| Budget Amount *help |
¥41,340,000 (Direct Cost: ¥31,800,000、Indirect Cost: ¥9,540,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2017: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2016: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
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| Outline of Final Research Achievements |
We demonstrated the relevance of three PCNA interacting peptide motifs of Pol eta for the regulation of translesion DNA synthesis in human cells. We also identified a region, which is different from the previous report, of Pol eta to interact with RAD18. We reported the molecular mechanisms of mono- and poly-ubiquitination reactions of PCNA by usisng reconstituted in vitro systems. Then, molecular mechanism of the deubiquitination reaction by USP7 is also demonstrated. Finally, we constituted the system to analyse the DNA damage tolerance pathway regulated by the multiple modifications of a PCNA homo trimer.
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