Higher visual areas responsible for shape recognition in mice

• Project/Area Number: 16H01892
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Brain biometrics
• Research Institution: Niigata University
• Principal Investigator: Shibuki Katsuei 新潟大学, 脳研究所, 非常勤講師 (40146163)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥44,200,000 (Direct Cost: ¥34,000,000、Indirect Cost: ¥10,200,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2018: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2017: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2016: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
• Keywords: 高次視覚野 / 図形認知 / マウス / 皮質盲 / 視覚的意識 / 聴覚野 / 視覚野 / 連想記憶 / フラビン蛋白蛍光イメージング / 短期記憶 / 遅延見本合わせ / イメージング / 一次視覚野 / 上丘 / 長期増強 / 自動学習実験

Outline of Final Research Achievements

We investigated the responses in the higher visual cortex of conscious mice. The posterior-ventral cortical areas adjacent to the auditory cortex responded to moving object stimuli. Visual object recognition can be performed in wide cortical regions including these areas. Analysis of associative auditory responses to visual stimuli revealed that the associative responses were observed in diffuse auditory areas including the anterior auditory field. The formation of associative memory required stimulus complexity, which may be essential for characterizing stimuli. Visual object recognition is related to visual consciousness. Damages to the primary visual cortex (V1) impair visual consciousness. We investigated a male patient with the damaged V1. Repeated presentation of dynamic visual stimuli to him resulted in long-term potentiation in the probability of detection of visual stimuli, and after this potentiation, he could read hiragana letters presented in the impaired visual field.

Academic Significance and Societal Importance of the Research Achievements

我々は覚醒マウスの高次視覚野応答を解析し、高次視覚野が聴覚野の腹側まで広がっているという新知見を得た。またその部位の性質は一次視覚野の性質と大きく異なることが判った。これらの成果は、図形認知機能の分子機構解明に有用である。図形認知機能は、視覚的意識にかかわるが、視覚的意識を調べるためには、言語による報告が可能な人間を解析対象とする必要がある。我々は一次視覚野が脳梗塞によって障害された被検者の文字を読む図形認知機能について解析し、これらが動的視覚刺激の繰り返し提示によって回復することを示した。本研究の成果は、皮質盲の診断や治療といった臨床医学的観点からも有用であると考えられる。

Research Products

• [Journal Article] Rapid Recovery From Cortical Blindness Caused by an Old Cerebral Infarction (2020)
  • Author(s): Shibuki Katsuei、Wakui Ichiro、Fujimura Takeo、Tomikawa Masaru、Hasegawa Shin
  • Journal Title: Frontiers in Neurology Volume: 11 Pages: 1-7
  • DOI: 10.3389/fneur.2020.00069
  • Peer Reviewed / Open Access
• [Journal Article] Reciprocal connectivity between secondary auditory cortical field and amygdala in mice. (2019)
  • Author(s): Tsukano H, Hou X, Horie M, Kitaura H, Nishio N, Hishida R, Takahashi K, Kakita A, Takebayashi H, Sugiyama S, Shibuki K
  • Journal Title: Sci Rep. Volume: 9 Issue: 1 Pages: 19610-19610
  • DOI: 10.1038/s41598-019-56092-9
  • Peer Reviewed / Open Access
• [Journal Article] Associative responses to visual shape stimuli in the mouse auditory cortex. (2019)
  • Author(s): Ogi M, Yamagishi T, Tsukano H, Nishio N, Hishida R, Takahashi K, Horii A, Shibuki K
  • Journal Title: PLoS One Volume: 14 Issue: 10 Pages: 1-13
  • DOI: 10.1371/journal.pone.0223242
  • Peer Reviewed / Open Access
• [Journal Article] Feedback inhibition derived from the posterior parietal cortex regulates the neural properties of the mouse visual cortex. (2019)
  • Author(s): Hishida R, Horie M, Tsukano H, Tohmi M, Yoshitake K, Meguro R, Takebayashi H, Yanagawa Y, Shibuki K
  • Journal Title: Eur J Neurosci Volume: 50 Issue: 6 Pages: 2970-2987
  • DOI: 10.1111/ejn.14424
  • Peer Reviewed
• [Journal Article] Acute spatial spread of NO-mediated potentiation during hindpaw ischaemia in mice. (2019)
  • Author(s): Onishi T, Watanabe T, Sasaki M, Kamiya Y, Horie M, Tsukano H, Hishida R, Kohno T, Takebayashi H, Baba H, Shibuki K
  • Journal Title: J Physiol. Volume: 597 Issue: 13 Pages: 3441-3455
  • DOI: 10.1113/jp277615
  • Peer Reviewed / Open Access
• [Journal Article] Direct Relay Pathways from Lemniscal Auditory Thalamus to Secondary Auditory Field in Mice. (2018)
  • Author(s): Ohga S, Tsukano H, Horie M, Terashima H, Nishio N, Kubota Y, Takahashi K, Hishida R, Takebayashi H, Shibuki K.
  • Journal Title: Cereb Cortex Volume: 28 Issue: 12 Pages: 4424-4439
  • DOI: 10.1093/cercor/bhy234
  • Peer Reviewed / Open Access
• [Journal Article] Higher visual responses in the temporal cortex of mice (2018)
  • Author(s): Nishio Nana、Tsukano Hiroaki、Hishida Ryuichi、Abe Manabu、Nakai Junichi、Kawamura Meiko、Aiba Atsushi、Sakimura Kenji、Shibuki Katsuei
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 11136-11136
  • DOI: 10.1038/s41598-018-29530-3
  • Peer Reviewed / Open Access
• [Journal Article] Molecular diversity of clustered protocadherin-α required for sensory integration and short-term memory in mice. (2018)
  • Author(s): Yamagishi T, Yoshitake K, Kamatani D, Watanabe K, Tsukano H, Hishida R, Takahashi K, Takahashi S, Horii A, Yagi T, Shibuki K.
  • Journal Title: Sci Rep. Volume: 8 Issue: 1 Pages: 9616-9616
  • DOI: 10.1038/s41598-018-28034-4
  • Peer Reviewed / Open Access
• [Journal Article] Tomographic optical imaging of cortical responses after crossing nerve transfer in mice (2018)
  • Author(s): Maniwa K, Yamashita H, Tsukano H, Hishida R, Endo N, Shibata M, Shibuki K
  • Journal Title: PLoS One Volume: 13 Issue: 2 Pages: e0193017-e0193017
  • DOI: 10.1371/journal.pone.0193017
  • Peer Reviewed / Open Access
• [Journal Article] Chondroitin Sulfate Is Required for Onset and Offset of Critical Period Plasticity in Visual Cortex (2017)
  • Author(s): Hou X, Yoshioka N, Tsukano H, Sakai A, Miyata S, Watanabe Y, Yanagawa Y, Sakimura K, Takeuchi K, Kitagawa H, Hensch TK, Shibuki K, Igarashi M, Sugiyama S
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 12646-12646
  • DOI: 10.1038/s41598-017-04007-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reconsidering Tonotopic Maps in the Auditory Cortex and Lemniscal Auditory Thalamus in Mice. (2017)
  • Author(s): 1.Tsukano H, Horie M, Ohga S, Takahashi K, Kubota Y, Hishida R, Takebayashi H, Shibuki K
  • Journal Title: Front Neural Circuits Volume: 11 Pages: 14-14
  • DOI: 10.3389/fncir.2017.00014
  • Peer Reviewed / Open Access
• [Journal Article] Independent tonotopy and thalamocortical projection patterns in two adjacent parts of the classical primary auditory cortex in mice. (2017)
  • Author(s): 2.Tsukano H, Horie M, Takahashi K, Hishida R, Takebayashi H, Shibuki K
  • Journal Title: Neurosci Lett Volume: 637 Pages: 26-30
  • DOI: 10.1016/j.neulet.2016.11.062
  • Peer Reviewed / Open Access
• [Journal Article] Auditory cortical field coding long-lasting tonal offsets in mice. (2016)
  • Author(s): 3.Baba H, Tsukano H, Hishida R, Takahashi K, Horii A, Takahashi S, Shibuki K
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 34421-34421
  • DOI: 10.1038/srep34421
  • Peer Reviewed / Open Access
• [Presentation] Feasibility of forward genetic analysis of consciousness using mouse models (2017)
  • Author(s): Shibuki K
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: アクトシティ浜松(静岡県浜松市)
  • Year and Date: 2017-03-28
  • Invited
• [Presentation] Tonotopic properties in the secondary auditory field are derived from structural organization of the ventral medial geniculate body and thalamocortical projections in mice (2017)
  • Author(s): 塚野浩明、大賀慎平、堀江正男、寺島裕貴、竹林浩秀、澁木克栄
  • Organizer: 第40回日本神経科学大会
• [Presentation] Plastic changes of visual responses in the primary visual cortex are induced selectively by inhibitory projection from posterior parietal cortex in mice (2017)
  • Author(s): 菱田竜一、堀江正男、塚野浩明、任海学、澁木克栄
  • Organizer: 第40回日本神経科学大会
• [Presentation] Complexity of sound stimuli required for sound-shape associative responses in the mouse auditory cortex (2017)
  • Author(s): 小木学、山岸達矢、塚野浩明、西尾奈々、菱田竜一、堀井新、八木健、澁木克栄
  • Organizer: 第40回日本神経科学大会
• [Presentation] Optical imaging of temporal cortical areas involved in audiovisual integration in awake mice (2017)
  • Author(s): 西尾奈々、小木学、山岸達矢、塚野浩明、菱田竜一、八木健、澁木克栄
  • Organizer: 第40回日本神経科学大会
• [Presentation] Analysis of prediction error responses in the posterior parietal cortex of awake mice (2017)
  • Author(s): 吉武講平、塚野浩明、菱田竜一、八木健、澁木克栄
  • Organizer: 第40回日本神経科学大会
• [Presentation] Functional footprints of impaired consciousness in mice with reduced molecular diversity of clustered protocadherin-α (2016)
  • Author(s): Yamagishi T, Kamatani D, Yoshitake K, Tsukano H, Watanabe K, Hishida R, Takahashi K, Takahashi S, Horii A, Yagi T, Shibuki K
  • Organizer: Neuroscience 2016 Annual Meeting
  • Place of Presentation: San Diego(アメリカ合衆国)
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] Molecular approach to neural mechanisms of consciousness using mouse models. (2016)
  • Author(s): Shibuki K
  • Organizer: 本神経科学大会サテライトシンポジウム：The Origin of Consciousness
  • Place of Presentation: 東京工業大学(東京都目黒区)
  • Year and Date: 2016-07-25
  • Invited
• [Presentation] Neural correlates of visual awareness organized by short-term memory in mice (2016)
  • Author(s): Shibuki K
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2016-07-20
  • Invited
• [Patent(Industrial Property Rights)] マウス用学習装置、マウス用学習システム及びマウス用学習プログラム (2018)
  • Inventor(s): 澁木克栄
  • Industrial Property Rights Holder: 新潟大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2018
  • Overseas
• [Patent(Industrial Property Rights)] マウス用学習装置、マウス用学習システム及びマウス用学習プログラム (2017)
  • Inventor(s): 澁木 克栄
  • Industrial Property Rights Holder: 新潟大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-034171
  • Filing Date: 2017-02-24