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Biofabrication of functional 3D tissues

Research Project

Project/Area Number 16H02423
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionOsaka University

Principal Investigator

TAYA MASAHITO  大阪大学, 基礎工学研究科, 教授 (60144127)

Co-Investigator(Kenkyū-buntansha) 小嶋 勝  大阪大学, 基礎工学研究科, 助教 (00533647)
境 慎司  大阪大学, 基礎工学研究科, 教授 (20359938)
松永 民秀  名古屋市立大学, 大学院薬学研究科, 教授 (40209581)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000)
Fiscal Year 2018: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2017: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2016: ¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
Keywordsバイオプリンティング / 組織工学 / 再生医療 / 医用化学工学 / ヒドロゲル / 3Dプリンター / 3次元組織 / 生体材料 / バイオプリンター
Outline of Final Research Achievements

In this research, we aimed to establish 3D bioprinting technology based on visible light curing inks and bioprinters for them for fabricating functional 3D tissue construction. As the visible light curing ink, we used polymers possessing phenolic hydroxyl moieties. Visible light responsive photoredox catalyst was used for inducing hydrogelation. We succeeded in fabrication of 3D hydrogel constructs including cells and a blood vessel-like perfusable structure without affecting the survival of cells. Furthermore, it was revealed that it is possible to achieve proliferation of cells in the hydrogel constructs as well as differentiation induction from human adipose-derived stem cells. As such, we were able to succeed in developing a new 3D bioprinting technology.

Academic Significance and Societal Importance of the Research Achievements

3Dバイオプリンティングは、生体外にて人工的に機能的な組織を構築するためのキーテクノロジーとして期待されている。本研究では、それに寄与する検討に取り組んだ。そして、新たに光硬化性インクを用いた細胞含有三次元構造体の構築法の開発に成功するとともに、細胞源として期待される脂肪由来幹細胞を使って、その有用性を実証した。これらは、簡単な装置や単純な手法で達成されたことから、今後さまざまな研究者に利用され、再生医療をターゲットとする組織工学だけでなく、細胞の機能などを調べる用途などにも利用されると期待される。さらに、再生医療の進展に寄与する技術の開発に成功したことから、その成功は社会的にも意義が高い。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (47 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (37 results) (of which Int'l Joint Research: 12 results,  Invited: 9 results)

  • [Int'l Joint Research] Clermont Auvergne University(フランス)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Characterization of encapsulated cells within hyaluronic acid and alginate microcapsules produced via horseradish peroxidase-catalyzed crosslinking2019

    • Author(s)
      Khanmohammadi Mehdi、Sakai Shinji、Taya Masahito
    • Journal Title

      Journal of Biomaterials Science, Polymer Edition

      Volume: 30 Issue: 4 Pages: 295-307

    • DOI

      10.1080/09205063.2018.1562637

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Horseradish peroxidase-catalyzed hydrogelation consuming enzyme-produced hydrogen peroxide in the presence of reducing sugars2019

    • Author(s)
      Gantumur Enkhtuul、Sakai Shinji、Nakahata Masaki、Taya Masahito
    • Journal Title

      Soft Matter

      Volume: 15 Issue: 10 Pages: 2163-2169

    • DOI

      10.1039/c8sm01839a

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Peroxidase-catalyzed microextrusion bioprinting of cell-laden hydrogel constructs in vaporized ppm-level hydrogen peroxide2018

    • Author(s)
      Sakai Shinji、Mochizuki Kei、Qu Yanfei、Mail Matthew、Nakahata Masaki、Taya Masahito
    • Journal Title

      Biofabrication

      Volume: 10 Issue: 4 Pages: 045007-045007

    • DOI

      10.1088/1758-5090/aadc9e

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cross-Linking Building Blocks Using a “Boronate Bridge” to Build Functional Hybrid Materials2018

    • Author(s)
      Nakahata Masaki、Sakai Shinji
    • Journal Title

      ChemNanoMat

      Volume: 5 Issue: 2 Pages: 141-151

    • DOI

      10.1002/cnma.201800435

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biofabrication offers future hope for tackling various obstacles and challenges in tissue engineering and regenerative medicine: A Prospective2018

    • Author(s)
      Mir Tanveer Ahmad、Iwanaga Shintaroh、Kurooka Taketoshi、Toda Hideki、Sakai Shinji、Nakamura Makato
    • Journal Title

      International Journal of Bioprinting

      Volume: 5 Issue: 1 Pages: 1-7

    • DOI

      10.18063/ijb.v5i1.153

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visible Light-Induced Hydrogelation of an Alginate Derivative and Application to Stereolithographic Bioprinting Using a Visible Light Projector and Acid Red2018

    • Author(s)
      Sakai Shinji、Kamei Hidenori、Mori Toko、Hotta Tomoki、Ohi Hiromi、Nakahata Masaki、Taya Masahito
    • Journal Title

      Biomacromolecules

      Volume: 19 Issue: 2 Pages: 672-679

    • DOI

      10.1021/acs.biomac.7b01827

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differentiation potential of human adipose stem cells bioprinted with hyaluronic acid/gelatin-based bioink through microextrusion and visible light-initiated crosslinking2017

    • Author(s)
      Sakai Shinji、Ohi Hiromi、Hotta Tomoki、Kamei Hidenori、Taya Masahito
    • Journal Title

      Biopolymers

      Volume: 109 Issue: 2

    • DOI

      10.1002/bip.23080

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Drop-On-Drop Multimaterial 3D Bioprinting Realized by Peroxidase-Mediated Cross-Linking2017

    • Author(s)
      Sakai Shinji、Ueda Kohei、Gantumur Enkhtuul、Taya Masahito、Nakamura Makoto
    • Journal Title

      Macromolecular Rapid Communications

      Volume: 39 Issue: 3 Pages: 1700534-1700534

    • DOI

      10.1002/marc.201700534

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impact of immobilizing of low molecular weight hyaluronic acid within gelatin-based hydrogel through enzymatic reaction on behavior of enclosed endothelial cells2017

    • Author(s)
      Khanmohammadi Mehdi、Sakai Shinji、Taya Masahito
    • Journal Title

      International Journal of Biological Macromolecules

      Volume: 97 Pages: 308-316

    • DOI

      10.1016/j.ijbiomac.2016.12.088

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] The influence of STAT5 activation by celecoxib on differentiation of human induced pluripotent stem cells into hepatocytes2018

    • Author(s)
      Hiroki Okumura, Anna Nakanishi, Tadahiro Hashita, Takahiro Iwao, Tamihide Matsunaga
    • Organizer
      ISSCR Annual Meeting 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In Vitro Cholestatic Drug-Induced Liver Injury Evaluation System Using Human iPS Cell-Derived Hepatocytes2018

    • Author(s)
      Yoko Sakai, Takahiro Iwao, Takeshi Susukida, Akinori Takemura, Takumi Nukaga, Shuichi Sekine, Kousei Ito, Tamihide Matsunaga
    • Organizer
      2018 International Meeting on 22nd MDO and 33rd JSSX
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Micro-extrusion bioprinting mediated by horseradish peroxidase2018

    • Author(s)
      S Sakai, K Mochizuki, M Taya
    • Organizer
      23rd International Congress of Chemical and Process Engineering: CHISA 2018 Prague
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biofabrication of 3D cardiac tissues with bio-printing and bio-assembly technologies2018

    • Author(s)
      Y Hamada, S Iwanaga, Y Tsukamoto, K Sugimoto, S Sakai, M Nakamura
    • Organizer
      2018 TERMIS World Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Horseradish peroxidase-mediated micro-extrusion bioprinting in air containing vaporized hydrogen peroxide2018

    • Author(s)
      S Sakai, K Mochizuki, M Taya
    • Organizer
      International Conference on Biofabricaton 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multi-ink inkjet bioprinting for cell-patterning through enzymatic cross-linking2018

    • Author(s)
      E Gantumur, M Kimura, S Sakai, M Nakahata, M Taya
    • Organizer
      International Conference on Biofabricaton 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fabrication of cell fibers with hollow alginate hydrogel molds for bioassembly of large-sized tissues and organs2018

    • Author(s)
      T Muroe, S Iwanaga, Y Aso, S Sakai, T Kurooka, M Nakamura
    • Organizer
      International Conference on Biofabricaton 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 酵素架橋とインクジェットの融合による3Dバイオプリンティング技術の開発2018

    • Author(s)
      境 慎司, 中村 真人
    • Organizer
      第121回 日本画像学会年次大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] インクジェット(5-2) 応用/3Dプリンティング/バイオプリンティング2018

    • Author(s)
      中村 真人, 岩永 進太郎, 浜田 裕太, 黒岡 武俊, 境 慎司
    • Organizer
      第121回 日本画像学会年次大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 組織構築に向けた中空アルギン酸ゲルモールドを用いた細胞ファイバーの作製2018

    • Author(s)
      室江 拓哉, 岩永 進太郎, 阿曽 祐也, 境 慎司, 黒岡 武俊, 中村 真人
    • Organizer
      第5回富山・バーゼル医薬品研究開発シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 3Dバイオプリンティング2018

    • Author(s)
      境 慎司
    • Organizer
      第70回 日本生物工学会大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 複数素材が適用可能なインクジェット式バイオプリンターを利用した細胞パターニングの構築2018

    • Author(s)
      Gantumur Enkhtuul, 木村 美夢, 境 慎司, 中畑 雅樹, 田谷 正仁
    • Organizer
      第50回 化学工学会秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] キトサンナノファイバーを用いる3Dバイオプリンティング2018

    • Author(s)
      服部 晃治, 境 慎司, 中畑 雅樹, 田谷 正仁
    • Organizer
      第50回 化学工学会秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 光回復酵素と可視光を用いてパターニング可能な培養基材の開発2018

    • Author(s)
      小川 健斗, 境 慎司, 中畑 雅樹, 山元 淳平, 岩井 成憲, 田谷 正仁
    • Organizer
      第50回 化学工学会秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 西洋ワサビペルオキシダーゼを使った細胞培養用エレクトロスピニング不織布の作製2018

    • Author(s)
      古野 琴子, 境 慎司, 鈴木 啓一郎, 田谷 正仁
    • Organizer
      第50回 化学工学会秋季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 西洋わさび由来ペルオキシダーゼ代替触媒としてのヘミンの細胞毒性低減とその応用2018

    • Author(s)
      古川 翔太, 境 慎司, 田谷 正仁
    • Organizer
      第4回 日本生物工学会西日本支部 講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] キトサンを用いた三次元多孔質構造の3Dプリンティングとその応用2018

    • Author(s)
      本田 尚之, 境 慎司, 田谷 正仁
    • Organizer
      第4回 日本生物工学会西日本支部 講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] キトサンを含むバイオインクを用いた3Dプリンティング2018

    • Author(s)
      山本 翔太, 境 慎司, 田谷 正仁
    • Organizer
      第4回 日本生物工学会西日本支部 講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 金属-配位子相互作用により架橋されるゼラチンの合成とインクジェットプリンティングへの応用2018

    • Author(s)
      平岡功匡, 中畑 雅樹, 境 慎司, 田谷 正仁
    • Organizer
      化学工学会 中国四国支部・関西支部合同徳島大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Development of Bioinks Containing Nanofibrillated Chitosan for Extrusion 3D Bioprinting2018

    • Author(s)
      S Sakai, K Hattori, M Taya
    • Organizer
      The 14th Asian Congress on Biotechnology
    • Related Report
      2018 Annual Research Report
  • [Presentation] Horseradish Peroxidase-Catalyzed Hydrogelation Consuming Enzyme-Produced Hydrogen Peroxide in the Presence of Reducing Sugars2018

    • Author(s)
      E Gantumur, S Sakai, M Taya
    • Organizer
      The 14th Asian Congress on Biotechnology
    • Related Report
      2018 Annual Research Report
  • [Presentation] Inkjet plus enzyme-mediated bioprinting for controlled cell immobilization on substrates2018

    • Author(s)
      M Kimura, E Gantumur, S Sakai, M Taya
    • Organizer
      The 14th Asian Congress on Biotechnology
    • Related Report
      2018 Annual Research Report
  • [Presentation] Development of nanofiber nonwoven fabric applicable for genome editing in vivo2018

    • Author(s)
      K Furuno, S Sakai, K Suzuki, M Taya
    • Organizer
      The 14th Asian Congress on Biotechnology
    • Related Report
      2018 Annual Research Report
  • [Presentation] 過酸化水素蒸気中での酵素による架橋形成反応にもとづく3Dバイオプリンティング技術の開発2018

    • Author(s)
      境 慎司,望月佳,田谷正仁
    • Organizer
      化学工学会 第83年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 微量の過酸化水素を含む空気とHRPを用いた微量押し出し式3D-Bioprinitng技術の開発2017

    • Author(s)
      望月佳,境慎司,中畑雅樹,田谷正仁
    • Organizer
      第39回日本バイオマテリアル学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Peroxidase-catalyzed microextrusion bioprinting in vaporized ppm-level hydrogen peroxide2017

    • Author(s)
      Kei Mochizuki, Shinji Sakai, Nakahata Masaki, Masahito Taya
    • Organizer
      2017 International Symposium of Materials on Regenerative Medicine (ISOMRM)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Visible light induced alginate-based hydrogel formation catalyzed by ruthenium complex and application to 3D printing2017

    • Author(s)
      Hidenori Kamei, Touko Mori, Shinji Sakai, Masahito Taya
    • Organizer
      2017 International Symposium of Materials on Regenerative Medicine (ISOMRM)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Bioprinting of human adipose stem cell-laden hyaluronic acid/gelatin-based hydrogel constructs through micro-extrusion and visible light-irradiation2017

    • Author(s)
      Hiromi Ohi、Shinji Sakai、Masahito Taya
    • Organizer
      2017 International Symposium of Materials on Regenerative Medicine (ISOMRM)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Drop-on-drop 3D printing through horseradish peroxidase-catalyzed hydrogelation2017

    • Author(s)
      Shinji Sakai, Kohei Ueda, Masahito Taya, Makoto Nakamura
    • Organizer
      28th Annual Conference of the European Society for Biomaterials
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Cell-laden hydrogel bioprinting using enzymatic hydrogelation and inkjetting2017

    • Author(s)
      Shinji Sakai, Gantumur Enkhtuul, Kohei Ueda, Masahito Taya
    • Organizer
      3rd Latin-America Symposium on Microencapsulation
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] フェノール性水酸基導入高分子を使ったバイオプリンティング2016

    • Author(s)
      境慎司、田谷正仁、中村真人
    • Organizer
      2016年 化学工学会秋季大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 西洋わさび由来ペルオキシダーゼによる架橋反応を利用した3Dバイオプリンティング2016

    • Author(s)
      境慎司、田谷正仁、中村真人
    • Organizer
      第68回日本生物工学会大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Effect of encapsulation in hydrogel sheath through cell cycle progression2016

    • Author(s)
      Tomoki Hotta, Shinji Sakai and Masahito Taya
    • Organizer
      24th International Conference on Bioencapsulation
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cell-selective encapsulation in hydrogel sheaths using antibody conjugated HRP2016

    • Author(s)
      Mikako Sengoku, Shinji Sakai and Masahito Taya
    • Organizer
      24th International Conference on Bioencapsulation
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Application of enzymatic reactions for hydrogel-based biomedical engineering2016

    • Author(s)
      Shinji Sakai
    • Organizer
      International Conference on Bio-Fabrication and Bio-monitoring 2016 (ICBB 2016)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cell encapsulation in microparticles by inkjetting and enzymatic cross-linking2016

    • Author(s)
      Gantumur Enkhtuul, Shinji Sakai, Masahito Taya
    • Organizer
      The 22nd Symposium of Young Asian Biological Engineers’ Community (YABEC) 2016
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Enzymatic immobilization of low molecular weight hyaluronic acid in gelatin hydrogel: A strategy to promote endothelial cell migration2016

    • Author(s)
      Mehdi Khanmohammadi, Shinji Sakai, Masahito Taya
    • Organizer
      The 22nd Symposium of Young Asian Biological Engineers’ Community (YABEC) 2016
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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