Near infrared fluorescent imaging of brown adipose tissues with carbon nanotubes
| Project/Area Number |
16H02085
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials chemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Yudasaka Masako 国立研究開発法人産業技術総合研究所, 材料・化学領域, 招聘研究員 (70159226)
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| Co-Investigator(Kenkyū-buntansha) |
片浦 弘道 国立研究開発法人産業技術総合研究所, 材料・化学領域, 首席研究員 (30194757)
佐伯 久美子 国立研究開発法人国立国際医療研究センター, その他部局等, その他 (80322717)
岡松 優子 北海道大学, 獣医学研究院, 准教授 (90527178)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥42,640,000 (Direct Cost: ¥32,800,000、Indirect Cost: ¥9,840,000)
Fiscal Year 2018: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2017: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2016: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
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| Keywords | カーボンナノチューブ / 褐色脂肪組織 / 近赤外蛍光造影剤 / イメージング / 近赤外蛍光造影 / 血管外漏出性亢進 / フラーレン。ナノチューブ・グラフェン / バイオ応用 / ナノチューブ・フラーレン / ナノバイオ / ナノチューブ |
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| Outline of Final Research Achievements |
This study has revealed that near infrared (NIR) fluorescence imaging with single-walled carbon nanotubes (CNTs) can selectively image brown adipose tissue (BAT) and monitor the BAT changes in mice induced by the external stimulation when the surfaces of CNTs are properly coated. Furthermore, it has been shown that the NIR fluorescence microscopy enables the observation of CNT distribution in cellular levels, revealing that the BAT vascular permeability is enhanced under fasting in mice. Gene analysis indicated that protease was upregulated under the fasting, which suggested that basal membranes of capillary/arteriolar walls were degenerated as demonstrated by the disarranged or broken collagen bands in the extracellular matrix.
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| Academic Significance and Societal Importance of the Research Achievements |
脂肪を燃焼させる褐色脂肪組織（BAT）は、肥満や糖尿病などのメタボリックシンドロームのための新規予防・治療法開発の標的組織として近年注目されている。これまでBATに対しては、簡便な可視化手段がないという問題があったが、本研究では、近赤外（NIR）蛍光造影剤として知られている単層カーボンナノチューブ（CNT）がその表面被覆を制御することで優れたBAT選択的な蛍光造影剤になることを明らかにした。また、NIR蛍光顕微鏡を用いたCNT分布の細胞レベルでの観察により、未知なBAT機能を明らかにできた。こうした成果は、CNTを用いたBAT造影がBAT研究の発展に寄与することを示唆している。
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Report
(5 results)
Research Products
(17 results)
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[Journal Article] "Near-Infrared Photoluminescent Carbon Nanotubes for Imaging of Brown Fat2017
Author(s)
Masako Yudasaka, Yohei Yomogida, Minfang Zhang, Takeshi Tanaka, Masako Nakahara, Norihiko Kobayashi, Yuko Okamatsu-Ogura, Ken Machida, Kazuhiko Ishihara, Kumiko Saeki, Hiromichi Kataura
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Journal Title
Scientfic Reports
Volume: 7
Issue: 1
Pages: 44760-44760
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Near-Infrared Photoluminescent Carbon Nanotubes for Imaging of Brown Fat2017
Author(s)
Masako Yudasaka, Yohei Yomogida, Minfang Zhang, Takeshi Tanaka, Masako Nakahara, Norihiko Kobayashi, Yuko Okamatsu-Ogura, Ken Machida, Kazuhiko Ishihara, Kumiko Saeki, Hiromichi Kataura
Organizer
フラーレン・ナノチューブ・グラフェン学会,第53回シンポジウム
Related Report
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[Presentation] Near-Infrared Photoluminescent Carbon Nanotubes for Imaging of Brown Fat2017
Author(s)
Masako Yudasaka, Yohei Yomogida, Minfang Zhang, Takeshi Tanaka, Masako Nakahara, Norihiko Kobayashi, Yuko Okamatsu-Ogura, Ken Machida, Kazuhiko Ishihara, Kumiko Saeki, Hiromichi Kataura
Organizer
第7回ナノカーボンバイオサテライト
Related Report
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[Presentation] Near-Infrared Photoluminescent Carbon Nanotubes for Imaging of Brown Fat2017
Author(s)
Masako Yudasaka, Yohei Yomogida, Minfang Zhang, Takeshi Tanaka, Masako Nakahara, Norihiko Kobayashi, Yuko Okamatsu-Ogura, Ken Machida, Kazuhiko Ishihara, Kumiko Saeki, Hiromichi Kataura
Organizer
11th International Sympoisum of Nanomedicine
Related Report
Int'l Joint Research / Invited
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