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Fabrication of three dimensional tissue structures by micromanipulation of magnetic fibers

Research Project

Project/Area Number 16H02322
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Intelligent mechanics/Mechanical systems
Research InstitutionMeijo University

Principal Investigator

Fukuda Toshio  名城大学, 理工学部, 教授 (70156785)

Co-Investigator(Kenkyū-buntansha) 市川 明彦  名城大学, 理工学部, 准教授 (20377823)
竹内 大  名古屋大学, 工学研究科, 特任助教 (20713374)
中島 正博  名古屋大学, 工学研究科, 助教 (80377837)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥45,110,000 (Direct Cost: ¥34,700,000、Indirect Cost: ¥10,410,000)
Fiscal Year 2018: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2017: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Keywords3次元細胞組織 / 自己組織的組み立て / マイクロマニピュレーション / 磁場操作 / 局所磁場操作 / 磁力操作 / 3次元細胞構築 / マグネット / 3次元細胞構築 / マグネットファイバー
Outline of Final Research Achievements

Without vascular networks, three dimensional tissues populated with cells cannot maintain a living condition. To construct 3D tissues, a well-organized vascular network is required. In this research, a new method for constructing a hepatic lobule-like vascular network in 3D cellular structure by using magnetic fields is proposed. To realize channel networks that mimic hepatic lobule, steel rods and magnetic fibers were utilized as a sacrificial mold in fibrin gel. The steel rods and fibers were connected by magnetic fields generated from magnetic tweezers.
A tissue made of fibrin gel and rat liver cells or spheroids with 3D channel network was cultured for a week. The viability of cells in the tissue was checked and higher viability was achieved when the cells were placed near the channel structure. The hepatic function was not decreased even if ferrite particles were mixed inside the fabricated tissue for magnetic manipulation.

Academic Significance and Societal Importance of the Research Achievements

現在、臓器移植希望登録者に対する移植件数は僅か数%と移植件数が圧倒的に少なく、この問題を解決する方法として再生医療は大きな注目を集めている。しかしながら、血管を含む3次元の組織を生体外で構築するためには、まだ課題も多い。また、3次元の細胞組織を生体外にて作製することで、薬剤の効能試験など臓器モデルとして使用することが可能であり、実験動物を使った薬剤テストを削減することにもつながる。このように、本研究で目的としている生体外3次元細胞組織は、実現すれば社会的なインパクトも大きく、臓器機能の発現原理の解明など学術的な意義も併せて大きなものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (38 results)

All 2018 2017 2016

All Journal Article (6 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 6 results) Presentation (31 results) (of which Int'l Joint Research: 17 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] In Vitro Mimicking the Morphology of Hepatic Lobule Tissue Based on Ca-Alginate Cell Sheets2018

    • Author(s)
      Zeyang Liu, Minmin Lu, Masaru Takeuchi, Yue Tao, Yasuhisa Hasegawa, Qiang Huang, Toshio Fukuda
    • Journal Title

      Biomedical Materials

      Volume: 13 Issue: 3 Pages: 035004-035004

    • DOI

      10.1088/1748-605x/aaa4c4

    • NAID

      120006482564

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Microrobotic Assembly of Shape-customized Three-Dimensional Microtissues based on Surface Tension Driven Self-alignment2018

    • Author(s)
      Huaping Wang, Jianing Li, Juan Cui, Zhiqiang Zheng, Qing Shi, Tao Sun, Qiang Huang, Toshio Fukuda
    • Journal Title

      IEEE Transactions on Nanotechnology

      Volume: 17 Issue: 4 Pages: 684-687

    • DOI

      10.1109/tnano.2018.2806385

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Automated Fluidic Assembly of Microvessel-Like Structures Using a Multi-Micromanipulator System2018

    • Author(s)
      Xiaoming Liu, Huaping Wang, Tao Sun, Ning Yu, Qiang Huang, Toshio Fukuda
    • Journal Title

      IEEE/ASME Transactions on Mechatronics

      Volume: 23 Issue: 2 Pages: 667-678

    • DOI

      10.1109/tmech.2018.2796182

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Multi-layered Channel Patterning by Local Heating of Hydrogels2017

    • Author(s)
      M. Takeuchi, T. Oya, A. Ichikawa, A. Hasegawa, M. Nakajima, Y. Hasegawa, T. Fukuda
    • Journal Title

      IEEE Robotics and Automation Letters

      Volume: 2 Issue: 2 Pages: 958-963

    • DOI

      10.1109/lra.2017.2655625

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 3D Hepatic Lobule-Like Tissue Constructs by Cell Microcapsule Technology2017

    • Author(s)
      Z. Liu, M. Takeuchi, M. Nakajima, C. Hu, Y. Hasegawa, Q. Huang, T. Fukuda
    • Journal Title

      Acta Biomaterialia

      Volume: 50 Pages: 178-187

    • DOI

      10.1016/j.actbio.2016.12.020

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Shape-controlled high cell-density microcapsules by electrodeposition2016

    • Author(s)
      Z. Liu, M. Takeuchi, M. Nakajima, Y. Hasegawa, Q. Huang, T. Fukuda
    • Journal Title

      Acta Biomaterialia

      Volume: 37 Pages: 93-100

    • DOI

      10.1016/j.actbio.2016.03.045

    • NAID

      120005868249

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Studing Making Micro Structure with Biodegradable Materials2018

    • Author(s)
      Taro Kozuka, Masaru Takeuchi, Akiyuki Hasegawa, Eunhye Kim, Akihiko Ichikawa and Toshio Fukuda
    • Organizer
      29th 2018 International Symposium on Micro-NanoMechatronics and Human Science
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cultured Muscles with Tendon Structures for Modular Bio-Actuators2018

    • Author(s)
      Takuto Nomura, Masaru Takeuchi, Akihiko Ichikawa, Eunhye Kim, Akiyuki Hasegawa and Toshio Fukuda
    • Organizer
      29th 2018 International Symposium on Micro-NanoMechatronics and Human Science
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Magnetic Assembly of Hydrogel Fiber for Constructing Three-dimensional Hepatic Lobule-like Channel Network2018

    • Author(s)
      Eunhye Kim, Masaru Takeuchi, Taro Kozuka, Takuto Nomura, Ryohei Sakurai, Akiyuki Hasegawa, Akihiko Ichikawa and Toshio Fukuda
    • Organizer
      29th 2018 International Symposium on Micro-NanoMechatronics and Human Science
    • Related Report
      2018 Annual Research Report
  • [Presentation] Cell Culture on Biodegradable Microstructures using On-chio Fabrication of Photocrosslinkable Hydrogel2018

    • Author(s)
      Masaru Takeuchi, Taro Kozuka, Eunhye Kim, Akihiko Ichikawa, Toshio Fukuda and Yasuhisa Hasegawa
    • Organizer
      International Conference on Manipulation, Automation and Robotics at Small Scales
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Construction of Hepatic Lobule-like Vascular Network by using Magnetic fields2018

    • Author(s)
      Eunhye Kim, Masaru Takeuchi, Wataru Atou, Yuta Iwamoto, Takuto Nomura, Taro Kozuka, Akiyuki Hasegawa, Akihiko Ichikawa, and Toshio Fukuda
    • Organizer
      2018 IEEE International Conference on Robotics and Automation
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生分解性材料を用いたマイクロ構造体作製の研究2018

    • Author(s)
      小塚太郎、竹内大、長谷川明之、金恩恵、市川明彦、福田敏男
    • Organizer
      第36回日本ロボット学会学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 7本のマグネットツイザーを用いた血管構造体作製2018

    • Author(s)
      金恩恵、竹内大、櫻井陵平、小塚太郎、野村匠永、長谷川明之、市川明彦、 福田敏男
    • Organizer
      第36回日本ロボット学会学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] モジュラーバイオアクチュエータのための腱構造付き培養筋の作製2018

    • Author(s)
      野村匠永、市川明彦、竹内大、金恩恵、長谷川明之、福田敏男
    • Organizer
      第36回日本ロボット学会学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 生分解性材料を用いたマイクロ構造体作製の研究2018

    • Author(s)
      小塚太郎、竹内大、長谷川明之、金恩恵、市川明彦、福田敏男
    • Organizer
      ロボティクス・メカトロニクス講演会 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] トロイダル構造体を用いた多自由度骨格筋アクチュエータの構築2018

    • Author(s)
      野村匠永、市川明彦、竹内大、金恩恵、長谷川明之、福田敏男
    • Organizer
      ロボティクス・メカトロニクス講演会 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] マグネットファイバーによる肝小葉を模した血管構造の製作2018

    • Author(s)
      金恩恵、竹内大、阿藤航、小塚太郎、野村匠永、岩本佑太、長谷川明之、市川明彦、福田敏男
    • Organizer
      ロボティクス・メカトロニクス講演会 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] 筋細胞内包ゲルファイバーの集積による筋線維束形成の試み2018

    • Author(s)
      長谷川明之、岩本佑太、竹内大、金恩恵、市川明彦、福田敏男
    • Organizer
      ロボティクス・メカトロニクス講演会 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] Magnetic self-assembly of Toroidal Microstructures by Shaking2017

    • Author(s)
      Masaru Takeuchi, Mamoru Hattori, Akihiko Ichikawa, Toshio Fukuda, Yasuhisa Hasegawa
    • Organizer
      28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Construction of Vascular Network in 3D Cellular Structure by using Magnetic Field2017

    • Author(s)
      Eunhye Kim, Masaru Takeuchi, Akihiko Ichikawa, Toshio Fukuda
    • Organizer
      28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In Vitro Mimic of Hepatic Lobule Tissue using Ca-alginate Cell-containing Hydrogel Modules2017

    • Author(s)
      Zeyang Liu, Minmin Lu, Masaru Takeuchi, Yue Tao, Yasuhisa Hasegawa, Qiang Huang, Toshio Fukuda
    • Organizer
      28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication Consideration of Three-dimensional Skeletal Muscle Fiber using Biomaterial2017

    • Author(s)
      Yuta Iwamoto, Kenichi Ohara, Masaru Takeuchi, Akihiko Ichikawa, Toshio Fukuda
    • Organizer
      28th 2017 International Symposium on Micro-NanoMechatronics and Human Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bio-fabrication of Ca-alginate cell sheets with the electrodeposition method for hepatic lobule tissue reconstructs2017

    • Author(s)
      Zeyang Liu, Masaru Takeuchi, Yasuhisa Hasegawa, Toshio Fukuda, Qiang Huang
    • Organizer
      21th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] On-Chip Fabrication of Movable Toroidal Cell Structures Using Photo-Crosslinkable Biodegradable Hydrogel2017

    • Author(s)
      Masaru Takeuchi, Yuki Nakamura, Akihiko Ichikawa, Akiyuki Hasegawa, Yasuhisa Hasegawa, Toshio Fukuda
    • Organizer
      2017 IEEE/RSJ International Conference on Intelligent Robots and Systems
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication of Multilayered Hepatic Lobule Tissues Using Ca-Alginate Hydrogel Platforms2017

    • Author(s)
      Zeyang Liu, Minmin Lu, Masahiro Nakajima, Masaru Takeuchi, Yasuhisa Hasegawa, Toshio Fukuda, Qiang Huang
    • Organizer
      17th IEEE International Conference on Nanotechnology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ハイドロゲルの局所加熱を用いた多層細胞構造体の作製2017

    • Author(s)
      大矢智之,竹内大,長谷川明之,市川明彦,中島正博,長谷川泰久,福田敏男
    • Organizer
      ロボティクス・メカトロニクス講演会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 筋細胞内包ゲルファイバーの集積化による骨格筋組織の模倣2017

    • Author(s)
      岩本佑太,大原賢一,竹内大,市川明彦,福田敏男
    • Organizer
      ロボティクス・メカトロニクス講演会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] マグネットツイザーを用いた3次元細胞構造体構築2017

    • Author(s)
      Kim Eunhye,阿藤 航,小塚 太郎,野村 匠永,岩本 佑太,市川 明彦,竹内 大,福田 敏男
    • Organizer
      第18回 計測自動制御学会システムインテグレーション部門講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] トロイダルハイドロゲルを用いた多自由度骨格筋アクチュエータの構築2017

    • Author(s)
      野村 匠永,福田 敏男,市川 明彦,竹内 大,Kim Eunhye,岩本 佑太,小塚 太郎,阿藤 航
    • Organizer
      第18回 計測自動制御学会システムインテグレーション部門講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 折り目を有した細胞足場体への磁性マイクロ構造体の磁場操作アセンブリ2016

    • Author(s)
      中島 正博,服部 守,長谷川 明之,竹内 大,福田 敏男
    • Organizer
      第17回計測自動制御学会システムインテグレーション部門
    • Place of Presentation
      札幌
    • Year and Date
      2016-12-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Selective Channel Fabrication by Local Heating for Cell Patterning2016

    • Author(s)
      T. Oya, M. Takeuchi, K. Ohara, A. Ichikawa, M. Nakajima, T. Fukuda
    • Organizer
      2016 International Symposium on Micro-NanoMechatronics and Human Science
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2016-11-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Assembly of Hepatic Lobule-like Microtissue with Repetitive Single-Step Contact Manipulation2016

    • Author(s)
      Z. Liu, M. Takeuchi, M. Nakajima, T. Fukuda, Y. Hasegawa, Q. Huan
    • Organizer
      2016 International Symposium on Micro-NanoMechatronics and Human Science
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2016-11-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication Method of Artificial Integrated Muscle Fiber toward Bio-actuator2016

    • Author(s)
      Y. Iwamoto, K. Ohara, M. Takeuchi, A. Ichikawa, T. Fukuda
    • Organizer
      2016 International Symposium on Micro-NanoMechatronics and Human Science
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2016-11-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cell Sheets Fabrication with Mimicking Morphology of Liver Lobule Tissue by Electrodeposition2016

    • Author(s)
      M. Lu, Z. Liu, M. Nakajima, M. Takeuchi, Y. Hasegawa, T. Fukuda, Q. Huang
    • Organizer
      2016 International Symposium on Micro-NanoMechatronics and Human Science
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2016-11-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Self-Assembly of Toroidal Magnetic Microstructures towards in Vitro Cell Structures2016

    • Author(s)
      M. Takeuchi, M. Hattori, A. Ichikawa, K. Ohara, M. Nakajima, T. Fukuda, Y. Hasegawa Q. Huang
    • Organizer
      2016 IEEE/RSJ International Conference on Intelligent Robots and Systems
    • Place of Presentation
      Deajeon, Korea
    • Year and Date
      2016-10-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Self-assembly of magnetized microstructures for in vitro cell systems2016

    • Author(s)
      M. Hattori, A. Ichikawa, K. Ohara, T. Fukuda, M. Takeuchi, M. Nakajima, Y. Hasegawa Q. Huang
    • Organizer
      2016 IEEE International Conference on Mechatronics and Automation
    • Place of Presentation
      Harbin, China
    • Year and Date
      2016-08-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 局所的熱融解による生体親和性ゲルの選択的チャネルパターン2016

    • Author(s)
      大矢 智之,竹内 大,大原 賢一,市川 明彦,中島 正博,長谷川 泰久,福田 敏男
    • Organizer
      ロボティクス・メカトロニクス講演会2016
    • Place of Presentation
      横浜
    • Year and Date
      2016-06-08
    • Related Report
      2016 Annual Research Report
  • [Book] 1.Microfluidics for Mass Measurement of Miniature Object Like Single Cell and Single MicroParticle2017

    • Author(s)
      M. H. Rahman, M. R. Ahmad, M. Takeuchi, M. Nakajima, Y. Hasegawa, T. Fukuda
    • Total Pages
      23
    • Publisher
      Springer
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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