Risk estimation of pancreatic adenocarcinomas based on epigenome-wide association study
Project/Area Number |
16H02472
|
Research Category |
Grant-in-Aid for Scientific Research (A)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Tumor diagnostics
|
Research Institution | Keio University |
Principal Investigator |
Kanai Yae 慶應義塾大学, 医学部(信濃町), 教授 (00260315)
|
Co-Investigator(Kenkyū-buntansha) |
山地 太樹 国立研究開発法人国立がん研究センター, 社会と健康研究センター, 室長 (10466203)
新井 恵吏 慶應義塾大学, 医学部(信濃町), 講師 (40446547)
伊藤 秀美 愛知県がんセンター(研究所), がん情報・対策研究分野, 分野長 (90393123)
|
Research Collaborator |
YOSHIDA Teruhiko
GOTOH Masahiro
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2018: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2017: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2016: ¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
|
Keywords | エピゲノム / エピゲノムワイド関連解析 (EWAS) / 膵がん / DNAメチル化 / エピゲノムワイド関連解析 / リスク診断 |
Outline of Final Research Achievements |
The aim of this study was to establish the criteria for risk estimation of pancreatic cancer using blood samples based the research strategy of epigenome-wide association study. Using genome-wide DNA methylation analysis, 46,924 CpG sites showing significant differences of DNA methylation levels between blood samples of individuals who will suffer pancreatic cancer later and age and sex-matched control blood samples and simultaneously showing such differences of DNA methylation levels between pancreatic cancer tissue and normal pancreatic tissue samples were identified. Many of genes showing such DNA methylation alterations in both blood and tissue samples participated in stemness and/or cell proliferation and were potential tumor-related genes. These data indicated that cancer-prone DNA methylation profile can be detected even in blood samples and revealed the feasibility of pancreatic cancer risk estimation using blood samples during health checkup.
|
Academic Significance and Societal Importance of the Research Achievements |
本研究で同定したリスク診断マーカーCpG部位の、DNAメチル化率の診断閾値を組み合わせることによって、健診等の血液検査によって将来の膵がんの発生リスクを予測するための診断基準を確立できる見込みである。確立した膵がん発生リスク診断基準について、適切に知財を確保する。さらに、研究代表者等が企業と共同研究開発し既に特許の成立している、健診機関にも普及が容易で血液検査に適した、高速液体クロマトグラフィーを基盤とするDNAメチル化診断システムに適合させ、キット化する。これにより、予防先制医療が当然のこととなる近い将来に、発がんリスク診断と介入予防の社会実装を図れると期待される。
|
Report
(4 results)
Research Products
(41 results)
-
[Journal Article] Genome-wide DNA methylation profile of early-onset endometrial cancer: Its correlation with genetic aberrations and comparison with late-onset endometrial cancer.2019
Author(s)
Makabe T, Arai E, Hirano T, Ito N, Fukamachi Y, Takahashi Y, Hirasawa A, Yamagami W, Susumu N, Aoki D, Kanai Y.
-
Journal Title
Carcinogenesis
Volume: 印刷中
Issue: 5
Pages: 1-13
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] The Japanese Society of Pathology Guidelines on the handling of pathological tissue samples for genomic research: Standard operating procedures based on empirical analyses.2018
Author(s)
Kanai Y, Nishihara H, Miyagi Y, Tsuruyama T, Taguchi K, Katoh H, Takeuchi T, Gotoh M, Kuramoto J, Arai E, Ojima H, Shibuya A, Yoshida T, Akahane T, Kasajima R, Morita KI, Inazawa J, Sasaki T, Fukayama M, Oda Y.
-
Journal Title
Pathology International
Volume: 68
Issue: 2
Pages: 63-90
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Epigenome mapping of human normal purified hepatocytes: personal epigenome variation and genome-epigenome correlation.2018
Author(s)
Arai E, Miura F, Totoki Y, Yamashita S, Tian Y, Gotoh M, Ojima H, Nakagawa H, Takahashi Y, Nakamura H, Hama N, Kato M, Kimura H, Suzuki Y, Ito T, Shibata T, Kanai Y.
-
Journal Title
Epigenomics
Volume: 10
Issue: 7
Pages: 955-979
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
[Journal Article] Epigenome mapping of human normal purified hepatocytes: Personal epigenome variation and genome-epigenome correlation.2018
Author(s)
Arai E, Miura F, Totoki Y, Yamashita S, Tian Y, Gotoh M, Ojima H, Nakagawa H, Takahashi Y, Nakamura H, Hama N, Kato M, Kimura H, Suzuki Y, Ito T, Shibata T, Kanai Y.
-
Journal Title
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Genes involved in development and differentiation are commonly methylated in cancers derived from multiple organs: A single-institutional methylome analysis using 1007 tissue specimens.2017
Author(s)
Ohara K, Arai E*, Takahashi Y, Ito N, Shibuya A, Tsuta K, Kushima R, Tsuda H, Ojima H, Fujimoto H, Watanabe S, Katai H, Kinoshita T, Shibata T, Kohno T, Kanai Y.
-
Journal Title
Carcinogenesis
Volume: 38
Pages: 241-251
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] Genome-wide DNA methylation analysis during non-alcoholic steatohepatitis-related multistage hepatocarcinogenesis: comparison with hepatitis virus-related carcinogenesis.2017
Author(s)
Kuramoto J, Arai E, Tian Y, Funahashi N, Hiramoto M, Nammo T, Nozaki Y, Takahashi Y, Ito N, Shibuya A, Ojima H, Sukeda A, Seki Y, Kasama K, Yasuda K, Kanai Y.
-
Journal Title
Carcinogenesis.
Volume: 38
Issue: 3
Pages: 261-270
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
[Presentation] Genome-wide DNA methylation analysis during non-alcoholic steatohepatitis-related hepatocarcinogenesis2019
Author(s)
Ying Tian, Eri Arai, Junko Kuramoto, Satomi Makiuchi, Noboru Tsuda, Hidenori Ojima, Yukihiro Fukamachi, Yoriko Takahashi, Nobuyoshi Hiraoka, Teruhiko Yoshida, Yae Kanai
Organizer
11th American Association for Cancer Research-Japanese Cancer Association Joint Conference
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Genome-wide DNA methylation analysis during nonalcoholic steatohepatitis-related multistage hepatocarcinogenesis: Comparison with hepatitis virus-related carcinogenesis2018
Author(s)
Junko Kuramoto, Eri Arai, Ying Tian, Nobuaki Funahashi, Masaki Hiramoto, Takao Nammo, Yuichi Nozaki, Yoriko Takahashi, Nanako Ito, Ayako Shibuya, Hidenori Ojima, Aoi Sukeda, Yosuke Seki, Kazunori Kasama, Kazuki Yasuda, Yae Kanai
Organizer
American Association for Cancer Research Annual Meeting 2018
Related Report
Int'l Joint Research
-
[Presentation] enome-wide DNA methylation analysis during nonalcoholic steatohepatitis-related multistage hepatocarcinogenesis: Comparison with hepatitis virus-related carcinogenesis,2018
Author(s)
Kuramoto J, Arai E, Tian Y, Funahashi N, Hiramoto M, Nammo T, Nozaki Y, Takahashi Y, Ito N, Shibuya A, Ojima H, Sukeda A, Seki Y, Kasama K, Yasuda K, Kanai Y.
Organizer
The 109th Annual Meeting of the American Association for Cancer Research
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
[Presentation] 肝発がんのエピゲノム機構2016
Author(s)
金井弥栄
Organizer
第57回日本臨床細胞学会総会
Place of Presentation
パシフィコ横浜 (神奈川県横浜市)
Year and Date
2016-05-29
Related Report
Invited
-
-
-
-
-
-
-
-
-