| Budget Amount *help |
¥41,860,000 (Direct Cost: ¥32,200,000、Indirect Cost: ¥9,660,000)
Fiscal Year 2018: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2017: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2016: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
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| Outline of Final Research Achievements |
Aiming at artificial cells, biosystems based on the reconstituted gene expression system, PURE system, have been developed. The proteins involved in the cell division system were synthesized in liposomes and liposomes appeared to deform. By expressing ATP synthetase and bacteriorhodopsin in liposome, a light-dependent energy production system was constructed. For the small subunit of ribosomes, an assembly system under the physiological conditions in the presence of biosynthetic factors was constructed. For the large subunit, we succeeded in reconstituting subunits with protein synthesis activity from individually purified ribosomal proteins and rRNA. We also developed a PURE system based on transcribed tRNA molecules.
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