| Project/Area Number |
16F16753
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Research Field |
General internal medicine (including psychosomatic medicine)
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| Research Institution | Tohoku University |
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Principal Investigator |
福土 審 東北大学, 医学系研究科, 教授 (80199249)
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| Co-Investigator(Kenkyū-buntansha) |
ZHANG YANLI 東北大学, 医学(系)研究科(研究院), 外国人特別研究員
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| Project Period (FY) |
2016-11-07 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2018: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2017: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2016: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | 内臓知覚 / 内臓知覚過敏 / CRH / CRF / CRH受容体1 / 過敏性腸症候群 / 腹壁筋電図 / 不安行動 / ストレス / 大腸運動 / 不安 / 動物実験 / 5-hydroxytryptamine / 内臓痛 / 脳腸相関 / 神経伝達物質 / 動物モデル |
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| Outline of Annual Research Achievements |
Aim: Corticotropin-releasing hormone (CRH) and its receptors play important roles in colonic response to stress. We tested the hypothesis that peripheral CRH receptor 1 (CRH-R1) activation induces colonic visceromotor dysfunction as well as anxiety behavio. Methods: Male Wistar rats were divided into four groups. Either vehicle or CRH-R1 antagonist (CP-154,526) was injected subcutaneously 30 min before intraperitoneal injection of CRH-R1 agonist cortagine or vehicle. Colorectal distention (CRD) was performed two times over 20 minutes. The electromyogram (EMG) was recorded to measure the visceromotor responses (VMR) to CRD. Anxiety-related behavior was measured for 5 minutes during exposure to an elevated-plus maze (EPM). Results: Compared with vehicle, cortagine significantly induced increase in fecal pellets number (P < 0.05). CP-154,526 abolished the cortagine-induced fecal pellets output (P < 0.05). The amplitude of EMG has significant differences among four groups (P < 0.01). In the rats treated with cortagine, EMG amplitude was significantly higher than that control and CP group (P < 0.05). CP-154,526 has significantly decreased amplitude, compared with cortagine group (P < 0.05). The total travel distance in the EPM has significantly differences among four groups (P = 0.01). Conclusions: Our results suggest that peripheral cortagine injection can stimulate defecation and induce visceral hypersensitivity in rats, which were abolished by CRH-R1 antagonist. Peripheral cortagine may induce anxiety-related behaviour while CRH-R1 antagonist reduces the anxiety behavior.
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| Research Progress Status |
平成30年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
平成30年度が最終年度であるため、記入しない。
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