeling to identify various cellular proteins found in the previous experiments, we verified few key host factor(s) and how they interact with viral components to facilitate the viral genome assembly and budding (a manuscript in preparation).I also worked on the another project which was aimed at developing intravital imaging assays to study disease progression in the lung of influenza virus-infected mice. We revealed an early and transient recruitment of pulmonary intravascular neutrophils induced by influenza virus infection (Ueki, Wang et al., PNAS, 2018).

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (5 results)

All 2018 2017

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] In vivo imaging of the pathophysiological changes and neutrophil dynamics in influenza virus-infected mouse lungs.2018

    • Author(s)
      Ueki H, Wang IH, Fukuyama S, Katsura H, da Silva Lopes TJ, Neumann G, Kawaoka Y
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 115 Issue: 28

    • DOI

      10.1073/pnas.1806265115

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Imaging, Tracking and Computational Analyses of Virus Entry and Egress with the Cytoskeleton2018

    • Author(s)
      Wang I-Hsuan、Burckhardt Christoph、Yakimovich Artur、Greber Urs
    • Journal Title

      Viruses

      Volume: 10 Issue: 4 Pages: 166-166

    • DOI

      10.3390/v10040166

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Understanding influenza A virus budding by direct visualization of viral ribonucleoproteins at the plasma membrane.2018

    • Author(s)
      Wang IH, Usukura J, Kawaoka Y.
    • Organizer
      The 66th Annual Meeting of the Japanese Society for Virology
    • Related Report
      2018 Annual Research Report
  • [Presentation] Understanding influenza A virus budding by direct visualization of viral ribonucleoproteins at the plasma membrane.2018

    • Author(s)
      Wang I-Hsuan
    • Organizer
      7th Negative Strand Virus-Japan
    • Related Report
      2017 Annual Research Report
  • [Presentation] Identification of host factors involved in the genome packaging of influenza A virus by using quantitative microscopy2017

    • Author(s)
      I-Hsuan Jessica Wang
    • Organizer
      6th Negative Strand Virus-Japan Symposium
    • Place of Presentation
      沖縄
    • Year and Date
      2017-01-16
    • Related Report
      2016 Annual Research Report

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Published: 2016-11-08   Modified: 2024-03-26  

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