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Time-window of NMDA receptor-dependent LTP during memory formation, consolidation and recall

Research Project

Project/Area Number 16H02455
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurophysiology / General neuroscience
Research InstitutionKyoto University (2017-2018)
Institute of Physical and Chemical Research (2016)

Principal Investigator

Hayashi Yasunori  京都大学, 医学研究科, 教授 (90466037)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥46,020,000 (Direct Cost: ¥35,400,000、Indirect Cost: ¥10,620,000)
Fiscal Year 2018: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2017: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2016: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Keywordsシナプス可塑性 / 記憶・学習 / コフィリン / スパイン / 記憶固定化 / 海馬 / 樹状突起スパイン / 細胞骨格 / 光増感法 / 記憶学習 / 長期記憶 / 記憶痕跡 / 睡眠
Outline of Final Research Achievements

In order to study when and where synaptic plasticity occurs during memory consolidation, we established a system that allows erasure of NMDA-receptor mediated synaptic memory. For this purpose, we established a method to optically inactivate cofilin, a protein that accumulates at the synapse after the induction of synaptic plasticity and stabilizes actin cytoskeleton. By using this method, we can optically erase synaptic potentiation within 30 min after induction. When we tested when synaptic plasticity occurs in an intact animal, we found that it occurs while an animal is at asleep. This indicates that reactivation of hippocampal neurons during sleep induces synaptic plasticity, which is required for memory consolidation.

Academic Significance and Societal Importance of the Research Achievements

本技術を用いれば、記憶固定化の過程に重要なLTPを解除することが可能であり、記憶学習の固定化のメカニズムを解明するための画期的なツールとなると期待される。またそればかりではなく、記憶が異常に亢進している疾患、例えば外傷後ストレス症候群(PTSD)や薬物依存症の治療にも有用ではないかと考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (13 results)

All 2019 2018 2017 Other

All Journal Article (7 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 7 results,  Open Access: 3 results) Presentation (5 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Remarks (1 results)

  • [Journal Article] Autophosphorylation of F-actin binding domain of CaMKIIβ is required for fear learning2019

    • Author(s)
      Kim Karam、Suzuki Akio、Kojima Hiroto、Kawamura Meiko、Miya Ken、Abe Manabu、Yamada Ikuko、Furuse Tamio、Wakana Shigenaru、Sakimura Kenji、Hayashi Yasunori
    • Journal Title

      Neurobiology of Learning and Memory

      Volume: 157 Pages: 86-95

    • DOI

      10.1016/j.nlm.2018.12.003

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Reciprocal activation within a kinase-effector complex underlying persistence of structural LTP.2019

    • Author(s)
      1.Saneyoshi T, Matsuno H, Suzuki A, Murakoshi H, Hedrick NG, Agnello E, O’Connell R, Stratton MM, Yasuda R, Hayashi Y
    • Journal Title

      Neuron

      Volume: in press

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Structure of monomeric full-length ARC sheds light on molecular flexibility, protein interactions, and functional modalities2018

    • Author(s)
      Hallin Erik I.、Eriksen Maria S.、Baryshnikov Sergei、Nikolaienko Oleksii、Gr?dem Sverre、Hosokawa Tomohisa、Hayashi Yasunori、Bramham Clive R.、Kursula Petri
    • Journal Title

      Journal of Neurochemistry

      Volume: 147 Issue: 3 Pages: 323-343

    • DOI

      10.1111/jnc.14556

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] ERK5 Phosphorylates Kv4.2 and Inhibits Inactivation of the A-Type Current in PC12 Cells.2018

    • Author(s)
      Yurina Kashino, Yutaro Obara, Yosuke Okamoto, Takeo Saneyoshi, Yasunori Hayashi, Kuniaki Ishii.
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 19(7) Issue: 7 Pages: 2008-2008

    • DOI

      10.3390/ijms19072008

    • NAID

      120006559401

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hippocampus-Dependent Goal Localization by Head-Fixed Mice in Virtual Reality2017

    • Author(s)
      Sato Masaaki、Kawano Masako、Mizuta Kotaro、Islam Tanvir、Lee Min Goo、Hayashi Yasunori
    • Journal Title

      eneuro

      Volume: 4 Issue: 3 Pages: 1-17

    • DOI

      10.1523/eneuro.0369-16.2017

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Photomarking Relocalization Technique for Correlated Two-Photon and Electron Microcopy Imaging of Single Stimulated Synapses2017

    • Author(s)
      Bosch Miquel、Castro Jorge、Sur Mriganka、Hayashi Yasunori
    • Journal Title

      Methods Mol. Biol.

      Volume: 1538 Pages: 185-214

    • DOI

      10.1007/978-1-4939-6688-2_14

    • ISBN
      9781493966868, 9781493966882
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Interplay of enzymatic and structural functions of CaMKII in long‐term potentiation2017

    • Author(s)
      Karam Kim, Takeo Saneyoshi, Tomohisa Hosokawa, Kenichi Okamoto, Yasunori Hayashi
    • Journal Title

      Journal of neurochemistry

      Volume: 6 Issue: 6 Pages: 959-959

    • DOI

      10.1111/jnc.13672

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Why is CaMKII so abundant at synapse?2019

    • Author(s)
      Hayashi Y.
    • Organizer
      OIST Membran Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Reciprocal activation within a kinase-effector complex underlying LTP2019

    • Author(s)
      Hayashi Y.
    • Organizer
      Niigata University Brain Research Institute Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Reciprocal activation within a kinase-effector complex underlying LTP2018

    • Author(s)
      Hayashi Y.
    • Organizer
      Asia-Pacific Society for Neurochemistry
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Reciprocal activation within a kinase-effector complex underlying LTP2018

    • Author(s)
      Hayashi Y.
    • Organizer
      ax Planck Institute for Biochemistry
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] A positive feedback mechanism supporting persistent signaling during hippocampal LTP2018

    • Author(s)
      Hayashi Y.
    • Organizer
      Hong Kong University of Science and Technology
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Remarks]

    • URL

      http://glutamate.med.kyoto-u.ac.jp

    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2024-12-25  

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